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Information on EC 1.4.3.2 - L-amino-acid oxidase and Organism(s) Bungarus multicinctus and UniProt Accession A8QL51

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EC Tree
     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase
IUBMB Comments
A flavoprotein (FAD).
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This record set is specific for:
Bungarus multicinctus
UNIPROT: A8QL51
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Word Map
The taxonomic range for the selected organisms is: Bungarus multicinctus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
laao, il4i1, l-amino-acid oxidase, l-aao, escapin, head kidney and gill, dolabellanin, l-phenylalanine oxidase, akbu-laao, m-lao, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aromatic L-amino acid oxidase
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-
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L-amino acid oxidase
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-
-
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L-amino acid:O2 oxidoreductase
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L-aminooxidase
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-
-
-
LAAO
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-
-
-
LAO
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-
-
-
ophio-amino-acid oxidase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidative deamination
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SYSTEMATIC NAME
IUBMB Comments
L-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9000-89-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-isoleucine + H2O + O2
3-methyl-2-oxo-pentanoate + NH3 + H2O2
show the reaction diagram
about 70% of the activity with L-leucine
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-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-methionine + H2O + O2
4-(methylsulfanyl)-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
about 110% of the activity with L-leucine
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-
?
L-phenylalanine + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
about 80% of the activity with L-leucine
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-
?
L-tyrosine + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
about 60% of the activity with L-leucine
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-
?
additional information
?
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
OXLA_BUNMU
517
0
58811
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 65000, SDS-PAGE, x * 58800, calculated from sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
sugars account for approximately 12% of total mass
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 month, 93% residual activity
4°C, 1 month, 76% residual activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Sephadex G-75 gel filtration, Sephadex G-25 desalting, Q ion-exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
apotxin can selectively kill tumor cells, with less cytotoxicity to the normal cells. Its anti-tumor activity is mainly due to the hydrogen peroxide produced from LAAO oxidation, but catalase does not reverse its anti-tumor effect completely. Deglycosylation can significantly reduce the LAAO activity and anti-tumor activity of apotxin
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jin, Y.; Lee, W.H.; Zeng, L.; Zhang, Y.
Molecular characterization of L-amino acid oxidase from king cobra venom
Toxicon
50
479-489
2007
Bungarus fasciatus (A8QL52), Bungarus fasciatus, Bungarus multicinctus (A8QL51), Bungarus multicinctus, Naja atra (A8QL58), Naja atra, Ophiophagus hannah (P81383), Ophiophagus hannah
Manually annotated by BRENDA team
Lu, W.; Hu, L.; Yang, J.; Sun, X.; Yan, H.; Liu, J.; Chen, J.; Cheng, X.; Zhou, Q.; Yu, Y.; Wei, J.F.; Cao, P.
Isolation and pharmacological characterization of a new cytotoxic L-amino acid oxidase from Bungarus multicinctus snake venom
J. Ethnopharmacol.
213
311-320
2018
Bungarus multicinctus (A8QL51), Bungarus multicinctus
Manually annotated by BRENDA team