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Enzyme Nomenclature
Information on EC 1.2.1.96 - 4-hydroxybenzaldehyde dehydrogenase (NADP+)
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1.2.1.96 4-hydroxybenzaldehyde dehydrogenase (NADP+)IUBMB Comments
Involved in the aerobic pathway for degradation of toluene, 4-methylphenol, and 2,4-xylenol by several Pseudomonas strains. The enzyme is also active with 4-hydroxy-3-methylbenzaldehyde. cf. EC 1.2.1.64, 4-hydroxybenzaldehyde dehydrogenase (NAD+).
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
p-hydroxybenzaldehyde dehydrogenase
PchA
PHBDD
PHBZ
p-hydroxybenzaldehyde dehydrogenase
-
-
ambiguous
-
PchA
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4-hydroxybenzaldehyde + NADP+ + H2O = 4-hydroxybenzoate + NADPH + 2 H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dehydrogenation
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzaldehyde:NADP+ oxidoreductase
Involved in the aerobic pathway for degradation of toluene, 4-methylphenol, and 2,4-xylenol by several Pseudomonas strains. The enzyme is also active with 4-hydroxy-3-methylbenzaldehyde. cf. EC 1.2.1.64, 4-hydroxybenzaldehyde dehydrogenase (NAD+).
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
4-hydroxy-3-methylbenzoic acid + NADH + H+
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
4-hydroxy-3-methylbenzoic acid + NADH + H+
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
4-hydroxy-3-methylbenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoic acid + NADH + H+
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoic acid + NADH + H+
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of toluene
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of toluene
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of p-cresol
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
-
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of toluene
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of toluene
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
-
involved in the degradation pathway of p-cresol
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00636
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
0.02055
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
0.00357
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
0.01311
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0017
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
0.071
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
0.0017
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
0.26
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.083
4-hydroxy-3-methylbenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
11.16
4-hydroxy-3-methylbenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
0.13
4-hydroxybenzaldehyde
with NAD+ as cosubstrate, at pH 8.0 and 30°C
72.83
4-hydroxybenzaldehyde
with NADP+ as cosubstrate, at pH 8.0 and 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
high expression in normal hairy root, activity is 15fold lower in green hairy root
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A2S6S7W6_9PROT
460
0
51704
TrEMBL
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cronin, C.N.; Kim, J.; Fuller, J.H.; Zhang, X.; McIntire, W.S.
Organization and sequences of p-hydroxybenzaldehyde dehydrogenase and other plasmid-encoded genes for early enzymes of the p-cresol degradative pathway in Pseudomonas putida NCIMB 9866 and 9869
DNA Seq.
10
7-17
1999
Pseudomonas putida
brenda
Whited, G.M.; Gibson, D.T.
Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendocina KR1
J. Bacteriol.
173
3017-3020
1991
Pseudomonas mendocina, Pseudomonas mendocina KR1
brenda
Chen, Y.F.; Chao, H.; Zhou, N.Y.
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866
Appl. Microbiol. Biotechnol.
98
1349-1356
2014
Pseudomonas putida (Q59702), Pseudomonas putida NCIMB 9866 (Q59702)
-
brenda
Miller Jr, E.S.; Peretti, S.W.
Bioconversion of toluene to p-hydroxybenzoate via the construction and characterization of a recombinant Pseudomonas putida
Green Chem.
1
143-152
1999
Pseudomonas putida, Pseudomonas putida EM2839
-
brenda
Mukherjee, C.; Samanta, T.; Mitra, A.
Redirection of metabolite biosynthesis from hydroxybenzoates to volatile terpenoids in green hairy roots of Daucus carota
Planta
243
305-320
2016
Daucus carota
brenda
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