Substrates: the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis Products: -
Substrates: the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis Products: -
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
the two genes PPRD1 and PPRD2 are orthologous to human SRD5A3 (steroid 5alpha reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana
the two genes PPRD1 and PPRD2 are orthologous to human SRD5A3 (steroid 5alpha reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana
congenital disorder of glycosylation (CDG) caused by SRD5A3 deficiency, SRD5A3-CDG is the disorder results from deleterious mutations in SRD5A3, phenotype analysis with emphasis on adult onset features and variable eye and skin involvement, including including kyphosis, retinitis pigmentosa, and cataracts, detailed overview
polyprenol reductase2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. PPRD2 deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes might also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. Supplementation of the human diet with dolichol-enriched plant tissues can allow therapeutic interventions in glycosylation disorders
polyprenol reductase2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. PPRD2 deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes might also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. Supplementation of the human diet with dolichol-enriched plant tissues can allow therapeutic interventions in glycosylation disorders
the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis
isozymes PPRD1 and PPRD2 are the factors mediating the key step of the dolichol cycle in plant cells. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression
isozymes PPRD1 and PPRD2 are the factors mediating the key step of the dolichol cycle in plant cells. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of isozyme PPRD1 in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of wild-type an dmutant isozyme PPRD1s in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis