Information on EC 1.4.1.28 - secondary-alkyl amine dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.4.1.28

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EC Tree 1 Oxidoreductases 1.4 Acting on the CH-NH₂ group of donors 1.4.1 With NAD⁺ or NADP⁺ as acceptor 1.4.1.28 secondary-alkyl amine dehydrogenase [NAD(P)+]

IUBMB Comments

The enzyme has been shown to react preferentially with short-chain ketones such as cyclohexanone, primary amine groups attached to secondary alkyl groups, or D- and L-amino acids. It also reduces aldehydes to primary amines. Cosubstrate preference depends on the substrate.

The enzyme appears in viruses and cellular organisms

Reaction Schemes

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a secondary-alkyl amine

Synonyms
AMDH, AmDH4, amine dehydrogenase, D187_009359, DapB_1, NAD+-dependent amine dehydrogenase, Pmob_1166, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

AMDH

AmDH4

amine dehydrogenase

D187_009359

DapB_1

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

NAD+-dependent amine dehydrogenase

2 entries

Pmob_1166

2 entries

AMDH

ambiguous

AMDH

Cystobacter fuscus

S9Q235

765439

AMDH

Cystobacter fuscus DSM 2262

S9Q235

AMDH

Laribacter hongkongensis

762848

AMDH

Microbacterium sp. MA1

C3UMY1

765439

AMDH

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

AMDH

Streptomyces virginiae

763366

AMDH

Streptomyces virginiae IFO 12827

AmDH4

AmDH4

AmDH4

Petrotoga mobilis DSM 10674

A9BHL2

amine dehydrogenase

ambiguous

amine dehydrogenase

Petrotoga mobilis

762878

amine dehydrogenase

Petrotoga mobilis DSM 10674

D187_009359

Cystobacter fuscus

S9Q235

763489

D187_009359

Cystobacter fuscus DSM 2262

S9Q235

D187_009359

Microbacterium sp. MA1

C3UMY1

763489

NAD+-dependent amine dehydrogenase

Streptomyces virginiae

763366

NAD+-dependent amine dehydrogenase

Streptomyces virginiae IFO 12827

Pmob_1166

Petrotoga mobilis

A9BHL2

763489

Pmob_1166

Petrotoga mobilis DSM 10674

A9BHL2

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

a secondary-alkyl amine + H2O + NAD(P)+ = a ketone + NH3 + NAD(P)H + H+

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SYSTEMATIC NAME

IUBMB Comments

secondary-alkyl amine:NAD(P)+ oxidoreductase (deaminating)

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

2 entries

(R)-1-phenylethan-1-amine + NAD+ + H2O

acetophenone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(R)-2-aminoheptane + NAD+ + H2O

2-oxoheptane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(R)-2-butylamine + NAD+ + H2O

2-oxobutane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(R,S)-2-amino-1-pentanol + NAD+ + H2O

1-hydroxypentan-2-one + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(R,S)-2-aminopentan-1-ol + H2O + NAD+

2-oxopentan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-1-phenylethan-1-amine + H2O + NAD+

acetophenone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-amino-1-butanol + NAD+ + H2O

1-hydroxybutan-2-one + NH3 + NADH + H+

2 entries

(S)-2-amino-3-methyl-1-butanol + NAD+ + H2O

1-hydroxy-3-methylbutan-2-one + NH3 + NADH + H+

2 entries

(S)-2-amino-3-methylbutan-1-ol + H2O + NAD+

3-methyl-2-oxobutan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-aminobutan-1-ol + H2O + NAD+

2-oxobutan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-aminoheptane + NAD+ + H2O

2-oxoheptane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-butylamine + H2O + NAD+

2-oxobutane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-butylamine + NAD+ + H2O

2-oxobutane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-isoleucinol + H2O + NAD+

? + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-isoleucinol + NAD+ + H2O

2-oxo-3-methylpentan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-leucinol + H2O + NAD+

? + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-leucinol + NAD+ + H2O

2-oxo-5-methylpentan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-methioninol + H2O + NAD+

? + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-methioninol + NAD+ + H2O

2-oxo-3-methylpentan-1-ol + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

1-cyclohexylethan-1-one + NH3 + NADH + H+

1-cyclohexylethyl-1-amine + NAD+ + H2O

3 entries

1-hydroxy-4-methylpentan-2-one + NH3 + NADH + H+

2-amino-4-methylpentan-1-ol + H2O + NAD+

Geobacillus stearothermophilus

P13154

764412

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

5 entries

1-hydroxyhexan-2-one + NH3 + NADH + H+

(S)-2-aminohexan-1-ol + H2O + NAD+

Geobacillus stearothermophilus

P13154

764412

Substrates: -
Products: -

1-indanone + NH3 + NADH + H+

1-indanamine + NAD+ + H2O

3 entries

2-amino-1-propanol + H2O + NAD+

1-hydroxyacetone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

2-aminocyclohexanol + NAD+ + H2O

2-hydroxycyclohexan-1-one + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

2-aminopentane + H2O + NAD+

2-oxopentane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

2-aminopentane + NAD+ + H2O

2-oxopentane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + H2O + NAD+

3 entries

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + NAD+ + H2O

3 entries

2-methylbutanal + NH3 + NADH + H+

2-methylbutan-1-amine + NAD+ + H2O

3 entries

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + H2O + NAD+

4 entries

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + NAD+ + H2O

4 entries

2-methylcyclopentanone + NH3 + NADH + H+

2-methylcyclopentylamine + NAD+ + H2O

3 entries

2-oxopropanoate + NH3 + NADH + H+

D,L-alanine + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

3,5-dimethylcyclohexanone + NH3 + NADH + H+

3,5-dimethylcyclohexylamine + NAD+ + H2O

3 entries

3-acetyl-1-propanol + NH3 + NADH + H+

3-aminobutan-1-ol + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

3-aminopentane + H2O + NAD+

3-oxopentane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

3-aminopentane + NAD+ + H2O

3-oxopentane + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

3-buten-2-one + NH3 + NADH + H+

3-buten-2-amine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

3-hydroxy-2-butanone + NH3 + NADH + H+

3-aminobutan-2-ol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

3-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-2-ol + NAD+ + H2O

3 entries

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + H2O + NAD+

3 entries

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + NAD+ + H2O

3 entries

3-methylbutanal + NH3 + NADH + H+

3-methylbutan-1-amine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: 214% of the activity with cyclohexanone
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + H2O + NAD+

4 entries

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + NAD+ + H2O

4 entries

3-methylcyclopentanone + NH3 + NADH + H+

3-methylcyclopentylamine + NAD+ + H2O

3 entries

4-hydroxy-2-butanone + NH3 + NADH + H+

3-aminobutan-1-ol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

4-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-1-ol + NAD+ + H2O

3 entries

4-hydroxyhexan-3-one + NH3 + NADH + H+

4-aminohexan-3-ol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + H2O + NAD+

4 entries

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + NAD+ + H2O

4 entries

4-oxopentanoate + NH3 + NADH + H+

(4S)-4-aminopentanoate + NAD+ + H2O

2 entries

4-oxopentanoate + NH3 + NADPH + H+

(4S)-4-aminopentanoate + H2O + NADP+

2 entries

4-phenylbutan-2-one + NH3 + NADH + H+

4-phenylbutan-2-amine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

5-hydroxyoctan-4-one + NH3 + NADH + H+

5-aminooctan-4-ol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

5-hydroxypentan-2-one + NH3 + NADH + H+

5-aminopentan-1-ol + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + H2O + NAD+

2 entries

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + NAD+ + H2O

2 entries

acetaldehyde + NH3 + NADH + H+

ethylamine + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

acetaldehyde + NH3 + NADH + H+

ethylamine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

acetophenone + NH3 + NADH + H+

(S)-alpha-methylbenzylamine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: -
Products: -

acetophenone + NH3 + NADH + H+

1-phenylethan-1-amine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

aspartate + H2O + NAD+

? + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

benzaldehyde + NH3 + NAD(P)H + H+

benzylamine + H2O + NAD(P)+

Laribacter hongkongensis

762848

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + H2O + NAD+

2 entries

benzaldehyde + NH3 + NADH + H+

benzylamine + NAD+ + H2O

4 entries

benzylamine + H2O + NAD+

benzaldehyde + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

benzylamine + NAD+ + H2O

benzaldehyde + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

but-3-en-2-one + NH3 + NADH + H+

but-3-en-2-amine + NAD+ + H2O

3 entries

butan-2-one + NH3 + NADH + H+

2-aminobutane + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

butan-2-one + NH3 + NADH + H+

2-aminobutane + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

butan-2-one + NH3 + NADH + H+

2-butylamine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: 5.5% of the activity with cyclohexanone
Products: -

butanal + NH3 + NADH + H+

butylamine + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

butanal + NH3 + NADH + H+

n-butylamine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

cyclohexanone + methylamine + NADH + H+

N-methylcyclohexylamine + NAD+ + H2O

4 entries

cyclohexanone + methylamine + NADPH + H+

N-methylcyclohexanamine + H2O + NADP+

4 entries

cyclohexanone + NH3 + NAD(P)H + H+

cyclohexylamine + H2O + NAD(P)+

Laribacter hongkongensis

762848

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + NAD+ + H2O

4 entries

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

5 entries

cyclopentanone + NH3 + NADPH + H+

cyclopentylamine + NADP+ + H2O

3 entries

D-alanine + H2O + NAD+

2-oxopropanoate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

D-alanine + NAD+ + H2O

2-oxopropanoate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

D-aspartate + H2O + NAD+

2-oxobutanedioate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

D-glutamate + H2O + NAD+

2-oxopentanedioate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

D-glutamate + NAD+ + H2O

2-oxopentanedioic acid + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

D-serine + NAD+ + H2O

3-hydroxy-2-oxopropanoate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

dihydroxyacetone + NH3 + NADH + H+

serinol + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

dihydroxyacetone + NH3 + NADH + H+

serinol + NAD+ + H2O

2 entries

ethylamine + NAD+ + H2O

acetaldehyde + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

hexan-2-one + NH3 + NADH + H+

2-aminohexane + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

hexan-3-one + NH3 + NADH + H+

hexan-3-amine + NAD+ + H2O

3 entries

hexanal + NH3 + NADH + H+

n-hexylamine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

hydroxyacetone + NH3 + NADH + H+

1-aminopropanone + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

hydroxyacetone + NH3 + NADH + H+

2-aminopropan-1-ol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

isobutanal + methylamine + NADH + H+

N,2-dimethylpropan-1-amine + NAD+ + H2O

3 entries

isobutanal + NH3 + NADH + H+

2-methylpropan-1-amine + NAD+ + H2O

3 entries

isovaleraldehyde + NH3 + NAD(P)H + H+

3-methylbutan-1-amine + H2O + NAD(P)+

Laribacter hongkongensis

762848

Substrates: -
Products: -

L-alanine + H2O + NAD+

2-oxopropanoate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-alanine + NAD+ + H2O

2-oxopropanoate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-aspartate + H2O + NAD+

2-oxobutanedioate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-aspartate + NAD+ + H2O

2-oxobutanedioic acid + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-glutamate + H2O + NAD+

2-oxopentanedioate + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-glutamate + NAD+ + H2O

2-oxopentanedioic acid + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

L-serine + NAD+ + H2O

2-oxopentanedioic acid + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

methylamin + NAD+ + H2O

formaldehyde + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

n-butylamine + NAD+ + H2O

butanal + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

n-hexylamine + NAD+ + H2O

hexanal + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

n-octylamine + NAD+ + H2O

octanal + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

n-propylamine + NAD+ + H2O

propanal + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

2-aminopentane + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

2-aminopentane + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

5 entries

pentan-3-one + NH3 + NADH + H+

pentan-3-amine + NAD+ + H2O

3 entries

pentanal + NH3 + NADH + H+

n-pentylamine + NAD+ + H2O

3 entries

phenethylamine + H2O + NAD+

acetophenone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

propionaldehyde + NH3 + NADH + H+

n-propylamine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

serinol + H2O + NAD+

dihydroxyacetone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

serinol + NAD+ + H2O

dihydroxyacetone + NH3 + NADH + H+

2 entries

additional information

9 entries

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

Petrotoga mobilis

A9BHL2

763489

Substrates: -
Products: -

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

Petrotoga mobilis DSM 10674

A9BHL2

763489

Substrates: -
Products: -

(S)-2-amino-1-butanol + NAD+ + H2O

1-hydroxybutan-2-one + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-amino-1-butanol + NAD+ + H2O

1-hydroxybutan-2-one + NH3 + NADH + H+

Streptomyces virginiae IFO 12827

763366

Substrates: -
Products: -

(S)-2-amino-3-methyl-1-butanol + NAD+ + H2O

1-hydroxy-3-methylbutan-2-one + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

(S)-2-amino-3-methyl-1-butanol + NAD+ + H2O

1-hydroxy-3-methylbutan-2-one + NH3 + NADH + H+

Streptomyces virginiae IFO 12827

763366

Substrates: -
Products: -

1-cyclohexylethan-1-one + NH3 + NADH + H+

1-cyclohexylethyl-1-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

1-cyclohexylethan-1-one + NH3 + NADH + H+

1-cyclohexylethyl-1-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

1-cyclohexylethan-1-one + NH3 + NADH + H+

1-cyclohexylethyl-1-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

Geobacillus stearothermophilus

P13154

764412

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

Lysinibacillus sphaericus

W7S058

764412

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

Lysinibacillus sphaericus CBAM5

W7S058

764412

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

Sporosarcina psychrophila

I0IJU1

764412

Substrates: -
Products: -

1-hydroxybutan-2-one + NH3 + NAD(P)H + H+

(S)-2-aminobutan-1-ol + H2O + NAD(P)+

Thermoactinomyces intermedius

Q60030

764412

Substrates: -
Products: -

1-indanone + NH3 + NADH + H+

1-indanamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

1-indanone + NH3 + NADH + H+

1-indanamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

1-indanone + NH3 + NADH + H+

1-indanamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

2-cyclohexen-1-one + NH3 + NADH + H+

2-cyclohexen-1-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

2-methylbutanal + NH3 + NADH + H+

2-methylbutan-1-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

2-methylbutanal + NH3 + NADH + H+

2-methylbutan-1-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

2-methylbutanal + NH3 + NADH + H+

2-methylbutan-1-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus DSM 2262

S9Q235

765439

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus DSM 2262

S9Q235

763489

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

2-methylcyclohexanone + NH3 + NADH + H+

2-methylcyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

2-methylcyclopentanone + NH3 + NADH + H+

2-methylcyclopentylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

2-methylcyclopentanone + NH3 + NADH + H+

2-methylcyclopentylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

2-methylcyclopentanone + NH3 + NADH + H+

2-methylcyclopentylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

3,5-dimethylcyclohexanone + NH3 + NADH + H+

3,5-dimethylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

3,5-dimethylcyclohexanone + NH3 + NADH + H+

3,5-dimethylcyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

3,5-dimethylcyclohexanone + NH3 + NADH + H+

3,5-dimethylcyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

3-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-2-ol + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

3-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-2-ol + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

3-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-2-ol + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

3-methylbutan-2-one + NH3 + NADH + H+

3-methylbutan-2-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus DSM 2262

S9Q235

765439

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus DSM 2262

S9Q235

763489

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

3-methylcyclohexanone + NH3 + NADH + H+

3-methylcyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

3-methylcyclopentanone + NH3 + NADH + H+

3-methylcyclopentylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

3-methylcyclopentanone + NH3 + NADH + H+

3-methylcyclopentylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

3-methylcyclopentanone + NH3 + NADH + H+

3-methylcyclopentylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

4-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-1-ol + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

4-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-1-ol + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

4-hydroxybutan-2-one + NH3 + NADH + H+

3-aminobutan-1-ol + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + H2O + NAD+

Cystobacter fuscus DSM 2262

S9Q235

765439

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus DSM 2262

S9Q235

763489

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

4-methylcyclohexanone + NH3 + NADH + H+

4-methylcyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

4-oxopentanoate + NH3 + NADH + H+

(4S)-4-aminopentanoate + NAD+ + H2O

Petrotoga mobilis

762878

Substrates: -
Products: -

4-oxopentanoate + NH3 + NADH + H+

(4S)-4-aminopentanoate + NAD+ + H2O

Petrotoga mobilis DSM 10674

762878

Substrates: -
Products: -

4-oxopentanoate + NH3 + NADPH + H+

(4S)-4-aminopentanoate + H2O + NADP+

Petrotoga mobilis

762878

Substrates: -
Products: -

4-oxopentanoate + NH3 + NADPH + H+

(4S)-4-aminopentanoate + H2O + NADP+

Petrotoga mobilis DSM 10674

762878

Substrates: -
Products: -

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + H2O + NAD+

Petrotoga mobilis

762878

Substrates: -
Products: -

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + H2O + NAD+

Petrotoga mobilis DSM 10674

762878

Substrates: -
Products: -

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + NAD+ + H2O

Petrotoga mobilis

762878

Substrates: -
Products: -

5-oxohexanoate + NH3 + NADH + H+

(5S)-5-aminohexanoate + NAD+ + H2O

Petrotoga mobilis DSM 10674

762878

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: 10.4% of the activity with cyclohexanone
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + H2O + NAD+

Streptomyces virginiae

763366

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

benzaldehyde + NH3 + NADH + H+

benzylamine + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

but-3-en-2-one + NH3 + NADH + H+

but-3-en-2-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

but-3-en-2-one + NH3 + NADH + H+

but-3-en-2-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

but-3-en-2-one + NH3 + NADH + H+

but-3-en-2-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

cyclohexanone + methylamine + NADH + H+

N-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

cyclohexanone + methylamine + NADH + H+

N-methylcyclohexylamine + NAD+ + H2O

Cystobacter fuscus DSM 2262

S9Q235

763489

Substrates: -
Products: -

cyclohexanone + methylamine + NADH + H+

N-methylcyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

cyclohexanone + methylamine + NADH + H+

N-methylcyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

cyclohexanone + methylamine + NADPH + H+

N-methylcyclohexanamine + H2O + NADP+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

cyclohexanone + methylamine + NADPH + H+

N-methylcyclohexanamine + H2O + NADP+

Cystobacter fuscus DSM 2262

S9Q235

765439

Substrates: -
Products: -

cyclohexanone + methylamine + NADPH + H+

N-methylcyclohexanamine + H2O + NADP+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

cyclohexanone + methylamine + NADPH + H+

N-methylcyclohexanamine + H2O + NADP+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + NAD+ + H2O

Cystobacter fuscus DSM 2262

S9Q235

763489

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

cyclohexanone + NH3 + NADH + H+

cyclohexylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

Cystobacter fuscus

S9Q235

765439

Substrates: -
Products: -

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

Cystobacter fuscus DSM 2262

S9Q235

765439

Substrates: -
Products: -

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

Laribacter hongkongensis

762848

Substrates: 3.4% of the activity with cyclohexanone
Products: -

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

Microbacterium sp. MA1

C3UMY1

765439

Substrates: -
Products: -

cyclopentanone + NH3 + NADH + H+

cyclopentylamine + H2O + NAD+

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: -
Products: -

cyclopentanone + NH3 + NADPH + H+

cyclopentylamine + NADP+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

cyclopentanone + NH3 + NADPH + H+

cyclopentylamine + NADP+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

cyclopentanone + NH3 + NADPH + H+

cyclopentylamine + NADP+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

dihydroxyacetone + NH3 + NADH + H+

serinol + NAD+ + H2O

Streptomyces virginiae

763366

Substrates: -
Products: -

dihydroxyacetone + NH3 + NADH + H+

serinol + NAD+ + H2O

Streptomyces virginiae IFO 12827

763366

Substrates: -
Products: -

hexan-3-one + NH3 + NADH + H+

hexan-3-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

hexan-3-one + NH3 + NADH + H+

hexan-3-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

hexan-3-one + NH3 + NADH + H+

hexan-3-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

isobutanal + methylamine + NADH + H+

N,2-dimethylpropan-1-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

isobutanal + methylamine + NADH + H+

N,2-dimethylpropan-1-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

isobutanal + methylamine + NADH + H+

N,2-dimethylpropan-1-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

isobutanal + NH3 + NADH + H+

2-methylpropan-1-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

isobutanal + NH3 + NADH + H+

2-methylpropan-1-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

isobutanal + NH3 + NADH + H+

2-methylpropan-1-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Petrotoga mobilis

A9BHL2

763489

Substrates: no substrate of wild-type, but substrate of mutants carrying substitution R161M
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Petrotoga mobilis DSM 10674

A9BHL2

763489

Substrates: no substrate of wild-type, but substrate of mutants carrying substitution R161M
Products: -

pentan-3-one + NH3 + NADH + H+

pentan-3-amine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

pentan-3-one + NH3 + NADH + H+

pentan-3-amine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

pentan-3-one + NH3 + NADH + H+

pentan-3-amine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

pentanal + NH3 + NADH + H+

n-pentylamine + NAD+ + H2O

Cystobacter fuscus

S9Q235

763489

Substrates: -
Products: -

pentanal + NH3 + NADH + H+

n-pentylamine + NAD+ + H2O

Microbacterium sp. MA1

C3UMY1

763489

Substrates: -
Products: -

pentanal + NH3 + NADH + H+

n-pentylamine + NAD+ + H2O

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

Substrates: -
Products: -

serinol + NAD+ + H2O

dihydroxyacetone + NH3 + NADH + H+

Streptomyces virginiae

763366

Substrates: -
Products: -

serinol + NAD+ + H2O

dihydroxyacetone + NH3 + NADH + H+

Streptomyces virginiae IFO 12827

763366

Substrates: -
Products: -

additional information

Cystobacter fuscus

S9Q235

765439

Substrates: also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde
Products: -

additional information

Laribacter hongkongensis

762848

Substrates: poor substrates: linear aliphatic carbonyl compounds such as 3-heptanone, 2-pentanone as well as cyclopentanone
Products: -

additional information

Microbacterium sp. MA1

C3UMY1

765439

Substrates: also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde
Products: -

additional information

Mycolicibacterium smegmatis

A0A8B4R7U8

765439

Substrates: also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde
Products: -

additional information

Petrotoga mobilis

762878

Substrates: no substrates: acetophenone, cyclohexanone, penta-2-one or 2-aminopentan-2-one
Products: -

additional information

Petrotoga mobilis DSM 10674

762878

Substrates: no substrates: acetophenone, cyclohexanone, penta-2-one or 2-aminopentan-2-one
Products: -

additional information

Streptomyces virginiae

763366

Substrates: enzyme catalyzes the reversible oxidative deaminations of amines and also amino alcohols and amino acids
Products: -

additional information

Streptomyces virginiae

763366

Substrates: catalyzes the reversible oxidative deaminations of amines but also amino alcohols and amino acids
Products: -

additional information

Streptomyces virginiae IFO 12827

763366

Substrates: enzyme catalyzes the reversible oxidative deaminations of amines and also amino alcohols and amino acids
Products: -

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show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

2 entries

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

2 entries

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

Petrotoga mobilis

A9BHL2

763489

Substrates: -
Products: -

(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+

(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O

Petrotoga mobilis DSM 10674

A9BHL2

763489

Substrates: -
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Petrotoga mobilis

A9BHL2

763489

Substrates: no substrate of wild-type, but substrate of mutants carrying substitution R161M
Products: -

pentan-2-one + NH3 + NADH + H+

pentan-2-amine + NAD+ + H2O

Petrotoga mobilis DSM 10674

A9BHL2

763489

Substrates: no substrate of wild-type, but substrate of mutants carrying substitution R161M
Products: -

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NAD+

NADH

NADP+

NADPH

additional information

Streptomyces virginiae

enzyme does not require artificial electron acceptors such as phenazine methosulfate

763366

NAD+

Streptomyces virginiae

enzyme is dependent on NAD+ or NADH

763366

NAD+

Streptomyces virginiae

dependent on NAD+ or NADH

763366

NADH

Laribacter hongkongensis

762848

NADH

Streptomyces virginiae

enzyme is dependent on NAD+ or NADH

763366

NADH

Petrotoga mobilis

activity with NADH is 10 times higher than that with NADPH

762878

NADH

Mycolicibacterium smegmatis

A0A8B4R7U8

specific activities for both cofactors NADH and NADPH are substrate-dependent

763489

NADH

Cystobacter fuscus

S9Q235

specific activities for both cofactors NADH and NADPH are substrate-dependent

763489

NADH

Microbacterium sp. MA1

C3UMY1

specific activities for both cofactors NADH and NADPH are substrate-dependent

NADH

NADH

Streptomyces virginiae

dependent on NAD+ or NADH

763366

NADP+

Streptomyces virginiae

substrate serinol, 13% of the activity with NAD+

763366

NADP+

Streptomyces virginiae

about 13% of the activity with NAD+

763366

NADPH

Streptomyces virginiae

substrate dihydroxyacetone, 8.5% of the activity with NAD+

763366

NADPH

Petrotoga mobilis

activity with NADH is 10 times higher than that with NADPH

762878

NADPH

Mycolicibacterium smegmatis

A0A8B4R7U8

specific activities for both cofactors NADH and NADPH are substrate-dependent

763489

NADPH

Cystobacter fuscus

S9Q235

specific activities for both cofactors NADH and NADPH are substrate-dependent

763489

NADPH

Microbacterium sp. MA1

C3UMY1

specific activities for both cofactors NADH and NADPH are substrate-dependent

763489

NADPH

Streptomyces virginiae

about 8.5% of the activity with NADH

763366

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-methylcyclohexanone

cyclohexanone

Isobutanal

NH3

substrate inhibition

2-methylcyclohexanone

Microbacterium sp. MA1

C3UMY1

763489

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

763489

2-methylcyclohexanone

cyclohexanone

substrate inhibition

cyclohexanone

Microbacterium sp. MA1

C3UMY1

763489

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

cyclohexanone

Isobutanal

Microbacterium sp. MA1

C3UMY1

763489

Isobutanal

Mycolicibacterium smegmatis

A0A8B4R7U8

Isobutanal

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.326 - 0.894

(2R)-2-amino-4-oxopentanoate

2 entries

1.48 - 3.64

1-hydroxybutan-2-one

5 entries

2.32

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.55 - 2.5

cyclohexanone

3 entries

2.2

dihydroxyacetone

2 entries

0.54 - 0.86

Isobutanal

2 entries

0.84

NAD+

2 entries

0.022 - 0.29

NADH

5 entries

0.01 - 0.06

NADPH

2 entries

26.5 - 392.2

NH3

7 entries

serinol

2 entries

0.326

(2R)-2-amino-4-oxopentanoate

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

0.894

(2R)-2-amino-4-oxopentanoate

Petrotoga mobilis

A9BHL2

mutant E102A, pH 9.5, 23°C

763489

1.48

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 8.5, 50°C

764412

2.32

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 9.0, 55°C

764412

2.42

1-hydroxybutan-2-one

Sporosarcina psychrophila

I0IJU1

pH 8.5, 50°C

764412

3.44

1-hydroxybutan-2-one

Lysinibacillus sphaericus

W7S058

pH 8.5, 55°C

764412

3.64

1-hydroxybutan-2-one

Thermoactinomyces intermedius

Q60030

pH 8.5, 45°C

764412

0.55

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

2.47

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

2.5

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

765439

2.2

dihydroxyacetone

Streptomyces virginiae

pH 7, 25°C

763366

2.2

dihydroxyacetone

Streptomyces virginiae

pH 9, 25°C

763366

0.54

Isobutanal

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

0.86

Isobutanal

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

0.84

NAD+

Streptomyces virginiae

pH 9, 25°C

763366

0.84

NAD+

Streptomyces virginiae

pH 8, 25°C

763366

0.022

NADH

Streptomyces virginiae

pH 7, 25°C

763366

0.022

NADH

Streptomyces virginiae

pH 9, 25°C

763366

0.025

NADH

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

0.06

NADH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489, 765439

0.29

NADH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.01

NADPH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489, 765439

0.06

NADPH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

26.5

NH3

Streptomyces virginiae

pH 7, 25°C

763366

26.5

NH3

Streptomyces virginiae

pH 9, 25°C

763366

98.9

NH3

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

765439

98.94

NH3

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

319.7

NH3

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

392

NH3

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

765439

392.2

NH3

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

serinol

Streptomyces virginiae

pH 9, 25°C

763366

serinol

Streptomyces virginiae

pH 8, 25°C

763366

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.5

(2R)-2-amino-4-oxopentanoate

Petrotoga mobilis

A9BHL2

mutant E102A, pH 9.5, 23°C

763489

0.32 - 1.79

1-hydroxybutan-2-one

5 entries

0.61

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.13 - 13

cyclohexanone

3 entries

0.11 - 0.54

Isobutanal

2 entries

0.1 - 0.58

NADH

2 entries

0.05 - 0.28

NADPH

2 entries

0.04 - 37.8

NH3

3 entries

0.32

1-hydroxybutan-2-one

Thermoactinomyces intermedius

Q60030

pH 8.5, 45°C

764412

0.74

1-hydroxybutan-2-one

Sporosarcina psychrophila

I0IJU1

pH 8.5, 50°C

764412

0.77

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 8.5, 50°C

764412

1.52

1-hydroxybutan-2-one

Lysinibacillus sphaericus

W7S058

pH 8.5, 55°C

764412

1.79

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 9.0, 55°C

764412

0.13

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

0.94

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

cyclohexanone

pH 9.5, 23°C

0.11

Isobutanal

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

0.54

Isobutanal

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

0.1

NADH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489, 765439

0.58

NADH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.05

NADPH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489, 765439

0.28

NADPH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.04

NH3

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489, 765439

0.3

NH3

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

37.8

NH3

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.81 - 130

(2R)-2-amino-4-oxopentanoate

2 entries

0.09 - 0.71

1-hydroxybutan-2-one

5 entries

0.26 - 0.261

2-methylcyclohexanone

2 entries

0.054 - 54.4

cyclohexanone

4 entries

0.209 - 0.62

Isobutanal

2 entries

1.705 - 1704

NADH

5 entries

4.537 - 4537

NADPH

4 entries

0.0004 - 0.4

NH3

4 entries

2.81

(2R)-2-amino-4-oxopentanoate

Petrotoga mobilis

A9BHL2

mutant E102A, pH 9.5, 23°C

763489

130

(2R)-2-amino-4-oxopentanoate

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

0.09

1-hydroxybutan-2-one

Thermoactinomyces intermedius

Q60030

pH 8.5, 45°C

764412

0.31

1-hydroxybutan-2-one

Sporosarcina psychrophila

I0IJU1

pH 8.5, 50°C

764412

0.44

1-hydroxybutan-2-one

Lysinibacillus sphaericus

W7S058

pH 8.5, 55°C

764412

0.52

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 8.5, 50°C

764412

0.71

1-hydroxybutan-2-one

Geobacillus stearothermophilus

P13154

pH 9.0, 55°C

764412

0.26

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

0.261

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

765439

0.054

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

1.72

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

1.723

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

765439

54.4

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

765439

0.209

Isobutanal

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

0.62

Isobutanal

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

1.705

NADH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

1.99

NADH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

1.995

NADH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

765439

1440

NADH

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

1704

NADH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

765439

4.537

NADPH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

4.89

NADPH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

4.893

NADPH

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

765439

4537

NADPH

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

765439

0.0004

NH3

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

0.0008

NH3

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489, 765439

0.118

NH3

Petrotoga mobilis

A9BHL2

wild-type, pH 9.5, 23°C

763489

0.4

NH3

pH 9.5, 23°C

show all columns Go to Ki Value Search

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

4.94

2-methylcyclohexanone

3 entries

0.41 - 1.99

cyclohexanone

4 entries

2.76

Isobutanal

3 entries

10623

NH3

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

4.94

2-methylcyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

4.94

2-methylcyclohexanone

Microbacterium sp. MA1

C3UMY1

pH 9.5, 23°C

763489

4.94

2-methylcyclohexanone

Petrotoga mobilis

A9BHL2

pH 9.5, 23°C

763489

0.41

cyclohexanone

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

0.41

cyclohexanone

Microbacterium sp. MA1

C3UMY1

pH 9.5, 23°C

763489

0.41

cyclohexanone

Petrotoga mobilis

A9BHL2

pH 9.5, 23°C

763489

1.99

cyclohexanone

Cystobacter fuscus

S9Q235

pH 9.5, 23°C

763489

2.76

Isobutanal

Mycolicibacterium smegmatis

A0A8B4R7U8

pH 9.5, 23°C

763489

2.76

Isobutanal

Microbacterium sp. MA1

C3UMY1

pH 9.5, 23°C

763489

2.76

Isobutanal

pH 9.5, 23°C

show all columns Go to Specific Activity Search

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10.1

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C

763489

104.8

Petrotoga mobilis

A9BHL2

mutant N135V/N163V/R161M/H264L, substrate pentan-2-one, 23°C, pH 9.5

763489

109.6

2 entries

119.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

120.6

129

131.6

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

14.9

Microbacterium sp. MA1

C3UMY1

substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C

765439

144.5

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C

763489

159.4

Microbacterium sp. MA1

C3UMY1

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

16.4

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

160.9

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

161

Microbacterium sp. MA1

C3UMY1

substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

170

Laribacter hongkongensis

substrate cyclohexanone, pH not specified in the publication, temperature not specified in the publication

762848

170.1

Microbacterium sp. MA1

C3UMY1

substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

175.2

18.6

18.8

substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

19.6

196.2

2.3

2.7

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-buten-2-one, cosubstrate NADH, pH 9.5, 23°C

765439

206.5

Microbacterium sp. MA1

C3UMY1

substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

233.2

245.6

25.1

25.5

252.6

257.7

substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C

763489

3 - 8

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

3.6

Cystobacter fuscus

S9Q235

substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C

765439

33.8

Cystobacter fuscus

S9Q235

substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

337

2 entries

34.2

Microbacterium sp. MA1

C3UMY1

substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C

765439

Cystobacter fuscus

S9Q235

substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

364

Laribacter hongkongensis

substrate isovaleraldehyde, pH not specified in the publication, temperature not specified in the publication

762848

37.1

Microbacterium sp. MA1

C3UMY1

substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C

765439

37.3

2 entries

388.6

Microbacterium sp. MA1

C3UMY1

substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

389

Microbacterium sp. MA1

C3UMY1

substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

397.9

Cystobacter fuscus

S9Q235

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

4.3

Cystobacter fuscus

S9Q235

substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C

765439

40.1

Cystobacter fuscus

S9Q235

substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C

765439

41.5

Cystobacter fuscus

S9Q235

substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C

765439

5.6

2 entries

51.9

Petrotoga mobilis

substrate 4-oxopentanoate, pH 7.2, 25°C

762878

54.4

2 entries

60.1

Microbacterium sp. MA1

C3UMY1

substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

60.2

2 entries

60.6

Microbacterium sp. MA1

C3UMY1

substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C

765439

614

Microbacterium sp. MA1

C3UMY1

substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

614.5

Microbacterium sp. MA1

C3UMY1

substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C

763489

63.6

Cystobacter fuscus

S9Q235

substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

7.4

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C

765439

7.9

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-buten-2-one, cosubstrate NADPH, pH 9.5, 23°C

765439

73.8

Cystobacter fuscus

S9Q235

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

75.2

2 entries

85.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

851.3

Microbacterium sp. MA1

C3UMY1

substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C

763489

88.1

2 entries

9.3

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

90.4

Microbacterium sp. MA1

C3UMY1

substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C

763489

955

Cystobacter fuscus

S9Q235

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

97.6

Microbacterium sp. MA1

C3UMY1

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

109.6

Cystobacter fuscus

S9Q235

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

109.6

Cystobacter fuscus

S9Q235

substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C

765439

120.6

Cystobacter fuscus

S9Q235

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

120.6

Cystobacter fuscus

S9Q235

substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C

765439

129

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

129

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C

765439

175.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylcyclohexanone, cosubstrates NH3 andNADPH, pH 9.5, 23°C

763489

175.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

18.6

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

18.6

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C

765439

19.6

Cystobacter fuscus

S9Q235

substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

19.6

Cystobacter fuscus

S9Q235

substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

196.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C

763489

196.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

2.3

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C

765439

2.3

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C

765439

233.2

Cystobacter fuscus

S9Q235

substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C

763489

233.2

Cystobacter fuscus

S9Q235

substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

245.6

Microbacterium sp. MA1

C3UMY1

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

245.6

Microbacterium sp. MA1

C3UMY1

substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C

765439

25.1

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

25.1

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

25.5

Cystobacter fuscus

S9Q235

substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

25.5

Cystobacter fuscus

S9Q235

substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

252.6

Microbacterium sp. MA1

C3UMY1

substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

252.6

Microbacterium sp. MA1

C3UMY1

substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

337

Microbacterium sp. MA1

C3UMY1

substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

337

Microbacterium sp. MA1

C3UMY1

substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

37.3

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

37.3

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C

765439

5.6

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C

765439

5.6

Petrotoga mobilis

substrate 5-oxohexanoate, pH 7.2, 25°C

762878

54.4

Microbacterium sp. MA1

C3UMY1

substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

54.4

Microbacterium sp. MA1

C3UMY1

substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C

765439

60.2

Cystobacter fuscus

S9Q235

substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

60.2

Cystobacter fuscus

S9Q235

substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C

765439

75.2

Microbacterium sp. MA1

C3UMY1

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

75.2

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

88.1

Microbacterium sp. MA1

C3UMY1

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

88.1

Mycolicibacterium smegmatis

A0A8B4R7U8

substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C

763489

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Streptomyces virginiae

deaminating reaction

6.5 - 7

8.5

6.5 - 7

Streptomyces virginiae

amination reaction

763366

6.5 - 7

Streptomyces virginiae

aminating reaction

763366

8.5

Geobacillus stearothermophilus

P13154

764412

8.5

Lysinibacillus sphaericus

W7S058

764412

8.5

Sporosarcina psychrophila

I0IJU1

764412

8.5

Thermoactinomyces intermedius

Q60030

764412

Geobacillus stearothermophilus

P13154

764412

Laribacter hongkongensis

762848

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Streptomyces virginiae

763366

Thermoactinomyces intermedius

Q60030

764412

Geobacillus stearothermophilus

activity of 0.7 U per milligram of protein

762878

Geobacillus stearothermophilus

P13154

764412

Lysinibacillus sphaericus

W7S058

764412

Sporosarcina psychrophila

I0IJU1

764412

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Petrotoga mobilis

hardly active at 20°C

good activity

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Cystobacter fuscus

763489, 765439

S9Q235

UniProt

brenda

Cystobacter fuscus DSM 2262

763489, 765439

S9Q235

UniProt

brenda

Geobacillus stearothermophilus

2 entries

Laribacter hongkongensis

762848

brenda

Lysinibacillus sphaericus

cf. EC 1.4.1.9

764412

W7S058

UniProt

brenda

Lysinibacillus sphaericus CBAM5

cf. EC 1.4.1.9

764412

W7S058

UniProt

brenda

Microbacterium sp. MA1

763489, 765439

C3UMY1

UniProt

brenda

Mycolicibacterium smegmatis

763489, 765439

A0A8B4R7U8

UniProt

brenda

Petrotoga mobilis

2 entries

Petrotoga mobilis DSM 10674

2 entries

Sporosarcina psychrophila

cf. EC 1.4.1.9

764412

I0IJU1

UniProt

brenda

Streptomyces virginiae

763366

brenda

Streptomyces virginiae IFO 12827

763366

brenda

Thermoactinomyces intermedius

cf. EC 1.4.1.9

764412

Q60030

UniProt

brenda

Geobacillus stearothermophilus

764412

brenda

Geobacillus stearothermophilus

cf. EC 1.4.1.9

764412

P13154

UniProt

brenda

brenda

UniProt

brenda

Petrotoga mobilis DSM 10674

762878

brenda

Petrotoga mobilis DSM 10674

763489

A9BHL2

UniProt

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

soluble

2 entries

soluble

Streptomyces virginiae

763366

brenda

soluble

Streptomyces virginiae IFO 12827

brenda

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Filter by:

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

Laribacter hongkongensis

in the docking analysis, cyclohexanone is well-orientated with -5.4 kcal/mol of binding energy and 3.16 A distance from the side chain of E104, key residue for interacting ammonia and substrate

762848

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

A0A0L8M630 pBLAST

AMDH_STRVG

Streptomyces virginiae

360

38495

Swiss-Prot

Show Sequence

other Location (Reliability: 1)

A9BHL2 pBLAST

A9BHL2_PETMO

Petrotoga mobilis (strain DSM 10674 / SJ95)

338

36847

TrEMBL

Show Sequence

C3UMY1 pBLAST

C3UMY1_9MICO

Microbacterium sp. MA1

354

38728

TrEMBL

Show Sequence

I0IJU1 pBLAST

I0IJU1_SPOPS

Sporosarcina psychrophila

364

39345

TrEMBL

Show Sequence

Mitochondrion (Reliability: 3)

P13154 pBLAST

DHLE_GEOSE

Geobacillus stearothermophilus

367

40458

Swiss-Prot

Show Sequence

Q60030 pBLAST

DHLE_THEIN

Thermoactinomyces intermedius

366

40586

Swiss-Prot

Show Sequence

S9Q235 pBLAST

S9Q235_CYSF2

Cystobacter fuscus (strain ATCC 25194 / DSM 2262 / NBRC 100088 / M29)

342

36999

TrEMBL

Show Sequence

W7S058 pBLAST

W7S058_LYSSH

Lysinibacillus sphaericus CBAM5

364

39723

TrEMBL

Show Sequence

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

95000

Streptomyces virginiae

gel filtration

763366

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Laribacter hongkongensis

x * 36800, SDS-PAGE, recombinant protein

762848

dimer

2 entries

dimer

Streptomyces virginiae

2 * 46000, SDS-PAGE

763366

dimer

Streptomyces virginiae IFO 12827

2 * 46000, SDS-PAGE

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Go to Crystallization (commentary) Search

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

homology modeling of structure in complex with NADH. Residue S68 of AmDH forms a hydrogen bond with the substrate hydroxyl group, and there are also hydrophobic interactions between the substrate and the side chains of V116 and L261

Geobacillus stearothermophilus

P13154

764412

homology modeling of structure. The coordination of the amine group to E108 holds the tetrahedral carbon of the substrate bearing the amine

Laribacter hongkongensis

762848

structure without substrate but bound to the cofactor NAD+, and a model of the ternary complex with product (2R,4S)-2,4-diaminopentanoate in the closed conformation. The active site features residues Glu102, Val134, Asn135, Arg161, Asn163, Phe168, Val172, Gln176, His197, Ile198, His264, Gln266, Gly299 and Thr303

show all columns Go to Protein Variants Search

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E102A

Petrotoga mobilis

A9BHL2

residue Glu102 is involved in the activation of ammonia, mutation significantly decreases activity

763489

R161M

Petrotoga mobilis

A9BHL2

mutant gains activity with substrate pentan-2-one

763489

E102A

Petrotoga mobilis DSM 10674

residue Glu102 is involved in the activation of ammonia, mutation significantly decreases activity

R161M

Petrotoga mobilis DSM 10674

mutant gains activity with substrate pentan-2-one

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 entries

Streptomyces virginiae

30 min, pH 7, 0% residual activity

half-life 65 h

half-life 30 min

Streptomyces virginiae

30 min, pH 7, 55% residual activity

763366

Streptomyces virginiae

30 min, 55% residual ativity

763366

Streptomyces virginiae

30 min, pH 7, 25% residual activity

763366

Streptomyces virginiae

30 min, 25% residual ativity

763366

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

30°C, stable for several days

Petrotoga mobilis

762878

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

from cell-free extract by sequential column chromatography

Streptomyces virginiae

763366

from the cell-free extract by sequential column chromatography

Streptomyces virginiae

763366

purification by heat treatment without notable loss of material and activity

Petrotoga mobilis

762878

purified by heat treatment without notable loss of material and activity

Petrotoga mobilis

762878

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli

Laribacter hongkongensis

762848

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

3 entries

synthesis

Laribacter hongkongensis

reductive amination of cyclohexanone (up to 100 mM) into cyclohexylamine is performed with an AmDH and formate dehydrogenase system with more than 99% conversion using Escherichia coli whole cell as well as purified enzymes. Chiral amine are produced from the corresponding ketone using inexpensive ammonium formate as sole sacrificial agent and enzymes omega-transaminase, AmDH, and formate dehydrogenase

762848

synthesis

Laribacter hongkongensis

reductive amination of cyclohexanone (up to 100 mM) into cyclohexylamine using transgenic Escherichia coli as whole-cell reactors as well as purified enzymes

762848

synthesis

Geobacillus stearothermophilus

P13154

in the asymmetric amination of a series of structurally different hydroxy ketones, high stereoselectivity (above >99%) is achieved the reduction of aliphatic and aromatic compounds. The aliphatic substrates 1-hydroxy-4-methylpentan-2-one and 1-hydroxyhexan-2-one are aminated with 99% conversions

764412

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

762848

Lee, S.; Jeon, H.; Giri, P.; Lee, U.; Jung, H.; Lim, S.; Sarak, S.; Khobragade, T.; Kim, B.; Yun, H.

The reductive amination of carbonyl compounds using native amine dehydrogenase from Laribacter hongkongensis

Biotechnol. Bioprocess Eng.

384-391

2021

Laribacter hongkongensis

brenda

762878

Mayol, O.; David, S.; Darii, E.; Debard, A.; Mariage, A.; Pellouin, V.; Petit, J.-L.; Salanoubat, M.; de Berardinis, V.; Zaparucha, A.; Vergne-Vaxelaire, C.

Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis

Catal. Sci. Technol.

7421-7428

2016

Petrotoga mobilis, Petrotoga mobilis DSM 10674

brenda

763366

Itoh, N.; Yachi, C.; Kudome, T.

Determining a novel NAD+-dependent amine dehydrogenase with a broad substrate range from Streptomyces �irginiae IFO 12827 purification and characterization

J. Mol. Catal. B

281-290

2000

Streptomyces virginiae, Streptomyces virginiae IFO 12827

brenda

763489

Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.P.; Petit, J.-L.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.

A family of native amine dehydrogenases for the asymmetric reductive amination of ketones

Nat. Catal.

324

2019

Cystobacter fuscus (S9Q235), Cystobacter fuscus DSM 2262 (S9Q235), Microbacterium sp. MA1 (C3UMY1), Mycolicibacterium smegmatis (A0A8B4R7U8), Petrotoga mobilis (A9BHL2), Petrotoga mobilis DSM 10674 (A9BHL2)

brenda

764412

Wang,G.; Qu, G.; Li, J.K.; Ma, J.A.; Guo,J.; Miao, Y.; Sun, Z.

Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols

Catal. Sci. Technol.

5945-5952

2020

Geobacillus stearothermophilus, Geobacillus stearothermophilus (P13154), Lysinibacillus sphaericus (W7S058), Lysinibacillus sphaericus CBAM5 (W7S058), Sporosarcina psychrophila (I0IJU1), Thermoactinomyces intermedius (Q60030)

brenda

765439

Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.; Petit, J.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.

A family of native amine dehydrogenases for the asymmetric reductive amination of ketones

Nat. Catal.

324-333

2019

Cystobacter fuscus (S9Q235), Cystobacter fuscus DSM 2262 (S9Q235), Microbacterium sp. MA1 (C3UMY1), Mycolicibacterium smegmatis (A0A8B4R7U8)

brenda

Entries 1 - 20 of 6

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EXTERNAL LINKS (specific for EC-Number 1.4.1.28)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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