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Information on EC 1.2.1.42 - hexadecanal dehydrogenase (acylating) and Organism(s) Arabidopsis thaliana and UniProt Accession B9TSP7

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IUBMB Comments
Also acts, more slowly, on octadecanoyl-CoA.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: B9TSP7
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
fatty acid reductase, fatty acyl-coa reductase, atfar2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
fatty acyl-CoA reductase
-
dehydrogenase, hexadecanal (acylating)
-
-
-
-
fatty acid reductase
-
-
fatty acyl-CoA reductase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
hexadecanal:NAD+ oxidoreductase (CoA-acylating)
Also acts, more slowly, on octadecanoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
72561-01-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hexadecanoyl-CoA + NADPH + H+
hexadecanal + CoA + NADP+
show the reaction diagram
highest activity
-
-
?
octadecanoyl-CoA + NADPH + H+
octadecanal + CoA + NADP+
show the reaction diagram
-
-
-
?
tetradecanoyl-CoA + NADPH + H+
tetradecanal + CoA + NADP+
show the reaction diagram
-
-
-
?
heptadecanoyl-CoA + NADPH + H+
heptadecanal + CoA + NADP+
show the reaction diagram
-
-
-
-
?
hexadecanal + CoA + NAD+
hexadecanoyl-CoA + NADH + H+
show the reaction diagram
-
-
-
?
hexadecanoyl-CoA + NADPH + H+
hexadecanal + CoA + NADP+
show the reaction diagram
octadecanoyl-CoA + NADPH + H+
octadecanal + CoA + NADP+
show the reaction diagram
tetradecanoyl-CoA + NADPH + H+
tetradecanal + CoA + NADP+
show the reaction diagram
-
-
-
?
[hexadecanoyl]-acyl carrier protein + NADPH + H+
?
show the reaction diagram
-
preferred substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
heptadecanoyl-CoA + NADPH + H+
heptadecanal + CoA + NADP+
show the reaction diagram
-
-
-
-
?
hexadecanal + CoA + NAD+
hexadecanoyl-CoA + NADH + H+
show the reaction diagram
-
-
-
?
hexadecanoyl-CoA + NADPH + H+
hexadecanal + CoA + NADP+
show the reaction diagram
-
preferred substrate
-
-
?
octadecanoyl-CoA + NADPH + H+
octadecanal + CoA + NADP+
show the reaction diagram
-
low activity
-
-
?
[hexadecanoyl]-acyl carrier protein + NADPH + H+
?
show the reaction diagram
-
preferred substrate
-
-
?
additional information
?
-
-
no products are detected when using C18:1-CoA, 2-methyl-16:0-CoA, 2-methyl-18:0-CoA or C16:0 free fatty acid as substrates
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00531
hexadecanal
pH 7.0, 30°C, recombinant enzyme
0.0036
hexadecanoyl-CoA
-
in 100 mM phosphate buffer, pH 7.0, at 30°C
0.00146
[hexadecanoyl]-acyl carrier protein
-
in 100 mM phosphate buffer, pH 7.0, at 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
with aldehyde substrates
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme FAR6; ecotype Columbia
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
AtFAR2 is highly similar in properties and substrate specificity to AtFAR6
malfunction
mutations in specific genes encoding fatty acid reductases (FAR) re­sponsible for fatty alcohol production cause abnormal development of pollen. A disrupted AtFAR2 (MS2) gene in Arabidopsis thaliana results in pollen developing an abnormal exine layer and a reduced fertility pheno­type
physiological function
AtFAR2 is a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols, cf. EC 1.2.1.80. Enzyme AtFAR2 is the major enzyme respon­sible for exine layer functionality, AtFAR6 might provide functional redundancy to AtFAR2
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FACR6_ARATH
548
0
61626
Swiss-Prot
Secretory Pathway (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Ni2+ resin column chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Saccharomyces cerevisiae strain W303-1A and in Nicotiana benthamiana leaves
-
gene FAR2, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), transient expression of GFP-tagged FAR2 in Nicotiana benthamiana leaves under control of CaMV 35S promoter via Agrobacterium tumefaciens strain V3101::pMP90 transfection method
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Doan, T.T.; Carlsson, A.S.; Hamberg, M.; Buelow, L.; Stymne, S.; Olsson, P.
Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli
J. Plant Physiol.
166
787-796
2009
Arabidopsis thaliana (B9TSP7), Arabidopsis thaliana (Q08891), Arabidopsis thaliana (Q39152)
Manually annotated by BRENDA team
Doan, T.T.; Domergue, F.; Fournier, A.E.; Vishwanath, S.J.; Rowland, O.; Moreau, P.; Wood, C.C.; Carlsson, A.S.; Hamberg, M.; Hofvander, P.
Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana
Biochim. Biophys. Acta
1821
1244-1255
2012
Arabidopsis thaliana
Manually annotated by BRENDA team
Doan, T.T.; Carlsson, A.S.; Stymne, S.; Hofvander, P.
Biochemical characteristics of AtFAR2, a fatty acid reductase from Arabidopsis thaliana that reduces fatty acyl-CoA and -ACP substrates into fatty alcohols
Acta Biochim. Pol.
63
565-570
2016
Arabidopsis thaliana (Q08891), Arabidopsis thaliana
Manually annotated by BRENDA team