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Information on EC 1.23.1.3 - (-)-pinoresinol reductase and Organism(s) Thuja plicata and UniProt Accession Q9LD14

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EC Tree
     1 Oxidoreductases
         1.23 Reducing C-O-C group as acceptor
             1.23.1 With NADH or NADPH as donor
                1.23.1.3 (-)-pinoresinol reductase
IUBMB Comments
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that usually further reduces the product to (+)-secoisolariciresinol [EC 1.23.1.4, (-)-lariciresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) , Linum perenne (perennial flax) and Arabidopsis thaliana (thale cress) .
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This record set is specific for:
Thuja plicata
UNIPROT: Q9LD14
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Word Map
The taxonomic range for the selected organisms is: Thuja plicata
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
luplr1, pinoresinol lariciresinol reductase, plr-lu1, bifunctional pinoresinol-lariciresinol reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pinoresinol-lariciresinol reductase
-
(–)-pinoresinol-(–)-lariciresinol reductase
-
-
pinoresinol-lariciresinol reductase
-
-
pinoresinol/lariciresinol reductase
-
-
SYSTEMATIC NAME
IUBMB Comments
(-)-lariciresinol:NADP+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that usually further reduces the product to (+)-secoisolariciresinol [EC 1.23.1.4, (-)-lariciresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) [1], Linum perenne (perennial flax) [2] and Arabidopsis thaliana (thale cress) [3].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-pinoresinol + NADPH + H+
(-)-lariciresinol + NADP+
show the reaction diagram
-
-
-
?
(-)-lariciresinol + NADPH + H+
(+)-secoisolariciresinol + NADP+
show the reaction diagram
-
-
-
?
(-)-pinoresinol + NADPH + H+
(-)-lariciresinol + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PILR1_THUPL
313
0
35580
Swiss-Prot
other Location (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M MES, pH 6.2, 0.1 M NaCl, and 0.1 M calcium acetate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
POROS 20 HQ column chromatography and ADP-agarose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834-DE3 cells
expressed in Escherichia coli Nova Blue cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fujita, M.; Gang, D.R.; Davin, L.B.; Lewis, N.G.
Recombinant pinoresinol-lariciresinol reductases from western red cedar (Thuja plicata) catalyze opposite enantiospecific conversions
J. Biol. Chem.
274
618-627
1999
Thuja plicata
Manually annotated by BRENDA team
Min, T.; Kasahara, H.; Bedgar, D.L.; Youn, B.; Lawrence, P.K.; Gang, D.R.; Halls, S.C.; Park, H.; Hilsenbeck, J.L.; Davin, L.B.; Lewis, N.G.; Kang, C.
Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases
J. Biol. Chem.
278
50714-50723
2003
Thuja plicata, Thuja plicata (Q9LD14)
Manually annotated by BRENDA team