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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
zeatin reductase,
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EC 1.1.1.242
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formerly
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reductase, zeatin
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dihydrozeatin + NADP+ = zeatin + NADPH + H+
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dihydrozeatin:NADP+ oxidoreductase
Previously classified erroneously as EC 1.1.1.242.
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trans-zeatin + NADPH
dihydrozeatin + NADP+
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zeatin + NADPH
dihydrozeatin + NADP+
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zeatin + NADPH + H+
dihydrozeatin + NADP+
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additional information
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no activity with: ribosylzeatin, cis-zeatin, O-xylosylzeatin, N6-(DELTA2-isopentenyl)adenine, or N6-(DELTA2-isotentenyl)adenosine
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trans-zeatin + NADPH
dihydrozeatin + NADP+
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NADPH
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NADPH
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required, NADH cannot substitute for NADPH
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diethyldithiocarbamic acid
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10 mM, completely suppressed in vitro
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0.07
trans-zeatin
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high molecular weight isoenzyme
0.1 - 0.23
trans-zeatin
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low molecular weight isoenzyme, instability of the isoenzyme after gel filtration may contibute to the variation between experiments
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additional information
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brenda
L. cv. Gotik and L. cv. Canis
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brenda
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immature
brenda
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brenda
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brenda
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25000
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low molecular weight isoenzyme, gel filtration
55000
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high molecular weight isoenzyme, gel filtration
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glycerol, 20%, stabilizes
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-20°C, in 0.05 M phosphate buffer containing 5 mM DTT, 0.5 mM EDTA, 5 mM NADPH, 0.5 M KCl and 20% glycerol: loss of about 10% of activity after 3 days
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Martin, R.C.; Mok, M.C.; Shaw, G.; Mok, D.W.
An enzyme mediating the conversion of zeatin to dihydrozeatin in Phaseolus embryos
Plant Physiol.
90
1630-1635
1989
Phaseolus vulgaris
brenda
Gaudinova, A.; Dobrev, P.I.; Solcova, B.; Novak, O.; Strnad, M.; Friedecky, D.; Motyka, V.
The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
J. Plant Growth Regul.
24
188-200
2005
Pisum sativum
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brenda
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1.3.1.69 zeatin reductase
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