Information on EC 1.2.3.8 - pyridoxal oxidase

for references in articles please use BRENDA:EC1.2.3.8
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The expected taxonomic range for this enzyme is: Drosophila melanogaster

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EC NUMBER
COMMENTARY hide
1.2.3.8
-
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RECOMMENDED NAME
GeneOntology No.
pyridoxal oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pyridoxal + H2O + O2 = 4-pyridoxate + (?)
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Vitamin B6 metabolism
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-
Metabolic pathways
-
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Microbial metabolism in diverse environments
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-
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SYSTEMATIC NAME
IUBMB Comments
pyridoxal:oxygen 4-oxidoreductase
A molybdenum protein.
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CAS REGISTRY NUMBER
COMMENTARY hide
76415-81-1
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4,5-trimethoxybenzaldehyde + H2O + O2
2,4,5-trimethoxybenzoate + ?
show the reaction diagram
3,4,5-trimethoxybenzaldehyde + H2O + O2
3,4,5-trimethoxybenzoate + ?
show the reaction diagram
-
-
-
-
?
3,7-dimethyl-2,6-octadienal + H2O + O2
3,7-dimethyl-2,6-octadienoate + ?
show the reaction diagram
-
-
-
-
?
3,7-dimethyl-6-octenal + H2O + O2
3,7-dimethyl-6-octenoate + ?
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + H2O + O2
3-hydroxybenzoate + ?
show the reaction diagram
-
-
-
-
?
3-methoxy-4-hydroxybenzaldehyde + H2O + O2
3-methoxy-4-hydroxybenzoate + ?
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + H2O + O2
4-hydroxybenzoate + ?
show the reaction diagram
-
-
-
-
?
pyridoxal + H2O + O2
4-pyridoxate + ?
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxal + H2O + O2
4-pyridoxate + ?
show the reaction diagram
Q9VF53
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
molybdopterin
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Molybdenum
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.57 - 0.85
2,4,5-trimethoxybenzaldehyde
0.028
pyridoxal
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
immediately after extraction the optimum is pH 8.0, after 5 h it is pH 7.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
distribution pattern in imaginal wing dics for a series of strains of Drosophila melanogaster heterozygous for different Minute mutations. The amount of enzyme activity is roughly correlated with the expressivity in the Minute mutation. M(3)w124Sb63bmwh/TM2, which is a strong Minute, has a high level of activity in ist imaginal discs
Manually annotated by BRENDA team
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
unstable above
390445
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
activity in crude extract is increased after 1 min to 130-140% of the initial unheated activity. 110% after 5 min, 25% of the original activity after 30 min
60
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considerable denaturation
additional information
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activity is increased during the first 4-8 h following extraction when the extract is held at 4�C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superose 12 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli TP1000 cells
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LINKS TO OTHER DATABASES (specific for EC-Number 1.2.3.8)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)