Information on EC 1.3.1.108 - caffeoyl-CoA reductase

electron-bifurcating caffeoyl-CoA reductase

hydrocaffeoyl-CoA:NAD+, ferredoxin oxidoreductase

3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster

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3-(3,4-dihydroxyphenyl)propanoyl-CoA:NAD+, ferredoxin oxidoreductase

The enzyme, characterized from the bacterium Acetobacterium woodii, contains two [4Fe-4S] clusters and FAD. The enzyme couples the endergonic ferredoxin reduction with NADH as reductant to the exergonic reduction of caffeoyl-CoA with the same reductant. It uses the mechanism of electron bifurcation to overcome the steep energy barrier in ferredoxin reduction. It also reduces 4-coumaroyl-CoA and feruloyl-CoA.

(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster

3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster

4 entries

4-coumaroyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster

? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster

feruloyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster

? + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster

(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster

3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster

FAD

the enzyme contains 4 mol of FAD per of enzyme. The CarCDE complex requires FAD for catalytic activity. No activity can be detected when FAD or FMN (0.25 mM) are used alone as electron acceptor, but together with caffeyl-CoA, they are completely reduced with NADH as reductant. Caffeyl-CoA reduction is stimulated by 26- and 12fold with FAD and FMN, respectively. The Km value for both FAD and FMN is 0.1 mM. Thus, caffeyl-CoA and flavins are reduced simultaneously

FMN

no activity can be detected when FAD or FMN (0.25 mM) are used alone as electron acceptor, but together with caffeyl-CoA, they are completely reduced with NADH as reductant. Caffeyl-CoA reduction is stimulated by 26- and 12-fold with FAD and FMN, respectively. The Km value for both FAD and FMN is 0.1 mM. Thus, caffeyl-CoA and flavins are reduced simultaneously

[4Fe-4S]-center

the enzyme contains 2 [4Fe-4S] clusters. It contains 9 mol of iron, and 9 mol of acid-labile sulfur per mol of enzyme

7.5

caffeoyl-CoA-dependent methyl viologen oxidation assay

6 - 9

pH 6.0: about 30% of maximal activity, pH 9.0: about 30% of maximal activity

30

caffeoyl-CoA-dependent methyl viologen oxidation assay

H6LGM6: caffeoyl-CoA reductase CarC, H6LGM7: electron transfer flavoprotein beta subunit CarD, H6LGM8: electron transfer flavoprotein alpha subunit CarE

Acetobacterium woodii DAM 1030

UniProt

H6LGM6: caffeoyl-CoA reductase CarC, H6LGM7: electron transfer flavoprotein beta subunit CarD, H6LGM8: electron transfer flavoprotein alpha subunit CarE

UniProt

metabolism

Acetobacterium woodii DSM 1030

6fah, download, 3D-view

SCOPe (6fah)

CATH (6fah)

H6LGM6, H6LGM7, H6LGM8

28200

1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE

350000

caffeoyl-CoA reductase and the electron transfer flavoprotein CarDE form a stable complex

41400

1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE

42500

1 * 42500 (electron transfer flavoprotein alpha subunit CarE) + 1 * 41400 (caffeoyl-CoA reductase CarC) + 1 * 28200 (electron transfer flavoprotein beta subunit CarD), SDS-PAGE

homotrimer

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