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Information on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+] and Organism(s) Rattus norvegicus and UniProt Accession P10860

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Rattus norvegicus
UNIPROT: P10860 not found.
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hgdh2, glutamate dehydrogenase 1, gdhii, nad(p)-dependent glutamate dehydrogenase, legdh1, nad(p)+-dependent glutamate dehydrogenase, nad(p)-glutamate dehydrogenase, nad(p)h-dependent glutamate dehydrogenase, ttgdh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutamate dehydrogenase
-
glutamate dehydrogenase 1
-
dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate))
-
-
-
-
GDH
-
-
-
-
glutamic acid dehydrogenase
-
-
-
-
glutamic dehydrogenase
-
-
-
-
L-glutamate dehydrogenase
-
-
-
-
L-glutamic acid dehydrogenase
-
-
-
-
Legdh1
-
-
-
-
Membrane protein 50
-
-
-
-
MP50
-
-
-
-
NAD(P)-glutamate dehydrogenase
-
-
-
-
NAD(P)H-dependent glutamate dehydrogenase
-
-
-
-
NAD(P)H-utilizing glutamate dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
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oxidation
-
-
-
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reduction
-
-
-
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reductive amination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamate:NAD(P)+ oxidoreductase (deaminating)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9029-12-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamate + H2O + NAD+
2-oxoglutarate + NH3 + NADH + H+
show the reaction diagram
-
-
-
?
L-glutamate + H2O + NADP+
2-oxoglutarate + NH3 + NADPH + H+
show the reaction diagram
-
-
-
?
L-glutamate + NAD+ + H2O
2-oxoglutarate + NADH + NH3
show the reaction diagram
-
-
-
r
2-oxoglutarate + NADH + NH3
L-glutamate + NAD+ + H2O
show the reaction diagram
-
-
-
-
?
alanine + H2O + NAD(P)+
pyruvate + NH3 + NAD(P)H
show the reaction diagram
-
very low activity
-
-
?
L-glutamate + H2O + NAD(P)+
2-oxoglutarate + NH3 + NAD(P)H
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
cofactor specificity not mentioned
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GDP
strong allosteric inhibitor of GDH1 leading to a 90% reduction of activity. Addition of increasing concentrations of pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) leads to an increasing protection from GDP inhibition
Glyoxal
67% inhibition at 1 mM
methylglyoxal
with 1 mM methylglyoxal, GDH activity significantly decreases at 30 min of incubation, and markedly drops by 37% within 5 h compared to control
sulfite
-
uncompetitive
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
leucine
PMP-BCATm
pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) accelerates the oxidative deamination reaction of GDH1in the presence of branched-chain amino acids (Leu, Ile, Val). Reductive amination reaction is not affected
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.61 - 23.8
glutamate
0.364 - 0.924
NAD+
0.443 - 0.637
NADP+
additional information
additional information
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00005 - 0.00806
GTP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17.8
-
liver enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
reductive amination at
8
oxidative deamination at
8
-
reductive amination
9 - 9.5
-
glutamte deamination
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
half activity of glutamate deamination compared to pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.05
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
peripheral membrane protein
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
GDH1 is the protein partner for pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm). Facilitating the recycling of BCATm to form metabolon, GDH1 acts as a catalytic machine
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DHE3_RAT
558
0
61416
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61400
gel filtration
300000 - 350000
-
liver, sedimentation equilibrium
56000
-
x * 56000, enzymes from mitochondria and endoplasmic reticulum
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
method of determination not further specified
?
-
x * 56000, enzymes from mitochondria and endoplasmic reticulum
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
-
mitochondrial enzyme loses 80% activity after 20 min, enzyme from endoplasmic reticulum loses 20% activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose, ATP-agarose, Resource Q
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
complete loss of activity after incubation with 6 M guanidine hydrochloride or 7 M urea, no renaturation after dilution into 200 mM phosphate buffer
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
restricted feeding with food access for 2 h each day for three weeks promotes higher levels of mitochondrial glutamate dehydrogenase protein and activity, as well as a loss of 24-h rhythmicity, in comparison to ad libitum conditions. The rhythmicity of glutamate dehydrogenase activity detected in serum is changed
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Smith, E.L.; Austen, B.M.; Blumenthal, K.M.; Nyc, J.F.
Glutamate dehydrogenase
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
11
293-367
1975
Acholeplasma laidlawii, Auxenochlorella pyrenoidosa, Bos taurus, Gallus gallus, Homo sapiens, Lithobates catesbeianus, Rattus norvegicus, Squalus acanthias, Sus scrofa, Thunnus thynnus
-
Manually annotated by BRENDA team
Arnold, H.; Maier, K.P.
Glutamate dehydrogenase from rat liver
Biochim. Biophys. Acta
251
133-140
1971
Rattus norvegicus
Manually annotated by BRENDA team
Graham, L.D.; Griffin, T.O.; Beatty, R.E.; McCarthy, A.D.; Tipton, K.F.
Purification of liver glutamate dehydrogenase by affinity precipitation and studies on its denaturation
Biochim. Biophys. Acta
828
266-269
1985
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Rajas, F.; Rousset, B.
A membrane-bound form of glutamate dehydrogenase possesses an ATP-dependent high-affinity microtubule-binding activity
Biochem. J.
295
447-455
1993
Bos taurus, Rattus norvegicus, Sus scrofa
-
Manually annotated by BRENDA team
Lee, W.K.; Shin, S.; Cho, S.S.; Park, J.S.
Purification and characterization of glutamate dehydrogenase as another isoprotein binding to the membrane of rough endoplasmic reticulum
J. Cell. Biochem.
76
244-253
1999
Rattus norvegicus
Manually annotated by BRENDA team
Zhang, X.; Vincent, A.S.; Halliwell, B.; Wong, K.P.
A mechanism of sulfite neurotoxicity: direct inhibition of glutamate dehydrogenase
J. Biol. Chem.
279
43035-43045
2004
Rattus norvegicus
Manually annotated by BRENDA team
Hamelin, M.; Mary, J.; Vostry, M.; Friguet, B.; Bakala, H.
Glycation damage targets glutamate dehydrogenase in the rat liver mitochondrial matrix during aging
FEBS J.
274
5949-5961
2007
Rattus norvegicus (P10860)
Manually annotated by BRENDA team
Islam, M.M.; Nautiyal, M.; Wynn, R.M.; Mobley, J.A.; Chuang, D.T.; Hutson, S.M.
The branched chain amino acid (BCAA) metabolon: Interaction of glutamate dehydrogenase with the mitochondrial branched chain aminotransferase (BCATm)
J. Biol. Chem.
285
265-276
2010
Rattus norvegicus (P10860)
Manually annotated by BRENDA team
Vazquez-Martinez, O.; Mendez, I.; Turrubiate, I.; Valente-Godinez, H.; Perez-Mendoza, M.; Garcia-Tejada, P.; Diaz-Munoz, M.
Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location
Exp. Biol. Med. (Maywood)
242
945-952
2017
Rattus norvegicus (P10860)
Manually annotated by BRENDA team