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Information on EC 1.21.3.8 - cannabidiolic acid synthase for references in articles please use BRENDA:EC1.21.3.8
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EC Tree
IUBMB Comments Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
The expected taxonomic range for this enzyme is: Cannabis sativa
Synonyms
cbda synthase, cbdas,
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cannabidiolic-acid synthase
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CBDA synthase
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cannabigerolate + O2 = cannabidiolate + H2O2
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cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
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cannabigerolate + O2
cannabidiolate + H2O2
cannabinerolate + O2
cannabidiolate + H2O2
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?
additional information
?
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the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH
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cannabigerolate + O2
cannabidiolate + H2O2
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?
cannabigerolate + O2
cannabidiolate + H2O2
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ir
cannabigerolate + O2
cannabidiolate + H2O2
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?
cannabigerolate + O2
cannabidiolate + H2O2
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best substrate
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?
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cannabigerolate + O2
cannabidiolate + H2O2
cannabigerolate + O2
cannabidiolate + H2O2
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?
cannabigerolate + O2
cannabidiolate + H2O2
A0A0E3XIC7
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?
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FAD
the enzyme has a covalently bound flavin
additional information
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the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN
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additional information
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the enzyme does not require metal ions
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Hg2+
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complete inhibition at 2 mM
additional information
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EDTA has little influence on the enzyme activity at concentrations up to 5 mM
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0.137
cannabigerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.206
cannabinerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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0.19
cannabigerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.03
cannabinerolate
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in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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0.175
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enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
9
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after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
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4 - 6
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half-maximal activities at pH values around 4.0 and 6.0
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6.1
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isoelectric focusing
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UniProt
brenda
CBDA and Mexican strains
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brenda
CBDA strain
UniProt
brenda
cultivars Skunk #1 and Carmen
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brenda
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brenda
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CBDAS_CANSA
544
1
62237
Swiss-Prot
A0A084G6X6_9PEZI
125
0
13506
TrEMBL
A0A2P6R0P3_ROSCH
92
0
10673
TrEMBL
A0A2P6Q6N0_ROSCH
189
2
21163
TrEMBL
A0A0E3XIC7_CANSA
544
0
62168
TrEMBL
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58863
x * 58863, deduced from amino acid sequence
62000
x * 62000, SDS-PAGE
74000
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1 * 74000, SDS-PAGE
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?
x * 58863, deduced from amino acid sequence; x * 62000, SDS-PAGE
monomer
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1 * 74000, SDS-PAGE
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-20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization
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4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity
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ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography
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hydroxylapatite column chromatography
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expressed in Sf9 insect cells
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Taura, F.; Sirikantaramas, S.; Shoyama, Y.; Yoshikai, K.; Shoyama, Y.; Morimoto, S.
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa
FEBS Lett.
581
2929-2934
2007
Cannabis sativa (A6P6V9), Cannabis sativa
brenda
Taura, F.; Morimoto, S.; Shoyama, Y.
Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid
J. Biol. Chem.
271
17411-17416
1996
Cannabis sativa
brenda
Weiblen, G.D.; Wenger, J.P.; Craft, K.J.; ElSohly, M.A.; Mehmedic, Z.; Treiber, E.L.; Marks, M.D.
Gene duplication and divergence affecting drug content in Cannabis sativa
New Phytol.
208
1241-1250
2015
Cannabis sativa
brenda
Onofri, C.; de Meijer, E.P.M.; Mandolino, G.
Sequence heterogeneity of cannabidiolic- and tetrahydrocannabinolic acid-synthase in Cannabis sativa L. and its relationship with chemical phenotype
Phytochemistry
116
57-68
2015
Cannabis sativa (A0A0E3XIC7), Cannabis sativa
brenda
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1.21.3.8 cannabidiolic acid synthase
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