Information on EC 1.2.1.78 - 2-formylbenzoate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.78
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RECOMMENDED NAME
GeneOntology No.
2-formylbenzoate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-formylbenzoate + NAD+ + H2O = o-phthalic acid + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Polycyclic aromatic hydrocarbon degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
2-formylbenzoate:NAD+ oxidoreductase
The enzyme is involved in phenanthrene degradation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-carboxybenzaldehyde + NAD+ + H2O
o-phthalic acid + NADH + H+
show the reaction diagram
2-nitrobenzaldehyde + NAD+ + H2O
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-carboxybenzaldehyde + NAD+ + H2O
o-phthalic acid + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-naphthoaldehyde
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0.2 mM, 34.6% inhibition
4-carboxybenzaldehyde
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0.2 mM, 38.5% inhibition
acetaldehyde
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0.2 mM, 34.6% inhibition
Ag+
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1 mM, complete inhibition
Ca2+
10 mM, about 30% inhibition
Co2+
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1 mM, 40.2% inhibition
Cu2+
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1 mM, complete inhibition
EDTA
10 mM, 28% inhibition
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid
10 mM, 14% inhibition
Hg2+
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1 mM, 17.5% inhibition
iso-phthalaldehyde
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0.2 mM, 23.8% inhibition
Mg2+
10 mM, about 30% inhibition
Mn2+
10 mM, about 30% inhibition
o-nitrobenzaldehyde
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0.2 mM, 58.1% inhibition
o-phthalaldehyde
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0.2 mM, 91.4% inhibition
p-chloromercuribenzoate
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1 mM, complete inhibition
p-dimethylamino benzaldehyde
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0.2 mM, 32.7% inhibition
propionaldehyde
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0.2 mM, 31.7% inhibition
Protocatechualdehyde
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0.2 mM, 23.1% inhibition
Salicylaldehyde
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0.2 mM, 23.1% inhibition
SDS
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1 mM, 44.3% inhibition
vertralaldehyde
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0.2 mM, 13.5% inhibition
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Zn2+
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1 mM, 71.7% inhibition
additional information
less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054 - 2.8
2-carboxybenzaldehyde
0.083 - 0.14
NAD+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
39
2-carboxybenzaldehyde
pH 9.4, 25C
48
2-nitrobenzaldehyde
pH 9.4, 25C
32
NAD+
pH 9.4, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.6
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pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
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4 * 41000, SDS-PAGE
53000
4 * 53000, SDS-PAGE
158000
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gel filtration
205000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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10 min, no loss of activity below 30C
40
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10 min, 25% loss of activity
50
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10 min, 94% loss of activity
60
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10 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, activity in phosphate buffer is labile, 1 mM DTT stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli