Information on EC 1.3.8.3 - (R)-benzylsuccinyl-CoA dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.8.3
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RECOMMENDED NAME
GeneOntology No.
(R)-benzylsuccinyl-CoA dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
toluene degradation to benzoyl-CoA (anaerobic)
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toluene degradation
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Toluene degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase
Requires FAD as prosthetic group. Unlike other acyl-CoA dehydrogenases, this enzyme exhibits high substrate- and enantiomer specificity; it is highly specific for (R)-benzylsuccinyl-CoA and is inhibited by (S)-benzylsuccinyl-CoA. Forms the third step in the anaerobic toluene metabolic pathway in Thauera aromatica. Ferricenium ion is an effective artificial electron acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
260966-98-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains T and M3, grown on toluene or m-xylene
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Manually annotated by BRENDA team
grown on toluene
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Manually annotated by BRENDA team
grown on toluene
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
show the reaction diagram
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate
(E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
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low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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no FMN or riboflavin detected
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(S)-benzylsuccinyl-CoA
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complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
(R)-2-benzylsuccinyl-CoA
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
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recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
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alpha4, 4 * 45000, SDS-PAGE
200000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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alpha4, 4 * 45000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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Show AA Sequence (460 entries)
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