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Information on EC 1.2.1.51 - pyruvate dehydrogenase (NADP+) and Organism(s) Euglena gracilis and UniProt Accession Q94IN5

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IUBMB Comments
The Euglena enzyme can also use FAD or methyl viologen as acceptor, more slowly. The enzyme is inhibited by oxygen.
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This record set is specific for:
Euglena gracilis
UNIPROT: Q94IN5
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The taxonomic range for the selected organisms is: Euglena gracilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
hide
pyruvate
+
CoA
+
NADP+
=
acetyl-CoA
+
CO2
+
NADPH
+
H+
+
+
=
+
+
+
Synonyms
pyruvate dehydrogenase, pyruvate:nadp+ oxidoreductase, cppno, aquacobalamin reductase (nadph), more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pyruvate:NADP+ oxidoreductase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
pyruvate:NADP+ 2-oxidoreductase (CoA-acetylating)
The Euglena enzyme can also use FAD or methyl viologen as acceptor, more slowly. The enzyme is inhibited by oxygen.
CAS REGISTRY NUMBER
COMMENTARY hide
93389-35-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pyruvate + CoA + NADP+
acetyl-CoA + CO2 + NADPH
show the reaction diagram
-
-
-
r
2-oxobutyrate + CoA + NADP+
propionyl-CoA + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
3-hydroxypyruvate + CoA + NADP+
hydoxyacetyl-CoA + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
oxaloacetate + CoA + NADP+
? + CO2 + NADPH
show the reaction diagram
-
-
-
-
?
pyruvate + CoA + methyl viologen
acetyl-CoA + CO2 + reduced methyl viologen
show the reaction diagram
-
-
-
-
r
pyruvate + CoA + NADP+
acetyl-CoA + CO2 + NADPH
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyruvate + CoA + NADP+
acetyl-CoA + CO2 + NADPH
show the reaction diagram
-
-
-
r
pyruvate + CoA + NADP+
acetyl-CoA + CO2 + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
not NAD+
NADP+
thiamine diphosphate
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe
iron-sulfur cluster
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',5'-ADP
-
-
2',5'-ATP-ribose
-
-
CO2
-
product inhibition
Quinacrine
-
-
additional information
-
not arsenite
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
-
dependent on
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0066 - 0.0081
CoA
0.068
methyl viologen
-
-
0.028 - 0.03
NADP+
0.027 - 0.21
pyruvate
additional information
additional information
-
kinetic mechanism
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.93
-
3-hydroxypyruvate
14.3
-
pyruvate
2.36
-
2-oxobutyrate
2.6
-
oxaloacetate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PNO_EUGGR
1803
0
199821
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
195000
2 * 195000, calculation from sequence
166000
-
2 * 166000, SDS-PAGE
190000
-
2 * 190000, SDS-PAGE
309000
320000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 195000, calculation from sequence
dimer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is stabilized by its cofactor, thiamine diphosphate, in mitochondria of Euglena gracilis
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2 leads to rapid inactivation
-
288278, 288279, 288281, 288283
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Inui, H.; Yamaji, R.; Saidoh, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
Pyruvate:NADP+ oxidoreductase from Euglena gracilis: limited proteolysis of the enzyme with trypsin
Arch. Biochem. Biophys.
286
270-276
1991
Euglena gracilis
Manually annotated by BRENDA team
Inui, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
Pyruvate: NADP+ oxidoreductase from Euglena gracilis: mechanism of O2-inactivation of the enzyme and its stability in the aerobe
Arch. Biochem. Biophys.
280
292-298
1990
Euglena gracilis
Manually annotated by BRENDA team
Inui, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
Pyruvate: NADP+ oxidoreductase from Euglena gracilis: the kinetic properties of the enzyme
Arch. Biochem. Biophys.
274
434-442
1989
Euglena gracilis
Manually annotated by BRENDA team
Inui, H.; Ono, K.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
Purification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis
J. Biol. Chem.
262
9130-9135
1987
Euglena gracilis
Manually annotated by BRENDA team
Inui, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
The physiological role of oxygen-sensitive pyruvate dehydrogenase in mitochondrial fatty acid synthesis in Euglena gracilis
Arch. Biochem. Biophys.
237
423-429
1985
Euglena gracilis
Manually annotated by BRENDA team
Inui, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
Occurrence of oxygen-sensitive, NADP+-dependent pyruvate dehydrogenase in mitochondria of Euglena gracilis
J. Biochem.
96
931-934
1984
Euglena gracilis
Manually annotated by BRENDA team
Nakazawa, M.; Inui, H.; Yamaji, R.; Yamamoto, T.; Takenaka, S.; Ueda, M.; Nakano, Y.; Miyatake, K.
The origin of pyruvate: NADP oxidoreductase in mitochondria of Euglena gracilis
FEBS Lett.
479
155-156
2000
Euglena gracilis (Q94IN5)
Manually annotated by BRENDA team
Lochmeyer, C.; Fuchs, G.
NADP+-specific 2-oxoglutarate dehydrogenase in denitrifying Pseudomonas species
Arch. Microbiol.
153
226-229
1990
Euglena gracilis
-
Manually annotated by BRENDA team
Nakazawa, M.; Takenaka, S.; Ueda, M.; Inui, H.; Nakano, Y.; Miyatake, K.
Pyruvate:NADP+ oxidoreductase is stabilized by its cofactor, thiamin pyrophosphate, in mitochondria of Euglena gracilis
Arch. Biochem. Biophys.
411
183-188
2003
Euglena gracilis
Manually annotated by BRENDA team