We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme from Pseudomonas sp. AK2 also acts on quinoline-8-carboxylate, whereas that from Serratia marcescens 2CC-1 will oxidize nicotinate; quinaldate is a substrate for both of these enzymes. 2,4,6-Trinitrobenzene sulfonate, 1,4-benzoquinone, 1,2-naphthoquinone, nitroblue tetrazolium, thionine and menadione will serve as an electron acceptor for the former enzyme and ferricyanide for the latter; Meldola blue, iodonitrotetrazolium chloride, phenazine methosulfate, 2,6-dichlorophenolindophenol and cytochrome c will act as electron acceptors for both.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
quinaldic acid 4-oxidoreductase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Oxidase, quinaldate 4-
-
-
-
-
Quinaldic acid 4-oxidoreductase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Quinaldate + acceptor + H2O = kynurenate + reduced acceptor
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
quinoline-2-carboxylate:acceptor 4-oxidoreductase (hydroxylating)
The enzyme from Pseudomonas sp. AK2 also acts on quinoline-8-carboxylate, whereas that from Serratia marcescens 2CC-1 will oxidize nicotinate; quinaldate is a substrate for both of these enzymes. 2,4,6-Trinitrobenzene sulfonate, 1,4-benzoquinone, 1,2-naphthoquinone, nitroblue tetrazolium, thionine and menadione will serve as an electron acceptor for the former enzyme and ferricyanide for the latter; Meldola blue, iodonitrotetrazolium chloride, phenazine methosulfate, 2,6-dichlorophenolindophenol and cytochrome c will act as electron acceptors for both.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
quinaldate + acceptor
kynurenate + reduced acceptor
quinoline 8-carboxylic acid + reduced acceptor
?
nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
-
-
-
?
nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
-
-
-
?
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
-
-
r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
-
-
r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
-
-
r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
-
-
r
Quinaldate + acceptor
?
-
inducible enzyme
-
-
?
Quinaldate + acceptor
?
-
inducible enzyme
-
-
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
i.e. quinoline 2-carboxylic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
i.e. quinoline 2-carboxylic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptor: K3[Fe(CN)6]
-
-
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptor: K3[Fe(CN)6]
-
-
?
quinaldate + acceptor
kynurenate + reduced acceptor
-
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
-
?
quinoline 8-carboxylic acid + reduced acceptor
?
-
-
-
-
?
quinoline 8-carboxylic acid + reduced acceptor
?
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Quinaldate + acceptor
?
-
inducible enzyme
-
-
?
Quinaldate + acceptor
?
-
inducible enzyme
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FAD
-
enzyme contains 1.8 mol of FAD per mol of enzyme
Pterin molybdenum cofactor
-
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Iron
-
contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Iron
-
enzyme contains 2.3-3.7 gatom of iron per mol of enzyme
Molybdenum
-
enzyme contains 1.6 g-atom per mol of enzyme
Molybdenum
-
enzyme contains 0.5-0.6 gatom of molybdenum per mol of enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cu2+
-
-
KCN
-
74% reactivation with S2-
methanol
-
-
Mn2+
-
-
NaAsO2
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.085 - 1.6
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
0.085
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
with quinaldate as cosubstrate
1.6
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
with quinaldate as cosubstrate
0.023
Quinaldate
-
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
1.45
Quinaldate
-
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
AK-2
-
-
brenda
AK-2
-
-
brenda
2CC-1
-
-
brenda
2CC-1
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
90000 - 100000
-
gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
dimer
-
1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
-
hexamer
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
hexamer
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
75
-
5 min, 32% loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-80°C, 8 days, 25% loss of activity
-
4°C, 8 days, 30% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Sauter, M.; Tshisuaka, B.; Fetzner, S.; Lingens, F.
XX. Quinaldic acid 4-oxidoreductase from Pseudomonas sp. AK-2 compared to other procaryotic molybdenum-containing hydroxylases
Biol. Chem. Hoppe-Seyler
374
1037-1046
1993
Pseudomonas sp., Pseudomonas sp. AK-2
brenda
Fetzner, S.; Lingens, F.
XVIII. Purification and some properties of the molybdenum- and iron-containing quinaldic acid 4-oxidoreductase from Serratia marcescens 2CC-1
Biol. Chem. Hoppe-Seyler
374
363-376
1993
Serratia marcescens, Serratia marcescens 2CC-1
brenda
Select items on the left to see more content.
html completed
1.3.99.18 quinaldate 4-oxidoreductase
more
top
