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Information on EC 1.23.1.2 - (+)-lariciresinol reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SVP6

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IUBMB Comments
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (+)-pinoresinol [EC 1.23.1.1, (+)-pinoresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) , Linum perenne (perennial flax) and Linum corymbulosum .
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Arabidopsis thaliana
UNIPROT: Q9SVP6
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
(+)-lariciresinol reductase, (+)-pinoresinol-(+)-lariciresinol reductase, (+)-pinoresinol/(+)-lariciresinol reductase, bi-functional pinoresinol/lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase 2, LuPLR2, More, pinoresinol reductase, pinoresinol reductase 2, pinoresinol-lariciresinol reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
bi-functional pinoresinol/lariciresinol reductase
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pinoresinol reductase
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pinoresinol reductase 2
-
additional information
cf. EC 1.23.1.1
SYSTEMATIC NAME
IUBMB Comments
(–)-secoisolariciresinol:NADP+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (+)-pinoresinol [EC 1.23.1.1, (+)-pinoresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) [3], Linum perenne (perennial flax) [5] and Linum corymbulosum [6].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-lariciresinol + NADPH + H+
(-)-secoisolariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.1. The pinoresinol reductase shows only weak activity toward lariciresinol
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-lariciresinol + NADPH + H+
(-)-secoisolariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.1. The pinoresinol reductase shows only weak activity toward lariciresinol
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
PrR2 expression is barely detectable in stems
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
physiological function
pinoresinol reductase catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. The isozyme PrR2 is responsible for the synthesis of the enantiomer (-)-secoisolariciresinol
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PILR2_ARATH
317
0
35415
Swiss-Prot
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
isozyme mutant prr2-1, SALK_123621
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene PrP2, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR2 expression clusters with a different set of genes, not involved in secondary cell wall biosynthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhao, Q.; Zeng, Y.; Yin, Y.; Pu, Y.; Jackson, L.A.; Engle, N.L.; Martin, M.Z.; Tschaplinski, T.J.; Ding, S.Y.; Ragauskas, A.J.; Dixon, R.A.
Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis
Phytochemistry
112
170-178
2015
Arabidopsis thaliana (Q9SVP6)
Manually annotated by BRENDA team