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Information on EC 1.23.1.4 - (-)-lariciresinol reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FVQ6

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IUBMB Comments
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (-)-pinoresinol [EC 1.23.1.3, (-)-pinoresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) and Linum corymbulosum .
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Arabidopsis thaliana
UNIPROT: Q9FVQ6
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
()-pinoresinol-()-lariciresinol reductase, bi-functional pinoresinol/lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase 1, LuPLR1, More, pinoresinol lariciresinol reductase, pinoresinol reductase, pinoresinol reductase 1, pinoresinol-lariciresinol reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
bi-functional pinoresinol/lariciresinol reductase
-
pinoresinol reductase
-
pinoresinol reductase 1
-
additional information
cf. EC 1.23.1.3
SYSTEMATIC NAME
IUBMB Comments
(+)-secoisolariciresinol:NADPH+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (-)-pinoresinol [EC 1.23.1.3, (-)-pinoresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) [1] and Linum corymbulosum [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-lariciresinol + NADPH + H+
(+)-secoisolariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1. The pinoresinol reductase shows only weak activity toward lariciresinol
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(-)-lariciresinol + NADPH + H+
(+)-secoisolariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
(-)-pinoresinol is successively converted into (-)-lariciresinol and (+)-secoisolariciresinol by isozyme PrR1. The pinoresinol reductase shows only weak activity toward lariciresinol
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
PrR1 is highly expressed in lignified inflorescence stem tissue
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the loss-of-function mutant of PrR1 shows, in addition to elevated levels of pinoresinol, significantly decreased lignin content and a slightly altered lignin structure with lower abundance of cinnamyl alcohol end groups. Stimulated Raman scattering (SRS) microscopy analysis indicates that the lignin content of the prr1-1 loss-of-function mutant is similar to that of wild-type plants in xylem cells, which exhibit a normal phenotype, but is reduced in the fiber cells. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin. The PrR1 isozyme mutation alters the expression level of diverse genes, overview
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
physiological function
pinoresinol reductase (PrR) catalyzes the conversion of the lignan (-)-pinoresinol to (-)-lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. The enzyme is responsible for the synthesis of the major enantiomer (+)-secoisolariciresinol, it is strongly expressed in the seed coats of developing flax seed. Association of the lignan biosynthetic enzyme encoded by PrR1 with secondary cell wall biosynthesis in fiber cells. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46. PrR1 plays a role in lignan biosynthesis in aerial tissue
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PILR1_ARATH
317
1
35549
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
the prr1-1 knock-out mutant SALK_058467 contains a T-DNA insertion within an exonic region of the PrR1 gene. The concentration of the lignan, pinoresinol, is 4.8fold higher in the loss-of-function prr1 mutant than in control plants. Impact of loss of function of PrR1 on the cellular distribution of lignin
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhao, Q.; Zeng, Y.; Yin, Y.; Pu, Y.; Jackson, L.A.; Engle, N.L.; Martin, M.Z.; Tschaplinski, T.J.; Ding, S.Y.; Ragauskas, A.J.; Dixon, R.A.
Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis
Phytochemistry
112
170-178
2015
Arabidopsis thaliana (Q9FVQ6)
Manually annotated by BRENDA team