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Information on EC 1.2.3.4 - oxalate oxidase and Organism(s) Triticum aestivum and UniProt Accession P15290
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1.2.3.4 oxalate oxidaseIUBMB Comments
Contains Mn2+ as a cofactor. The enzyme is not a flavoprotein as had been thought .
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Word Map
- 1.2.3.4
- wheat
- barley
- sclerotinia
- apoplastic
-
powdery
- mildew
-
pathogenesis-related
- sclerotiorum
- graminis
- hyperoxaluria
- blumeria
-
hordei
- erysiphe
- nodorum
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bicupins
- dentata
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castanea
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oxalate-degrading
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berk
- alkylamine
- septoria
- medicine
- analysis
- industry
- synthesis
- agriculture
The taxonomic range for the selected organisms is: Triticum aestivum
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
oxalate oxidase, germin, germin-like protein, oxo-g, gl-oxo, germin gf-2.8, oxalic acid oxidase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
aero-oxalo dehydrogenase
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Germin
Germin GF-2.8
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Germin GF-3.8
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oxalic acid oxidase
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Germin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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-
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
oxalate:oxygen oxidoreductase
Contains Mn2+ as a cofactor. The enzyme is not a flavoprotein as had been thought [3].
CAS REGISTRY NUMBER
COMMENTARY
9031-79-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Sodium periodate
the oxalate oxidase activity of the recombinant enzyme is 7fold increased by treatment with sodium periodate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000002
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gl-OXO activity in homogenates of embryos 12 days after fertilisation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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after 24 h of induction on auxin-containing medium gl-OXO activity is readily detectable within the embryo, particularly within the coleorhiza and on the residual cells of the suspensor
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When embryos are cultured together with endosperms (endosperm-supported culture, ES), the percentage of callus induction is significantly lower than that when embryos are cultured in the absence of endosperm (non-endosperm-supported culture, NES). The activity of oxalate oxsidase in the callus of ES culture is higher than that in the callus of NES culture, suggesting that the activity of oxalate oxidase may be a parameter for selection of calli with potential for plantlet regeneration
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
transgenic soybean plants overexpressing an oxalate oxidase gene from wheat show enhanced resistance to sclerotina stem rot. They display significantly reduced lesion sizes, i.e. by 58.71-82.73% reduction of lesion length in a detached stem assay (T3 and T4 generations) and 76.67-82.0% reduction of lesion area in a detached leaf assay (T4 generation). The transgenic plants also show increased tolerance to the externally applied oxalic acid (60 mM) relative to the controls. Decreased oxalic acid content and increased hydrogen peroxide (H2O2) levels are also observed in the transgenic leaves after Scerotina sclerotiorum inoculation
metabolism
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the enzyme is capable of stimulating the ester-linked diferulic acid formation. The enzyme is capable of modifying the metabolism of ester-linked ferulates in cell walls of wheat shoots by promoting the peroxidase action via supply of hydrogen peroxide
physiological function
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transgenic Arabidopsis lines that express the enzyme exhibit enhanced resistance to oxalate exposure and Sclerotinia sclerotiorum infection
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GER2_WHEAT
224
1
23612
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
S49A
the mutant retains essentially full enzyme activity but exhibits altered protein-protein interactions
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Zea mays, expression in Zea mays leads to increased resistance against Ostrinia nubilalis
into the pBI121 binary vector, the new construct pBI-OxO is transformed into Escherichia coli and then to Agrobacterium tumefaciens LBA4404, subsequently leaf discs of Nicotiana tabacum plants are transformed by culturing with Agrobacterium tumefaciens
expressed in Brassica napus cultivar 84039M, the expression leads to considerably increased OXO activity and enhanced resistance to Sclerotinia sclerotiorum
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
overexpression of oxalate oxidase provides a new strategy for induction of antioxidative defense system against cellular ROS accumulation and for protection of crops from stress injury
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cassland, P.; Larsson, S.; Nilvebrant, N.O.; Jonsson, L.J.
Heterologous expression of barley and wheat oxalate oxidase in an E. coli trxB gor double mutant
J. Biotechnol.
109
53-62
2004
Hordeum vulgare, Triticum aestivum
Hu, X.; Bidney, D.L.; Yalpani, N.; Duvick, J.P.; Crasta, O.; Folkerts, O.; Lu, G.
Overexpression of a gene encoding hydrogen peroxide-generating oxalate oxidase evokes defense responses in sunflower
Plant Physiol.
133
170-181
2003
Triticum aestivum
Caliskan, M.; Turet, M.; Cuming, A.C.
Formation of wheat (Triticum aestivum L.) embryogenic callus involves peroxide-generating germin-like oxalate oxidase
Planta
219
132-140
2004
Triticum aestivum
Chen, J.; Yue, R.; Xu, H.; Chen, X.
Study on plant regeneration of wheat mature embryos under endosperm-supported culture
Agric. Sci. China
5
572-578
2006
Triticum aestivum
-
Betsche, T.; Fretzdorff, B.
Biodegradation of oxalic acid from spinach using cereal radicles
J. Agric. Food Chem.
53
9751-9758
2005
Avena sativa, Hordeum vulgare, Secale cereale, Triticum aestivum, Zea mays, Triticum spelta
Pan, H.Y.; Whittaker, M.M.; Bouveret, R.; Berna, A.; Bernier, F.; Whittaker, J.W.
Characterization of wheat germin (oxalate oxidase) expressed by Pichia pastoris
Biochem. Biophys. Res. Commun.
356
925-929
2007
Triticum aestivum (P15290), Triticum aestivum
Mao, J.; Burt, A.J.; Ramputh, A.I.; Simmonds, J.; Cass, L.; Hubbard, K.; Miller, S.; Altosaar, I.; Arnason, J.T.
Diverted secondary metabolism and improved resistance to European corn borer (Ostrinia nubilalis) in maize (Zea mays L.) transformed with wheat oxalate oxidase
J. Agric. Food Chem.
55
2582-2589
2007
Triticum aestivum (P15290), Triticum aestivum
Zou, Q.J.; Liu, S.Y.; Dong, X.Y.; Bi, Y.H.; Cao, Y.C.; Xu, Q.; Zhao, Y.D.; Chen, H.
In vivo measurements of changes in pH triggered by oxalic acid in leaf tissue of transgenic oilseed rape
Phytochem. Anal.
18
341-346
2007
Triticum aestivum
Welch, A.J.; Stipanovic, A.J.; Maynard, C.A.; Powell, W.A.
The effects of oxalic acid on transgenic Castanea dentata callus tissue expressing oxalate oxidase
Plant Sci.
172
488-496
2007
Triticum aestivum
Dong, X.; Ji, R.; Guo, X.; Foster, S.J.; Chen, H.; Dong, C.; Liu, Y.; Hu, Q.; Liu, S.
Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus)
Planta
228
331-340
2008
Triticum aestivum
Wan, X.; Tan, J.; Lu, S.; Lin, C.; Hu, Y.; Guo, Z.
Increased tolerance to oxidative stress in transgenic tobacco expressing a wheat oxalate oxidase gene via induction of antioxidant enzymes is mediated by H2O2
Physiol. Plant.
136
30-44
2009
Triticum aestivum (P15290), Triticum aestivum
Wakabayashi, K.; Soga, K.; Hoson, T.
Cell wall oxalate oxidase modifies the ferulate metabolism in cell walls of wheat shoots
J. Plant Physiol.
168
1997-2000
2011
Triticum aestivum
Wei, F.; Hu, J.; Yang, Y.; Hao, Z.; Wu, R.; Tian, B.; Cao, G.; Zang, X.
Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response
J. Integr. Agric.
14
2565-2573
2015
Triticum aestivum
-
Yang, X.; Yang, J.; Wang, Y.; He, H.; Niu, L.; Guo, D.; Xing, G.; Zhao, Q.; Zhong, X.; Sui, L.; Li, Q.; Dong, Y.
Enhanced resistance to sclerotinia stem rot in transgenic soybean that overexpresses a wheat oxalate oxidase
Transgenic Res.
28
103-114
2019
Triticum aestivum (P15290), Triticum aestivum
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