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Information on EC 1.23.1.1 - (+)-pinoresinol reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FVQ6

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EC Tree
     1 Oxidoreductases
         1.23 Reducing C-O-C group as acceptor
             1.23.1 With NADH or NADPH as donor
                1.23.1.1 (+)-pinoresinol reductase
IUBMB Comments
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that further reduces the product to the lignan (-)-secoisolariciresinol [EC 1.23.1.2, (+)-lariciresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) , Linum perenne (perennial flax) and Linum corymbulosum . The 4-pro-R hydrogen of NADH is transferred to the 7-pro-R position of lariciresinol .
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Arabidopsis thaliana
UNIPROT: Q9FVQ6
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
pinoresinol-lariciresinol reductase, pinoresinol/lariciresinol reductase, pinoresinol reductase, luplr2, plr-lc1, plr-la1, plr-lu2, (+)-pinoresinol/(+)-lariciresinol reductase, (+)-pinoresinol-(+)-lariciresinol reductase, rr-pinoresinol-rr-lariciresinol reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
bi-functional pinoresinol/lariciresinol reductase
-
pinoresinol reductase
-
pinoresinol reductase 2
-
additional information
cf. EC 1.23.1.2
SYSTEMATIC NAME
IUBMB Comments
(+)-lariciresinol:NADP+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that further reduces the product to the lignan (-)-secoisolariciresinol [EC 1.23.1.2, (+)-lariciresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) [3], Linum perenne (perennial flax) [5] and Linum corymbulosum [6]. The 4-pro-R hydrogen of NADH is transferred to the 7-pro-R position of lariciresinol [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-pinoresinol + NADPH + H+
(+)-lariciresinol + NADP+
show the reaction diagram
efficient substrate
-
-
?
(+)-pinoresinol + NADPH + H+
(+)-lariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-pinoresinol + NADPH + H+
(+)-lariciresinol + NADP+
show the reaction diagram
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
(+)-pinoresinol
at pH 7.5 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.8
(+)-pinoresinol
at pH 7.5 and 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Columbia
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcript levels in root are higher than those in stem
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the enantiomeric control in lignan biosynthesis
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
physiological function
pinoresinol reductase catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. The isozyme PrR2 is responsible for the synthesis of the enantiomer (-)-secoisolariciresinol
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PILR1_ARATH
317
1
35549
Swiss-Prot
other Location (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-Bind resin affinity column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) cells
gene PrP2, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR2 expression clusters with a different set of genes, not involved in secondary cell wall biosynthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakatsubo, T.; Mizutani, M.; Suzuki, S.; Hattori, T.; Umezawa, T.
Characterization of Arabidopsis thaliana pinoresinol reductase, a new type of enzyme involved in lignan biosynthesis
J. Biol. Chem.
283
15550-15557
2008
Arabidopsis thaliana (Q9FVQ6), Arabidopsis thaliana
Manually annotated by BRENDA team
Zhao, Q.; Zeng, Y.; Yin, Y.; Pu, Y.; Jackson, L.A.; Engle, N.L.; Martin, M.Z.; Tschaplinski, T.J.; Ding, S.Y.; Ragauskas, A.J.; Dixon, R.A.
Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis
Phytochemistry
112
170-178
2015
Arabidopsis thaliana (Q9SVP6), Arabidopsis thaliana
Manually annotated by BRENDA team