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benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
2-amino-benzoyl-CoA + reduced methyl viologen + ATP
2-amino-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 11% of the velocity of benzoyl-CoA
-
-
?
2-aminobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 18% of the activity relative to benzoyl-CoA
-
-
?
2-chloro-benzoyl-CoA + reduced methyl viologen + ATP
2-chloro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 106% of the velocity of benzoyl-CoA
-
-
?
2-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
2-fluoro-benzoyl-CoA + reduced methyl viologen + ATP
2-fluoro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 145% of the velocity of benzoyl-CoA
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
2-hydroxy-benzoyl-CoA + reduced methyl viologen + ATP
2-hydroxy-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 64% of the velocity of benzoyl-CoA
-
-
?
2-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
best substrate of enzyme BCRTar
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-fluoro-benzoyl-CoA + reduced methyl viologen + ATP
3-fluoro-cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
at 31% of the velocity of benzoyl-CoA
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 23% of the activity relative to benzoyl-CoA
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 12% of the activity relative to benzoyl-CoA
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-methylbenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 12% of the activity relative to benzoyl-CoA
-
-
?
4-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate
-
at 5% of the activity relative to benzoyl-CoA
-
-
?
azide + ATP + H2O
?
-
-
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
-
key enzyme of anaerobic aromatic metabolism
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + ferredoxin + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced ferredoxin + ATP + H2O
cyclohexa-1,5-diene-1-carboxyl-CoA + oxidized ferredoxin + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced methyl viologen + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + methyl viologen + ADP + phosphate
-
-
-
-
?
furan-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 28% of the velocity of benzoyl-CoA
-
-
?
N-methylhydroxylamine + ATP + H2O
?
-
-
-
-
?
NH2OH + ATP + H2O
?
-
-
-
-
?
O-methylhydroxylamine + ATP + H2O
?
-
-
-
-
?
pyridine-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 67% of the velocity of benzoyl-CoA
-
-
?
pyridine-4-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 41% of the velocity of benzoyl-CoA
-
-
?
thiophene-2-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 144% of the velocity of benzoyl-CoA
-
-
?
thiophene-3-carbonyl-CoA + reduced methyl viologen + ATP
? + methyl viologen + ADP + phosphate
-
at 10% of the velocity of benzoyl-CoA
-
-
?
additional information
?
-
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 78% of the activity relative to benzoyl-CoA
-
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
?
-
at 10% of the activity relative to benzoyl-CoA
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
ir
benzoyl-CoA + reduced acceptor + ATP
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
acceptor: Ti3+, reduced methyl viologen
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
-
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
the enzyme is involved in the anaerobic catabolism of benzoate
-
-
?
additional information
?
-
-
in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity
-
-
?
additional information
?
-
-
no substrate: furan-3-carbonyl-CoA, pyrrole-2-carbonyl-CoA, pyridine-4-carbonyl-CoA, 4-fluoro-benzoyl-CoA
-
-
?
additional information
?
-
-
reduction of aromatic ring requires 2 mol of ATP per mol of benzoyl-CoA
-
-
?
additional information
?
-
enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. Neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Enzyme BCRTar also shows no or poor activity with 4-methylbenzoyl-CoA, 4-ethylbenzoyl-CoA, 4-chlorobenzoyl-CoA, 2-bromobenzoyl-CoA, 4-bromobenzoyl-CoA, and 2-hydroxybenzoyl-CoA. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
-
-
-
additional information
?
-
-
enzyme MBRTcl preferentially dearomatizes meta-substituted BzCoA analogues containing methyl-, chloro-, or hydroxy-functionalities. With these substrates, relative specific activities compared with BzCoA are substantially higher with enzyme MBRTcl than with BCRTar from Thauera aromatica. MBRTcl also converts para-substituted halo- and methyl-BzCoA analogues that are not converted by BCRTar. Exceptions are 3-fluoro- and 4-fluorobenzoyl-CoA that serve as substrates for both enzymes. Neither of the two enzymes reduces heterocyclic nicotinoyl-CoA, and 4-hydroxybenzoyl-CoA is a poor substrate for both enzymes. Enzyme BCRTar also shows no or poor activity with 4-methylbenzoyl-CoA, 4-ethylbenzoyl-CoA, 4-chlorobenzoyl-CoA, 2-bromobenzoyl-CoA, 4-bromobenzoyl-CoA, and 2-hydroxybenzoyl-CoA. Formation of the corresponding two electron-reduced 1,5-dienoyl-CoA analogues is confirmed by ESI-Q-TOF-MS analysis, substrate specificity, overview. The activity enzyme assay uses Ti(III) citrate as artificial electron donor
-
-
-
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benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
central enzyme in the anaerobic degradation of organic carbon
-
-
?
2-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
2-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
2-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
2-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
best substrate of enzyme BCRTar
-
-
?
3-chlorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-chlorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
3-hydroxybenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
3-methylbenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
3-methylcyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
-
-
?
4-fluorobenzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
4-fluorocyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
low activity
-
-
?
Benzoyl-CoA + reduced acceptor + ATP
?
-
key enzyme of anaerobic aromatic metabolism
-
-
?
benzoyl-CoA + reduced acceptor + ATP + H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
the enzyme is involved in the anaerobic catabolism of benzoate
-
-
?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
high activity
-
-
?
benzoyl-CoA + reduced ferredoxin + ATP + H2O
cyclohexa-1,5-diene-1-carboxyl-CoA + oxidized ferredoxin + ADP + phosphate
-
-
-
-
?
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Schneider, S.; Mohamed, M.E.S.; Fuchs, G.
Anaerobic metabolism of L-phenylalanine via benzoyl-CoA in the denitrifying bacterium Thauera aromatica
Arch. Microbiol.
168
310-320
1997
Thauera aromatica
brenda
Boll , M.; Fuchs, G.
Identification, and characterization of the natural electron donor ferredoxin and of FAD as a possible prosthetic group of benzoyl-CoA reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism
Eur. J. Biochem.
251
946-954
1998
Thauera aromatica
brenda
Boll, M.; Albracht, S.S.P.; Fuchs, G.
Benzoyl-CoA reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. A study of adenosinetriphosphatase activity, ATP stoichiometry of the reaction and EPR properties of the enzyme
Eur. J. Biochem.
244
840-851
1997
Thauera aromatica, Thauera aromatica K172
brenda
Boll, M.; Fuchs, G.
Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thaurea aromatica strain K172
Eur. J. Biochem.
234
921-933
1995
Thauera aromatica
brenda
Breese, K.; Boll, M.; Alt-Morbe, J.; Schagger, H.; Fuchs, G.
Genes coding the benzoyl-CoA pathway of anaerobic metabolism in the bacterium Thauera aromatica
Eur. J. Biochem.
256
148-154
1998
Thauera aromatica
brenda
Boll, M.; Laempe, D.; Eisenreich, W.; Bachers, A.; Mittelberger, T.; Heinze, J.; Fuchs, G.
Nonaromatic products from anoxic conversion of benzoyl-CoA with benzoyl-CoA reductase and cyclohexa-1,5-diene-1-carbonyl-CoA hydratase
J. Biol. Chem.
275
21889-21895
2000
Thauera aromatica
brenda
Heider, J.; Boll, M.; Breese, K.; Breinig, S.; Ebenau-Jehle, C.; Feil, U.; Gad'on, N.; Laempe, D.; Leuthner, B.; Mohamed, M.E.S.; Schneider, S.; Burchhardt, G.; Fuchs, G.
Differential induction of enzymes involved in anaerobic metabolism of aromatic compounds in the denitrifying bacterium Thauera aromatica
Arch. Microbiol.
170
120-131
1998
Thauera aromatica
brenda
Mobitz, H.; Friedrich, T.; Boll, M.
Substrate binding and reduction of benzoyl-CoA reductase: evidence for nucleotide-dependent conformational changes
Biochemistry
43
1376-1385
2004
Thauera aromatica
brenda
Unciuleac, M.; Boll, M.
Mechanism of ATP-driven electron transfer catalyzed by the benzene ring-reducing enzyme benzoyl-CoA reductase
Proc. Natl. Acad. Sci. USA
98
13619-13624
2001
Thauera aromatica
brenda
Song, B.; Ward, B.B.
Genetic diversity of benzoyl coenzyme a reductase genes detected in denitrifying isolates and estuarine sediment communities
Appl. Environ. Microbiol.
71
2036-2045
2005
Acidovorax sp., Acidovorax sp. 2FB7, Aromatoleum evansii (Q8VUG0), Aromatoleum evansii (Q8VUG1), Aromatoleum evansii (Q8VUG2), Aromatoleum evansii (Q8VUG3), Aromatoleum evansii, Aromatoleum toluclasticum, Aromatoleum tolulyticum (Q4Z8X5), Aromatoleum toluvorans, Magnetospirillum magnetotacticum, Rhodopseudomonas palustris (O07460), Rhodopseudomonas palustris (O07461), Rhodopseudomonas palustris (O07462), Rhodopseudomonas palustris (O07463), Rhodopseudomonas palustris, Thauera aromatica (O87876), Thauera aromatica, Thauera chlorobenzoica, Thauera chlorobenzoica 3CB-1, Thauera selenatis, Thauera selenatis 3CB-1
brenda
Carmona, M.; Diaz, E.
Iron-reducing bacteria unravel novel strategies for the anaerobic catabolism of aromatic compounds
Mol. Microbiol.
58
1210-1215
2005
Azoarcus sp., Thauera aromatica
brenda
Thiele, B.; Rieder, O.; Golding, B.T.; Mueller, M.; Boll, M.
Mechanism of enzymatic Birch reduction: stereochemical course and exchange reactions of benzoyl-CoA reductase
J. Am. Chem. Soc.
130
14050-14051
2008
Thauera aromatica
brenda
Tiedt, O.; Fuchs, J.; Eisenreich, W.; Boll, M.
A catalytically versatile benzoyl-CoA reductase, key enzyme in the degradation of methyl- and halobenzoates in denitrifying bacteria
J. Biol. Chem.
293
10264-10274
2018
Thauera aromatica (O87876 AND O87875 AND O87874 AND O87877), Thauera aromatica, Thauera chlorobenzoica (A0A1H5S3R7 AND A0A1L6FDJ2 AND A0A1H5S371 AND A0A1L6FDS4), Thauera chlorobenzoica
brenda