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Information on EC 1.3.1.72 - DELTA24-sterol reductase and Organism(s) Mus musculus and UniProt Accession Q8VCH6
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1.3.1.72 DELTA24-sterol reductaseIUBMB Comments
Acts on a range of steroids with a 24(25)-double bond, including lanosterol, desmosterol and zymosterol.
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Word Map
- 1.3.1.72
- alzheimer
-
24-dehydrocholesterol
-
indicator-1
-
desmosterolosis
- ebp
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smith-lemli-opitz
- triparanol
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post-squalene
- medicine
- agriculture
- pharmacology
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
dhcr24, seladin-1, dwarf1, 24-dehydrocholesterol reductase, 24-reductase, dehydrocholesterol reductase, diminuto/dwarf1, delta24-reductase, zmdwf1, desmosterol reductase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
24,25-reductase
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24,25-sterol reductase
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24-reductase
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C-24 reductase
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DELTA24 reductase
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DELTA24-reductase
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desmosterol reductase
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hydroxy steroid DELTA24-reductase
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lanosterol 24-reductase
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lanosterol DELTA24-reductase
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lanosterol reductase
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reductase, hydroxy steroid DELTA24-
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sterol 24,25-reductase
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sterol 24-reductase
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sterol DELTA24 reductase
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sterol DELTA24-reductase
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sterol-DELTA24-reductase
sterol-DELTA24-reductase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
BRENDA
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-, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
sterol:NADP+ DELTA24-oxidoreductase
Acts on a range of steroids with a 24(25)-double bond, including lanosterol, desmosterol and zymosterol.
CAS REGISTRY NUMBER
COMMENTARY
9033-57-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5alpha-cholest-7-en-3beta-ol + NADP+
5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
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r
5alpha-cholesta-7,24-dien-3beta-ol + NADPH
5alpha-cholest-7-en-3beta-ol + NADP+
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?
additional information
?
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intracellular generation of reactive oxygen species in response to H2O2 is diminished in the presence of DHCR24, suggesting a reactive oxygen species-scavenging activity of DHCR24
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5alpha-cholest-7-en-3beta-ol + NADP+
5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
abundantly expressed throughout the epidermis except for the stratum corneum and in hair follicles
additional information
additional information
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no activity in J774 cells, that lack a full-length transcript for DHCR24
additional information
-
no activity in J774 cells, that lack a full-length transcript for DHCR24. J774 cells are able to grow indefinitely after the virtually total replacement of cholesterol by desmosterol, i.e. J774-D cells
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
additional information
malfunction
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DHCR24 knockout mice die within a few hours after birth. Cultured metatarsal bones from newborn knockout mice show a significant retarded growth. Absence of proliferating chondrocytes in the growth plate and abnormal hypertrophy of prehypertrophic chondrocytes are observed in the bones from knockout mice
malfunction
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insulin-induced reactive oxygen species production is enhanced by siRNA for DHCR24
malfunction
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overexpression of DHCR24 protects ATDC5 cells from H2O2-induces hypertrophy. Insulin-induced hypertrophic differentiation of ATDC5 cells is associated with production of reactive oxygen species and pretreatment of the cells with siRNA for DHCR24 mRNA accelerates the hypertrophic differentiation
malfunction
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enzyme inhibition leads to increased inflammation resolution and selectively decreases proinflammatory cell influx
metabolism
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desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, and desmosterol can
metabolism
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desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, while 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, can do so, pathway regulation, detailed overview
additional information
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J774 cells, that lack a full-length transcript for DHCR24, consequently accumulate desmosterol
additional information
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J774 cells, that lack a full-length transcript for DHCR24, consequently accumulate desmosterol
additional information
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while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required
additional information
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while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DHC24_MOUSE
516
2
60112
Swiss-Prot
Secretory Pathway (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
DHCR24 knockout mice, development of the epidermis, especially keratinization, is impaired, altered expression of filaggrin, loricrin, and involcrin, transepidermal water loss is markedly increased, impaired skin barrier function
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Mus musculus embryonic fibroblasts and PC-12 cells infected with adenovirus
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activation of constitutive androstane receptor (CAR) in mouse livers increases mRNA levels of mouse Dhcr24 and human DHCR24
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
DHCR24 plays crucial role for skin development and its proper function
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mirza, R.; Hayasaka, S.; Takagishi, Y.; Kambe, F.; Ohmori, S.; Maki, K.; Yamamoto, M.; Murakami, K.; Kaji, T.; Zadworny, D.; Murata, Y.; Seo, H.
DHCR24 gene knockout mice demonstrate lethal dermopathy with differentiation and maturation defects in the epidermis
J. Invest. Dermatol.
126
638-647
2006
Mus musculus (Q8VCH6)
Lu, X.; Kambe, F.; Cao, X.; Kozaki, Y.; Kaji, T.; Ishii, T.; Seo, H.
3beta-Hydroxysteroid-DELTA24 reductase is a hydrogen peroxide scavenger, protecting cells from oxidative stress-induced apoptosis
Endocrinology
149
3267-3273
2008
Mus musculus
Rodriguez-Acebes, S.; de la Cueva, P.; Fernandez-Hernando, C.; Ferruelo, A.J.; Lasuncion, M.A.; Rawson, R.B.; Martinez-Botas, J.; Gomez-Coronado, D.
Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-DELTA24-reductase-deficient cell line
Biochem. J.
420
305-315
2009
Mus musculus
Stevenson, J.; Brown, A.J.
How essential is cholesterol?
Biochem. J.
420
e1-e4
2009
Mus musculus
Mirza, R.; Qiao, S.; Tateyama, K.; Miyamoto, T.; Xiuli, L.; Seo, H.
3beta-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species
J. Bone Miner. Metab.
30
144-153
2012
Mus musculus
Yoshinari, K.; Ohno, H.; Benoki, S.; Yamazoe, Y.
Constitutive androstane receptor transactivates the hepatic expression of mouse Dhcr24 and human DHCR24 encoding a cholesterogenic enzyme 24-dehydrocholesterol reductase
Toxicol. Lett.
208
185-191
2012
Homo sapiens, Mus musculus
Koerner, A.; Zhou, E.; Mueller, C.; Mohammed, Y.; Herceg, S.; Bracher, F.; Rensen, P.C.N.; Wang, Y.; Mirakaj, V.; Giera, M.
Inhibition of DELTA24-dehydrocholesterol reductase activates pro-resolving lipid mediator biosynthesis and inflammation resolution
Proc. Natl. Acad. Sci. USA
116
20623-20634
2019
Mus musculus
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