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Information on EC 1.3.8.3 - (R)-benzylsuccinyl-CoA dehydrogenase
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1.3.8.3 (R)-benzylsuccinyl-CoA dehydrogenaseIUBMB Comments
Requires FAD as prosthetic group. Unlike other acyl-CoA dehydrogenases, this enzyme exhibits high substrate- and enantiomer specificity; it is highly specific for (R)-benzylsuccinyl-CoA and is inhibited by (S)-benzylsuccinyl-CoA. Forms the third step in the anaerobic toluene metabolic pathway in Thauera aromatica. Ferricenium ion is an effective artificial electron acceptor.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
(r)-benzylsuccinyl-coa dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
bbgS
BbsG
BbsG
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase
Requires FAD as prosthetic group. Unlike other acyl-CoA dehydrogenases, this enzyme exhibits high substrate- and enantiomer specificity; it is highly specific for (R)-benzylsuccinyl-CoA and is inhibited by (S)-benzylsuccinyl-CoA. Forms the third step in the anaerobic toluene metabolic pathway in Thauera aromatica. Ferricenium ion is an effective artificial electron acceptor.
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CAS REGISTRY NUMBER
COMMENTARY
260966-98-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate
(E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
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low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
Geobacter metallireducens ATCC 53774
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-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
Geobacter metallireducens DSM 7210
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
highly specific for (R)-benzylsuccinyl-CoA
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
no activity with benzylsuccinate or succinyl-CoA
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
involved in anaerobic toluene oxidation to benzoyl-CoA
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
Geobacter metallireducens ATCC 53774
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
Geobacter metallireducens DSM 7210
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
involved in anaerobic toluene oxidation to benzoyl-CoA
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
electron transferring flavoprotein
ETF, structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, binding kinetics analysis, overview. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins
electron transferring flavoprotein
structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: K0NC26 (etfA1) and K0NCE4 (etfB1). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview
electron transferring flavoprotein
structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q39VG4 (etfA) and Q39VG5 (etfB), binding kinetics analysis, overview. ETF crystal structure determination and analysis. Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins
electron transferring flavoprotein
structural and functional characterization of the electron transfer flavoprotein involved in toluene degradation in the strictly anaerobic bacteria, DNA and amino acid sequence determination and analysis, UniProt IDs: Q5NYM0 (etfA) and Q5NYL9 (etfB). Unrooted phylogenetic tree representing evolutionary branches of the ETFAB family proteins, overview
additional information
in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation
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additional information
in contrast to ETF, reduction of BbsG with dithionite leads directly to the fully reduced form without transient semiquinone formation
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.14
(R)-2-benzylsuccinyl-CoA
forward reaction, recombinant enzyme, pH 6.4, 50°C
0.098
(R)-2-benzylsuccinyl-CoA
forward reaction, recombinant enzyme, pH 6.4, 50°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
deletion of the BbsG gene has little impact on degradation of para-cresol. Geobacillus metallireducens uses a methylhydroxylase-initiated pathway for p-cresol degradation
physiological function
Geobacter metallireducens DSM 7210
-
deletion of the BbsG gene has little impact on degradation of para-cresol. Geobacillus metallireducens uses a methylhydroxylase-initiated pathway for p-cresol degradation
-
Show AA Sequence and Transmembrane Helices (901 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene bbsG, recombinant overexpression of the enzyme in Escherichia coli
gene bbsG, recombinant overexpression of the enzyme in Escherichia coli
gene bbsG, recombinant overexpression of the enzyme in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Leuthner, B.; Heider, J.
Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of b oxidation of the intermediate benzylsuccinate
J. Bacteriol.
182
272-277
2000
Thauera aromatica
brenda
Leutwein, C.; Heider, J.
(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway
Arch. Microbiol.
178
517-524
2002
Azoarcus sp., Aromatoleum tolulyticum, Thauera aromatica, Aromatoleum tolulyticum B5
brenda
Chaurasia, A.K.; Tremblay, P.L.; Holmes, D.E.; Zhang, T.
Genetic evidence that the degradation of para-cresol by Geobacter metallireducens is catalyzed by the periplasmic para-cresol methylhydroxylase
FEMS Microbiol. Lett.
362
pii: fnv145
2015
Geobacter metallireducens (Q39VG7), Geobacter metallireducens DSM 7210 (Q39VG7)
brenda
Vogt, M.; Schuehle, K.; Koelzer, S.; Peschke, P.; Chowdhury, N.; Kleinsorge, D.; Buckel, W.; Essen, L.; Heider, J.
Structural and functional characterization of an electron transfer flavoprotein involved in toluene degradation in strictly anaerobic bacteria
J. Bacteriol.
201
e00326-19
2021
Desulfobacula toluolica (K0NF66), Geobacter metallireducens (Q39VG7), Aromatoleum aromaticum (Q5P690), Thauera aromatica (Q9KJE8), Geobacter metallireducens GS-15 (Q39VG7), Geobacter metallireducens DSM 7210 (Q39VG7), Geobacter metallireducens ATCC 53774 (Q39VG7), Desulfobacula toluolica DSM 7467 (K0NF66), Aromatoleum aromaticum EbN1 (Q5P690), Desulfobacula toluolica Tol2 (K0NF66)
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