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Information on EC 1.2.1.38 - N-acetyl-gamma-glutamyl-phosphate reductase and Organism(s) Escherichia coli and UniProt Accession P11446

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Escherichia coli
UNIPROT: P11446 not found.
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
nagsd, n-acetyl-gamma-glutamyl-phosphate reductase, n-acetylglutamate 5-semialdehyde dehydrogenase, n-acetyl-gamma-glutamyl phosphate reductase, agpreductase, n-acetylglutamate 5-phosphate reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N-acetylglutamate 5-phosphate reductase
-
AGPR
-
-
-
-
AGPreductase
-
-
-
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N-acetyl-glutamate semialdehyde dehydrogenase
-
-
-
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N-acetyl-L-glutamate gamma-semialdehyde:NADP oxidoreductase (phosphorylating)
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-
-
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N-acetylglutamate 5-semialdehyde dehydrogenase
-
-
-
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N-acetylglutamic gamma-semialdehyde dehydrogenase
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-
-
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NAGSA dehydrogenase
-
-
-
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NAGSD
-
-
-
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reductase, acetyl-gamma-glutamyl phosphate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Phosphorylation
-
-
-
-
redox reaction
-
-
-
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oxidation
-
-
-
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reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acetyl-L-glutamate-5-semialdehyde:NADP+ 5-oxidoreductase (phosphorylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37251-00-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetyl-5-glutamyl phosphate + NADPH + H+
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
show the reaction diagram
substrate produced by AGK
-
-
?
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
N-acetyl-5-glutamyl phosphate + NADPH
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
N-acetyl-5-glutamyl phosphate + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
-
Fe2+
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slight
Mn2+
-
slight
N-ethylmaleimide
-
-
Ni2+
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slight
p-hydroxymercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
N-acetyl-L-glumate 5-semialdehyde
-
-
0.17
NADP+
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-
3
phosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 9.5
-
activity drops sharply below pH 9.0 and above 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
x * 38000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
-
4°C, 5 h
390280
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
gel filtration, enzyme unstable
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by His tag affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ligated into pET15b vector. Overexpressed as N-terminal His6 tag in Escherichia coli BL21 (DE3) cells carrying pLysS
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Baich, A.; Vogel, H.J.
N-acetyl-gamma-glutamokinase and N-acetylglutamic gamma-semialdehyde dehydrogenase: Repressible enzymes of arginine synthesis in Escherichia coli
Biochem. Biophys. Res. Commun.
7
491-496
1962
Escherichia coli, Escherichia coli Wc2
Manually annotated by BRENDA team
Glansdorff, N.; Sand, G.
Coordination of enzyme synthesis in the arginine pathway of Escherichia coli K-12
Biochim. Biophys. Acta
108
308-311
1965
Escherichia coli
Manually annotated by BRENDA team
Vogel, H.J.; McLellan, W.L.
N-Acetylglutamic gamma-semialdehyde dehydrogenase (Escherichia coli)
Methods Enzymol.
17A
255-260
1970
Escherichia coli
-
Manually annotated by BRENDA team
Takahara, K.; Akashi, K.; Yokota, A.
Continuous spectrophotometric assays for three regulatory enzymes of the arginine biosynthetic pathway
Anal. Biochem.
368
138-147
2007
Escherichia coli (P11446), Escherichia coli
Manually annotated by BRENDA team