50°C: alpha-homohexamer shows about 65% of maximal activity, beta-homohexamer shows about shows about 70% of maximal activity, DELTA40N-homohexamer shows about 55% of maximal activity. 70°C: alpha-homohexamer shows about 90% of maximal activity, beta-homohexamer shows about 60% of maximal activity, DELTA40N-homohexamer shows about 80% of maximal activity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length cDNA encoding alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus, DELTA40N, expression in Escherichia coli
lack of a structure called antenna, NAD(P)-binding motif GAGNVA, and a second putative coenzyme-binding motif GVLTGKG together with the four residues Lys, Ser, Arg and Thr involved in the binding of the reduced form of NADP, key role of GDH4 in ammonium assimilation
lack of a structure called antenna, NAD(P)-binding motif GAGNVA, and a second putative coenzyme-binding motif GVLTGKG together with the four residues Lys, Ser, Arg and Thr involved in the binding of the reduced form of NADP, key role of GDH4 in ammonium assimilation
Purification and partial kinetic and physical characterization of two chloroplast-localized NADP-specific glutamate dehydrogenase isoenzymes and their preferential accumulation in Chlorella sorokiniana cells cultured at low or high ammonium levels
Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers
A computational analysis of the three isoforms of glutamate dehydrogenase reveals structural features of the isoform EC 1.4.1.4 supporting a key role in ammonium assimilation by plants