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Information on EC 1.3.7.7 - ferredoxin:protochlorophyllide reductase (ATP-dependent) and Organism(s) Auxenochlorella protothecoides and UniProt Accession Q6VQA9

for references in articles please use BRENDA:EC1.3.7.7
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EC Tree
IUBMB Comments
Occurs in photosynthetic bacteria, cyanobacteria, green algae and gymnosperms. The enzyme catalyses trans-reduction of the D-ring of protochlorophyllide; the product has the (7S,8S)-configuration. Unlike EC 1.3.1.33 (protochlorophyllide reductase), light is not required. The enzyme contains a [4Fe-4S] cluster, and structurally resembles the Fe protein/MoFe protein complex of nitrogenase (EC 1.18.6.1), which catalyses an ATP-driven reduction.
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Auxenochlorella protothecoides
UNIPROT: Q6VQA9 not found.
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The taxonomic range for the selected organisms is: Auxenochlorella protothecoides
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
light-independent protochlorophyllide reductase, dark protochlorophyllide reductase, light-independent pchlide reductase, dark-operative pchlide oxidoreductase, dark-operative protochlorophyllide reductase, light-independent (dark-operative) pchlide oxidoreductase, protochlorophyllide oxidoreductase complex, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
light-independent protochlorophyllide oxidoreductase
-
light-independent protochlorophyllide reductase
-
SYSTEMATIC NAME
IUBMB Comments
ATP-dependent ferredoxin:protochlorophyllide-a 7,8-oxidoreductase
Occurs in photosynthetic bacteria, cyanobacteria, green algae and gymnosperms. The enzyme catalyses trans-reduction of the D-ring of protochlorophyllide; the product has the (7S,8S)-configuration. Unlike EC 1.3.1.33 (protochlorophyllide reductase), light is not required. The enzyme contains a [4Fe-4S] cluster, and structurally resembles the Fe protein/MoFe protein complex of nitrogenase (EC 1.18.6.1), which catalyses an ATP-driven reduction.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chlorophyllide a + reduced ferredoxin + ATP
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chlorophyllide a + reduced ferredoxin + ATP
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4Fe-4S-center
the enzyme contains an Fe-S cluster as redox center in all subunits
ATP
subunit L contains the ATP-binding motif
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunit B, subunit L, and subunit N; strain CS-41, also named Auxenochlorella protothecoides strain CS-41
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
light-independent protochlorophyllide reductase is required for protochlorophyllide reduction in the dark
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHLN_AUXPR
435
0
49391
Swiss-Prot
other Location (Reliability: 2)
CHLL_AUXPR
300
0
32843
Swiss-Prot
other Location (Reliability: 5)
CHLB_AUXPR
510
0
57539
Swiss-Prot
Mitochondrion (Reliability: 2)
A0A023HHY6_AUXPR
288
0
31620
TrEMBL
other Location (Reliability: 5)
A0A023HHU5_AUXPR
510
0
57341
TrEMBL
Mitochondrion (Reliability: 3)
A0A023HHS7_AUXPR
437
0
49508
TrEMBL
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE
49000
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE
58000
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)Lys cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the level of theLIPOR subunit L mRNA of Chlorella protothecoides strain CS-41 is much higher in the light than in the dark
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shi, C.; Shi, X.
Characterization of three genes encoding the subunits of light-independent protochlorophyllide reductase in Chlorella protothecoides CS-41
Biotechnol. Prog.
22
1050-1055
2006
Auxenochlorella protothecoides (Q6VQA8 and Q6VQA9 and Q7YKW4), Auxenochlorella protothecoides
Manually annotated by BRENDA team