Information on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
1.3.1.84
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RECOMMENDED NAME
GeneOntology No.
acrylyl-CoA reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
propanoyl-CoA + NADP+ = acryloyl-CoA + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-hydroxypropanoate cycle
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3-hydroxypropanoate/4-hydroxybutanate cycle
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glyoxylate assimilation
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CO2 fixation in Crenarchaeota
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Carbon fixation pathways in prokaryotes
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:NADP+ oxidoreductase
Catalyses a step in the 3-hydroxypropanoate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [1]. The enzyme from Sulfolobus tokodaii does not act on either NADH or crotonyl-CoA [2]. Different from EC 1.3.1.8, which acts only on enoyl-CoA derivatives of carbon chain length 4 to 16. Contains Zn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
150386-12-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acryloyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
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-
-
?
acrylyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
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-
-
-
?
acrylyl-CoA + NADPH + H+
propionyl-CoA + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acrylyl-CoA + NADPH + H+
propionyl-CoA + NADP+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
acryloyl-CoA
pH 6.0, 65C
0.0011 - 0.0028
acrylyl-CoA
0.018 - 0.036
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45 - 80
acrylyl-CoA
13
NADPH
pH 6.0, 65C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18.7
pH 6.0, 65C
23
cell extract, at pH 7.0 and 30C
72
-
after 6fold purification, at pH 7.0 and 30C
98
after 4fold purification, at pH 7.0 and 30C
130
after 11fold purification, at pH 7.0 and 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
half-maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38600
1 * 39000, SDS-PAGE, 1 * 38600, calculated
39000
1 * 39000, SDS-PAGE, 1 * 38600, calculated
40000
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x * 40000, SDS-PAGE
43000
gel filtration
56000
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gel filtration
62000
gel filtration
64000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 40000, SDS-PAGE
homodimer
monomer
1 * 39000, SDS-PAGE, 1 * 38600, calculated
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 30% glycerol, stable for months
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superose 12 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta2 (DE3) cells
Show AA Sequence (260 entries)
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