Information on EC 1.2.1.81 - sulfoacetaldehyde dehydrogenase (acylating)

for references in articles please use BRENDA:EC1.2.1.81
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The expected taxonomic range for this enzyme is: Cupriavidus necator

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EC NUMBER
COMMENTARY hide
1.2.1.81
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RECOMMENDED NAME
GeneOntology No.
sulfoacetaldehyde dehydrogenase (acylating)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-sulfoacetaldehyde + CoA + NADP+ = sulfoacetyl-CoA + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfoacetate degradation
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SYSTEMATIC NAME
IUBMB Comments
2-sulfoacetaldehyde:NADP+ oxidoreductase (CoA-acetylating)
The enzyme is involved in degradation of sulfoacetate. In this pathway the reaction is catalysed in the reverse direction. The enzyme is specific for sulfoacetaldehyde and NADP+.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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UniProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-sulfoacetaldehyde + CoA + NADP+
sulfoacetyl-CoA + NADPH + H+
show the reaction diagram
sulfoacetyl-CoA + NADPH + H+
2-sulfoacetaldehyde + CoA + NADP+
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfoacetyl-CoA + NADPH + H+
2-sulfoacetaldehyde + CoA + NADP+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
specific for NADP+, no activity with NAD+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
2-sulfoacetaldehyde
pH 9.0, temperature not specified in the publication
0.102
CoA
pH 9.0, temperature not specified in the publication
0.064
NADP+
pH 9.0, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
2 * 52000, SDS-PAGE
115000
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or -18°C, 1 week, 30% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction
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LINKS TO OTHER DATABASES (specific for EC-Number 1.2.1.81)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)