Information on EC 1.3.1.57 - phloroglucinol reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.57
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RECOMMENDED NAME
GeneOntology No.
phloroglucinol reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrophloroglucinol + NADP+ = phloroglucinol + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gallate degradation III (anaerobic)
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Aminobenzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
dihydrophloroglucinol:NADP+ oxidoreductase
Involved in the gallate anaerobic degradation pathway in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
80804-59-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phloroglucinol + NADPH
dihydrophloroglucinol + NADP
show the reaction diagram
phloroglucinol + NADPH
dihydrophloroglucinol + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phloroglucinol + NADPH
dihydrophloroglucinol + NADP
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-Trihydroxybenzoate
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tested at 1.0 mM and under N2, 49% inhibition
6-Hydroxynicotinate
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tested at 1.0 mM, 12% inhibition
8-hydroxyquinoline
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 83% inhibition
catechol
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tested at 1.0 mM, 14% inhibition
CuCl2
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tested at 0.1 mM, 100% inhibition
diethyldicarbonate
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tested at 1.0 mM, 54% inhibition
EDTA
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enzyme preincubated with the inhibitor, 0.00333 mM, for 60 min at room temperature before the addition of the substrate, 11% inhibition
gallate
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tested at 1.0 mM and under N2, 24% inhibition
hydroquinone
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tested at 1.0 mM and under N2, 31% inhibition
hydroxylamine
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 9% inhibition
iodacetate
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 21% inhibition
menadione
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tested at 1.0 mM, 33% inhibition
N-ethylmaleimide
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tested at 1.0 mM, 94% inhibition
NAD+
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with 0.33 mM, 15% inhibition
NADH
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with 0.33 mM, 11% inhibition
NADP+
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with 0.33 mM, 38% inhibition
o-phenanthroline
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 71% inhibition
p-chloromercuribenzoate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
dihydrophloroglucinol
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reverse direction at pH 7.2
0.027
NADP+
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reverse direction at pH 7.2
0.00003 - 0.0067
NADPH
0.00003 - 0.8
phloroglucinol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.08
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crotonate-grown cells
4.18
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gallate-formate-grown cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
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2 * alpha, 2 * 33000, gel electrophoresis
78000
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gel filtration chromatography with native enzyme
130000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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crude enzyme shows a loss activity over a 5-day period, in presence of 1 mM of either 2-mercaptoethanol, dithiothreitol, or glutathione, or combinations of these reagents, is protected from the loss of enzyme activity
additional information
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a rapid decay of the enzyme activity in water bath at 53°C, less then 10% of the initial activity remains after 2.5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for several hours when kept on ice and retained full activity being frozen with 15% glycerol in liquid N2. Incubation at 30°C and pH 7.0, 82% of initial activity retained after 3.3 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
prepared of a cell extract by French press, DEAE-cellulose column, phenyl-Sepharose column, ultrafiltration, red agarose column, anion exchange column, purified 90.4fold with a yield of 29%
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protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration
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