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Information on EC 1.3.1.31 - 2-enoate reductase and Organism(s) Kluyveromyces lactis and UniProt Accession P40952

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EC Tree
     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.31 2-enoate reductase
IUBMB Comments
An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl alphabeta-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
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This record set is specific for:
Kluyveromyces lactis
UNIPROT: P40952
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Word Map
The taxonomic range for the selected organisms is: Kluyveromyces lactis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
enoate reductase, yellow enzyme, lacer, 2-enoate reductase, nadph dehydrogenase 1, achr-oye4, kpner, er-ca, achr-oye3, sye-4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
enoate reductase
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NADPH dehydrogenase 1
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old yellow enzyme
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acylate:NAD+ DELTA2 oxidoreductase
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-
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enoate reductase
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-
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reductase, 2-enoate
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
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-
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reduction
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-
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dehydrogenation
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-
-
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SYSTEMATIC NAME
IUBMB Comments
butanoate:NAD+ DELTA2-oxidoreductase
An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl alphabeta-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
CAS REGISTRY NUMBER
COMMENTARY hide
70712-51-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-carvone + NADPH
2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
show the reaction diagram
-
-
-
?
2-cyclohexen-1-one + NADH
2-cyclohexanone + NAD+
show the reaction diagram
-
-
-
?
3-methyl-2-butenal + NADPH
3-methylbutanal + NADP+
show the reaction diagram
-
-
-
?
but-3-en-2-one + NADPH
butan-2-one + NADP+
show the reaction diagram
-
-
-
?
cinnamaldehyde + NADPH
?
show the reaction diagram
-
-
-
?
citral + NADPH
3-phenylpropanal + NADP+
show the reaction diagram
-
-
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADPH
? + NADP+
show the reaction diagram
-
-
-
?
ketoisophorone + NADPH
(6R)-levodione
show the reaction diagram
-
-
-
?
maleic anhydride + NADPH
dihydrofuran-2,5-dione + NADP+
show the reaction diagram
-
-
-
?
maleimide + NADH + H+
pyrrolidine-2,5-dione + NAD+
show the reaction diagram
-
-
-
?
N-ethylmaleimide + NADPH
N-ethylpyrrolidine-2,5-dione + NADP+
show the reaction diagram
-
-
-
?
trans,trans-2,4-hexadienal + NADPH
?
show the reaction diagram
-
-
-
?
additional information
?
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NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
the enzymes shows very little or no activity with NADH
NADPH
preferred cofactor
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.46
highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 8585D-5
UniProt
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 45
KYE1 has a half-life of 1146 min at 30°C, 210 min at 37°C, and less than 5 min at 45°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA bead chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chaparro-Riggers, J.F.; Rogers, T.A.; Vazquez-Figueroa, E.; Polizzi, K.M.; Bommarius, A.S.
Comparison of three enoate reductases and their potential use for biotransformations
Adv. Synth. Catal.
349
1521-1531
2007
Yersinia bercovieri, Kluyveromyces lactis (P40952), Pseudomonas putida (Q9R9V9)
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Manually annotated by BRENDA team