Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.3.1.70 - DELTA14-sterol reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LDR4

for references in articles please use BRENDA:EC1.3.1.70
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This enzyme acts on a range of steroids with a 14(15)-double bond.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LDR4
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
lamin b receptor, erg24, tm7sf2, 14-reductase, sterol c-14 reductase, dhcr14, c14sr, fackel, delta14-reductase, fk-j3158, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14-reductase
-
-
-
-
C-14 reductase
-
-
-
-
C-14 sterol reductase
-
-
-
-
DELTA14-reductase
-
-
-
-
DELTA14-SR
-
-
-
-
DELTA14SR
-
-
-
-
DELTA7,14-steroid 14-reductase
-
-
-
-
DELTA7,14-sterol 14-reductase
-
-
-
-
DELTA7,14-sterol DELTA14-reductase
-
-
-
-
DELTA7,14-sterol-14-reductase
-
-
-
-
DELTA8,14-hydroxy steroid DELTA14(15)-reductase
-
-
-
-
DELTA8,14-steroid 14-reductase
-
-
-
-
DELTA8,14-sterol 14-reductase
-
-
-
-
DELTA8,14-sterol DELTA14-reductase
-
-
-
-
DELTA8,14-sterol-14-reductase
-
-
-
-
FACKEL protein
-
-
-
-
reductase, DELTA8,14-hydroxy steroid DELTA14(15)-
-
-
-
-
sterol 14-reductase
-
-
-
-
sterol C-14 reductase
sterol C14-reductase
-
-
-
-
sterol DELTA14,15-reductase
-
-
-
-
sterol DELTA14-reductase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol:NADP+ DELTA14-oxidoreductase
This enzyme acts on a range of steroids with a 14(15)-double bond.
CAS REGISTRY NUMBER
COMMENTARY hide
69403-07-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
4alpha-methylfecosterol + NADP+
show the reaction diagram
isofucosterol + NADPH
sitosterol + NADP+
show the reaction diagram
additional information
?
-
-
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+
4alpha-methylfecosterol + NADP+
show the reaction diagram
-
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by enzyme FK
-
-
?
isofucosterol + NADPH
sitosterol + NADP+
show the reaction diagram
-
step in the biosynthesis of the brassicasterol phytohormones, catalyzed by bifunctinal enzyme DIM1/CBB1/DWF1
-
-
?
additional information
?
-
-
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
fenpropimorph
-
impairs cell expansion and affects gene expression in vivo, the effects cannot be reversed by addition of exogenous brassicasterols, changes sterol composition, phenotype is similar to fk-mutant plants, molecular mechanism
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
brassicasterols have regulatory function in the plants concerning growth and development, the enzyme is up-regulated by several growth-promoting hormones including brassinolide and auxin, overview
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
fk gene expression pattern in wild-type and mutant strains, regulatory effects of sterols, overview
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
Arabidopsis thaliana weak mutant allele fk-J3158, impaired in the FACKEL (FK) gene, which encodes the C-14 reductase, has a long life cycle and delayed flowering time in different photoperiods. Enzyme overexpression lines display an earlier flowering phenotype than that of the wild-type, flowering time of fk-J3158 flc double mutant is significantly earlier than that of fk-J3158 under the long-day conditions. These processes might be independent of the downstream brassinosteroid pathway and the autonomous pathway. The fk-J3158 plants are more sensitive than wild-type in reducing the bolting days and total leaf number under gibberellic acid treatment. Enzyme mutation leads to an absence of endogenous gibberellic acids in fk-J3158 and FK gene expression also affected under gibberellic acid and paclobutrazol treatment. The delayed flowering time of fk-J3158 is rescued by a 3-week vernalization treatment, and the expression of flowering locus C C (FLC) is accordingly downregulated in fk-J3158. Flowering time of fk-J3158 flc double mutant is significantly earlier than that of fk-J3158 under the long-day conditions. Phenotypes, detailed overview
physiological function
the enzyme is involved in sterol biosynthesis, but is also involved in the flowering of Arabidopsis mainly via the gibberellin pathway and vernalization pathway. This function of the enzyme is partially dependent on the FLOWERING LOCUS C (FLC)
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ERG24_ARATH
369
9
41913
Swiss-Prot
Secretory Pathway (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
mutational blockage of the enzyme leads to accumulation of atypical products from substrate 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol in vivo, i.e. 5alpha-cholesta-8,14-dien-3beta-ol, (24R)-5alpha-ergosta-8,14-dien-3beta-ol, and (24R)-5alpha-stigmasta-8,14-dien-3beta-ol
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
He, J.X.; Fujioka, S.; Li, T.C.; Kang, S.G.; Seto, H.; Takatsuto, S.; Yoshida, S.; Jang, J.C.
Sterols regulate development and gene expression in Arabidopsis
Plant Physiol.
131
1258-1269
2003
Arabidopsis thaliana
Manually annotated by BRENDA team
Huang, B.; Qian, P.; Gao, N.; Shen, J.; Hou, S.
Fackel interacts with gibberellic acid signaling and vernalization to mediate flowering in Arabidopsis
Planta
245
939-950
2017
Arabidopsis thaliana (Q9LDR4)
Manually annotated by BRENDA team