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Information on EC 1.20.2.1 - arsenate reductase (cytochrome c) and Organism(s) Pseudorhizobium banfieldiae and UniProt Accession Q6VAL8

for references in articles please use BRENDA:EC1.20.2.1
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IUBMB Comments
A molybdoprotein containing iron-sulfur clusters. Isolated from alpha-proteobacteria. Unlike EC 1.20.9.1, arsenate reductase (azurin), it does not use azurin as acceptor.
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This record set is specific for:
Pseudorhizobium banfieldiae
UNIPROT: Q6VAL8
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The taxonomic range for the selected organisms is: Pseudorhizobium banfieldiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
+
2
oxidized cytochrome c
=
+
2
reduced cytochrome c
+
2
Synonyms
as(iii) oxidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
arsenite:cytochrome c oxidoreductase
A molybdoprotein containing iron-sulfur clusters. Isolated from alpha-proteobacteria. Unlike EC 1.20.9.1, arsenate reductase (azurin), it does not use azurin as acceptor.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
arsenite + H2O + 2,6-dichlorophenolindophenol
arsenate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
molybdenum cofactor
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
protein contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.061
arsenite
pH 5.5, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.6
arsenite
pH 5.5, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
an aroA disruption mutant does not display arsenite oxidation activity, fails to grow chemolithoautotrophically with arsenite as the electron donor, but is able to grow in presence of 5 mM arsenite
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q6VAL8_9HYPH
845
0
93272
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
219000
gel filtration
98000
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
2 * 98000, subunit aroA, plus 2 * 14000, subunit aroB, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Santini, J.; Vanden Hoven, R.
Molybdenum-containing arsenite oxidase of the chemolithoautotrophic arsenite oxidizer NT-26
J. Bacteriol.
186
1614-1619
2004
arsenite-oxidising bacterium (Q6VAL9), arsenite-oxidising bacterium NT-26 (Q6VAL9), Pseudorhizobium banfieldiae (Q6VAL8)
Manually annotated by BRENDA team