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IUBMB Comments In the reverse reaction, the 2′-hydroxyisoflavone (2′-hydroxydaidzein) is reduced to an isoflavanone. Also acts on 2′-hydroxyformononetin and to a small extent on 2′-hydroxygenistein. Involved in the biosynthesis of the phytoalexin glyceollin. The isoflavones biochanin A, daidzein and genestein as well as the flavonoids apigenin, kaempferol and quercetin do not act as substrates.
Synonyms
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NADPH:2'-hydroxydaidzein oxidoreductase
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Reductase, 2'-hydroxydaidzein
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2'-hydroxy-2,3-dihydrodaidzein + NADP+ = 2'-hydroxydaidzein + NADPH + H+
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MetaCyc
(-)-glycinol biosynthesis, coumestrol biosynthesis, kievitone biosynthesis
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2'-hydroxy-2,3-dihydrodaidzein:NADP+ 2'-oxidoreductase
In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. Also acts on 2'-hydroxyformononetin and to a small extent on 2'-hydroxygenistein. Involved in the biosynthesis of the phytoalexin glyceollin. The isoflavones biochanin A, daidzein and genestein as well as the flavonoids apigenin, kaempferol and quercetin do not act as substrates.
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2'-Hydroxydaidzein + NADH
2'-Hydroxydihydrodaidzein + NAD+
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Substrates: 8% of the reaction with NADP+
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2'-Hydroxydaidzein + NADPH
2'-Hydroxydihydrodaidzein + NADP+
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Substrates: -
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2'-Hydroxyformomonetin + NADPH
2'-Hydroxydihydroformonetin + NADP+
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Substrates: -
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2'-Hydroxygenistein + NADPH
2'-Hydroxydihydrogenistein + NADP+
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Substrates: 10% of the reaction relative to 2'-hydroxydaidzein
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additional information
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additional information
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Substrates: no activity with: biochanin A, daidzein, genistein, apigenin, kaempferol, quercetin
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additional information
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Substrates: specific role in the biosynthesis of glyceollin
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additional information
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Substrates: specific role in the biosynthesis of glyceollin
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0.05
2'-Hydroxydaidzein
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0.06
2'-Hydroxyformomonetin
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additional information
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5.5 - 9
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pH 5.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity
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brenda
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elicitor-induced
brenda
Highest Expressing Human Cell Lines
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monomer
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1 * 34700, SDS-PAGE
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Fischer, D.; Ebenau-Jehle, C.; Grisebach, H.
Phytoalexin synthesis in soybean: purification and characterization of NADPH: 2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell culture
Arch. Biochem. Biophys.
276
390-395
1990
Glycine max
brenda
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