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Information on EC 1.3.1.22 - 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+) and Organism(s) Solanum lycopersicum and UniProt Accession Q5K2N1

for references in articles please use BRENDA:EC1.3.1.22
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EC Tree
IUBMB Comments
The enzyme catalyses the conversion of assorted 3-oxo-Delta4 steroids into their corresponding 5alpha form. Substrates for the mammalian enzyme include testosterone, progesterone, and corticosterone. Substrates for the plant enzyme are brassinosteroids such as campest-4-en-3-one and (22alpha)-hydroxy-campest-4-en-3-one. cf. EC 1.3.99.5, 3-oxo-5alpha-steroid 4-dehydrogenase (acceptor).
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This record set is specific for:
Solanum lycopersicum
UNIPROT: Q5K2N1
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Word Map
The taxonomic range for the selected organisms is: Solanum lycopersicum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
5alphar, 5 alpha-reductase type 2, 5alpha-reductase 1, 5alpha-r2, steroid 5-alpha reductase, steroid 5-alpha-reductase, 5-alpha-reductase type 2, 5 alpha-r2, 5-alpha-reductase type 1, steroid 5alpha-reductase type ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
steroid 5alpha-reductase
-
3-oxosteroid 5alpha-reductase
-
-
-
-
3-oxosteroid DELTA4-dehydrogenase
-
-
-
-
4-ene-3-oxosteroid 5alpha-reductase
-
-
-
-
4-ene-5alpha-reductase
-
-
-
-
cholest-4-en-3-one 5alpha-reductase
-
-
-
-
cortisone alpha-reductase
-
-
-
-
cortisone DELTA 4-5alphareductase
-
-
-
-
DELTA4-3-ketosteroid 5alpha-oxidoreductase
-
-
-
-
DELTA4-3-ketosteroid reductase (5alpha)
-
-
-
-
DELTA4-3-oxosteroid-5 alpha-reductase
-
-
-
-
DELTA4-5alpha-reductase
-
-
-
-
DELTA4-steroid 5alpha-reductase (progesterone)
-
-
-
-
microsomal steroid reductase (5alpha)
-
-
-
-
NADPH:DELTA4-3-oxosteroid-5alpha-oxidoreductase
-
-
-
-
progesterone 5alpha-reductase
-
-
-
-
reduced nicotinamide adenine dinucleotide phosphate:DELTA4-3-ketosteroid 5alpha-oxidoreductase
-
-
-
-
reductase, cholestenone 5alpha-
-
-
-
-
reductase, cortisone DELTA: 4-5alpha-
-
-
-
-
reductase, progesterone 5alpha-
-
-
-
-
steroid 5-alpha-reductase
-
-
-
-
steroid 5alpha-hydrogenase
-
-
-
-
steroid 5alpha-reductase
-
-
-
-
testosterone 5alpha-reductase
-
-
-
-
testosterone delta4-5alpha-reductase
-
-
-
-
testosterone delta4-hydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-oxo-5alpha-steroid:NADP+ DELTA4-oxidoreductase
The enzyme catalyses the conversion of assorted 3-oxo-Delta4 steroids into their corresponding 5alpha form. Substrates for the mammalian enzyme include testosterone, progesterone, and corticosterone. Substrates for the plant enzyme are brassinosteroids such as campest-4-en-3-one and (22alpha)-hydroxy-campest-4-en-3-one. cf. EC 1.3.99.5, 3-oxo-5alpha-steroid 4-dehydrogenase (acceptor).
CAS REGISTRY NUMBER
COMMENTARY hide
37255-34-8
-
72412-84-1
-
9029-09-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
androstenedione + NADPH + H+
5alpha-androstan-3,17-dione + NADP+
show the reaction diagram
-
-
-
?
campestenone + NADPH
5alpha-campestan-3-one + NADP+
show the reaction diagram
reduction at very low levels
-
-
?
progesterone + NADPH + H+
5alpha-pregnan-3-20-dione + NADP+
show the reaction diagram
-
-
-
?
testosterone + NADPH + H+
5alpha-dihydrotestosterone + NADP+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-MA
active at 0.01 mM
AFA131
active at 0.01 mM
AFA192
active at 0.01 mM
AFA27
active at 0.01 mM
AFA76
active at 0.01 mM, most potent inhibitor among non-steroid compounds tested
AS601811
active at 0.01 mM
finasteride
active at 0.01 mM
PD17
active at 0.01 mM
PD91
active at 0.01 mM
VG106
active at 0.01 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02634
androstenedione
-
0.00075
progesterone
-
0.0446
testosterone
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
higher levels
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DET2_SOLLC
257
6
29802
Swiss-Prot
Secretory Pathway (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into vector pTarget and expressed in COS-7 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rosati, F.; Bardazzi, I.; De Blasi, P.; Simi, L.; Scarpi, D.; Guarna, A.; Serio, M.; Racchi, M.L.; Danza, G.
5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes
J. Steroid Biochem. Mol. Biol.
96
287-299
2005
Arabidopsis thaliana, Solanum lycopersicum (Q5K2N1), Solanum lycopersicum
Manually annotated by BRENDA team