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Information on EC 1.4.1.4 - glutamate dehydrogenase (NADP+) and Organism(s) Chlorella sorokiniana and UniProt Accession P28998

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This record set is specific for:
Chlorella sorokiniana
UNIPROT: P28998 not found.
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The taxonomic range for the selected organisms is: Chlorella sorokiniana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nadp-gdh, nadp-dependent glutamate dehydrogenase, nadp-glutamate dehydrogenase, nadp-specific glutamate dehydrogenase, nadp-linked glutamate dehydrogenase, nadph-dependent glutamate dehydrogenase, glutamate dehydrogenase (nadp+), nadp+-dependent glutamate dehydrogenase, nadp+-dependent gdh, nadp-dependent gdh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate)
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glutamic acid dehydrogenase
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glutamic dehydrogenase
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L-glutamate dehydrogenase
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NAD(P)H-dependent glutamate dehydrogenase
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NADP-GDH
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NADP-specific glutamate dehydrogenase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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oxidative deamination
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reductive amination
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SYSTEMATIC NAME
IUBMB Comments
L-glutamate:NADP+ oxidoreductase (deaminating)
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CAS REGISTRY NUMBER
COMMENTARY hide
9029-11-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + NADPH + NH3
L-glutamate + NADP+ + H2O
show the reaction diagram
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?
2-oxoglutarate + NH4+ + NADPH
L-glutamate + NADP+
show the reaction diagram
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?
L-glutamate + NADP+ + H2O
2-oxoglutarate + NADPH + NH3
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamate + NADP+ + H2O
2-oxoglutarate + NADPH + NH3
show the reaction diagram
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.2 - 6.8
2-oxoglutarate
21 - 38.2
L-glutamate
0.015 - 0.04
NADP+
0.125 - 0.26
NADPH
3.3 - 80
NH4+
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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alpha-hexamer and beta-hexamer
65
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DELTA40N-homohexamer
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 70
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50°C: alpha-homohexamer shows about 65% of maximal activity, beta-homohexamer shows about shows about 70% of maximal activity, DELTA40N-homohexamer shows about 55% of maximal activity. 70°C: alpha-homohexamer shows about 90% of maximal activity, beta-homohexamer shows about 60% of maximal activity, DELTA40N-homohexamer shows about 80% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHE4_CHLSO
523
0
57530
Swiss-Prot
Chloroplast (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
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x * 53000, SDS-PAGE, beta-holoenzyme
55500
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x * 55500, SDS-PAGE, alpha-holoenzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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stable at
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purification under anoxically conditions, 2 mM dithiothreitol stabilizes
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus expressed in Escherichia coli
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length cDNA encoding alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus, DELTA40N, expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bascomb, N.F.; Schmidt, R.R.
Purification and partial kinetic and physical characterization of two chloroplast-localized NADP-specific glutamate dehydrogenase isoenzymes and their preferential accumulation in Chlorella sorokiniana cells cultured at low or high ammonium levels
Plant Physiol.
83
75-84
1987
Chlorella sorokiniana
Manually annotated by BRENDA team
Lawit, S.J.; Miller, P.W.; Dunn, W.I.; Mirabile, J.S.; Schmidt, R.R.
Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers
Plant Mol. Biol.
52
605-616
2003
Chlorella sorokiniana
Manually annotated by BRENDA team
Jaspard, E.
A computational analysis of the three isoforms of glutamate dehydrogenase reveals structural features of the isoform EC 1.4.1.4 supporting a key role in ammonium assimilation by plants
Biol. Direct
1
38
2006
Chlorella sorokiniana (P28998), Chlorella sorokiniana
Manually annotated by BRENDA team