Information on EC 1.2.1.3 - aldehyde dehydrogenase (NAD+) and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC1.2.1.3
Word Map on EC 1.2.1.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
This record set is specific for:
Homo sapiens


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea


The taxonomic range for the selected organisms is: Homo sapiens

EC NUMBER
COMMENTARY hide
1.2.1.3
-
RECOMMENDED NAME
GeneOntology No.
aldehyde dehydrogenase (NAD+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alkane oxidation
-
-
aromatic biogenic amine degradation (bacteria)
-
-
dimethylsulfoniopropanoate biosynthesis I (Wollastonia)
-
-
dimethylsulfoniopropanoate biosynthesis II (Spartina)
-
-
dopamine degradation
-
-
Entner-Doudoroff pathway III (semi-phosphorylative)
-
-
ethanol degradation II
-
-
ethanol degradation III
-
-
ethanol degradation IV
-
-
fatty acid alpha-oxidation I
-
-
fatty acid alpha-oxidation II
-
-
fatty acid alpha-oxidation III
-
-
histamine degradation
-
-
hypotaurine degradation
-
-
L-tryptophan degradation X (mammalian, via tryptamine)
-
-
NAD/NADP-NADH/NADPH mitochondrial interconversion (yeast)
-
-
noradrenaline and adrenaline degradation
-
-
octane oxidation
-
-
phytol degradation
-
-
putrescine degradation III
-
-
serotonin degradation
-
-
alanine metabolism
-
-
Entner Doudoroff pathway
-
-
histidine metabolism
-
-
non-pathway related
-
-
octane oxidation
-
-
propanol degradation
-
-
Glycolysis / Gluconeogenesis
-
-
Ascorbate and aldarate metabolism
-
-
Fatty acid degradation
-
-
Valine, leucine and isoleucine degradation
-
-
Lysine degradation
-
-
Arginine and proline metabolism
-
-
Histidine metabolism
-
-
Tryptophan metabolism
-
-
beta-Alanine metabolism
-
-
Glycerolipid metabolism
-
-
Pyruvate metabolism
-
-
Chloroalkane and chloroalkene degradation
-
-
Limonene and pinene degradation
-
-
Insect hormone biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Microbial metabolism in diverse environments
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
aldehyde:NAD+ oxidoreductase
Wide specificity, including oxidation of D-glucuronolactone to D-glucarate.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-86-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-N-(3-(6-(4-(1,4-dimethyl-3-oxopiperazin-2-yl)phenylamino)-4-methyl-5-oxo-4,5-dihydropyrazin-2-yl)-2-methylphenyl)-4,5,6,7-tetrahydrobenzo[b] thiophene-2-carboxamide + H2O + O2
?
show the reaction diagram
-
i.e. GDC-0834, a Bruton's tyrosine kinase inhibitor, a potential treatment of rheumatoid arthritis. GDC-0834
compound is extensively metabolized by amide hydrolysis by both aldehyde oxidase and carboxylesterase
-
?
1-formyl-6-methylpyrene + NAD+
6-methylpyrene 1-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-6-methylpyrene + NAD+ + H2O
6-methylpyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-8-methylpyrene + NAD+ + H2O
8-methylpyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-8-methylpyrene + NADP+
8-methylpyrene 1-carboxylate + NADPH + H+
show the reaction diagram
-
-
-
-
?
1-formylpyrene + NAD+
pyrene 1-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formylpyrene + NAD+ + H2O
pyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-naphthaldehyde + NAD+ + H2O
1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2,4-dinitrobenzaldehyde + NAD+ + H2O
2,4-dinitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
2-formylpyrene + NAD+
pyrene 2-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-formylpyrene + NAD+ + H2O
pyrene-2-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-hydroxy-3-nitrobenzaldehyde + NAD+ + H2O
2-hydroxy-3-nitrobenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-nonenal + NAD+ + H2O
(E)-non-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-quinolinone-4-carboxaldehyde + NAD+ + H2O
2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
3,4-dihydroxyphenylglycolaldehyde + NAD+ + H2O
3,4-dihydroxyphenylglycolate + NADH + H+
show the reaction diagram
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
3,4-dimethoxybenzoate + NADH
show the reaction diagram
-
-
-
-
?
3-pyridinecarboxaldehyde + NAD+ + H2O
3-pyridinecarboxylate + NADH
show the reaction diagram
-
-
-
-
?
4-aminobutyraldehyde + NAD+ + H2O
4-aminobutyrate + NADH
show the reaction diagram
-
-
-
-
?
4-dimethylamino-1-naphthaldehyde + NAD+ + H2O
4-dimethylamino-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-dimethylaminobenzaldehyde + NAD+ + H2O
4-dimethylaminobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NAD+
pyrene 4-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NAD+ + H2O
pyrene-4-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxy-2-nonenoate + NADH + H+
show the reaction diagram
-
low affinity for substrate binding and poor catalytic efficiency
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxynon-2-enal + NAD+
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
4-hydroxynonenal + NAD+ + H2O
4-hydroxynonaoate + NADH
show the reaction diagram
-
-
-
-
?
4-methoxy-1-naphthaldehyde + NAD+ + H2O
4-methoxy-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
4-methoxy-1-naphthaldehyde + NAD+ + H2O
4-methoxy-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-nitro-1-naphthaldehyde + NAD+ + H2O
4-nitro-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
4-oxonon-2-enal + NAD+ + H2O
4-oxonon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
5-bromo-1-naphthaldehyde + NAD+ + H2O
5-bromo-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
5-methoxyindole-3-carboxaldehyde + NAD+ + H2O
5-methoxyindole-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
6,7-dimethoxycoumarin-4-carboxaldehyde + NAD+ + H2O
6,7-dimethoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
6-(dimethylamino)-2-naphthaldehyde + NAD+ + H2O
6-(dimethylamino)-2-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
6-dimethylamino-2-naphthaldehyde + NAD+ + H2O
6-dimethylamino-2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
6-dimethylamino-2-naphthaldehyde + NAD+ + H2O
6-dimethylamino-2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthoate + NADH + H+
show the reaction diagram
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
6-methoxy-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
7-(dimethylamino)-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-(dimethylamino)coumarin-4-carboxaldehyde + NAD+ + H2O
7-(dimethylamino)coumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-acetoxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-acetoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-hydroxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-hydroxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-methoxy-1-naphthaldehyde + NAD+ + H2O
7-methoxy-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
7-methoxy-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
7-methoxy-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-methoxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-methoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
9-cis retinal + NAD+ + H2O
9-cis retinoic acid + NADH
show the reaction diagram
-
-
-
-
?
9-cis-retinal + NAD+ + H2O
9-cis-retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
acrolein + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
aldophosphamide + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
all-trans retinal + NAD+ + H2O
all-trans retinoic acid + NADH
show the reaction diagram
-
-
-
-
?
all-trans-retinal + NAD+ + H2O
all-trans-retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
alpha-aminoadipate semialdehyde + NAD+ + H2O
alpha-aminoadipate + NADH + H+
show the reaction diagram
-
-
-
?
alpha-aminoadipate semialdehyde + NAD+ + H2O
alpha-aminoadipic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
alpha-phenylpropanal + NAD+ + H2O
alpha-phenylpropionate + NADH
show the reaction diagram
-
-
-
-
?
an aldehyde + NAD+ + H2O
an acid + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
betaine aldehyde + NAD+ + H2O
betaine + NADH
show the reaction diagram
-
-
-
-
?
betaine aldehyde + NAD+ + H2O
betaine + NADH + H+
show the reaction diagram
-
-
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
cinnamic aldehyde + NAD+ + H2O
cinnamic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
dansyl-aminoacetaldehyde + ?
dansylglycine + ?
show the reaction diagram
-
the fluorescent substrate is used to monitor aldehyde dehydrogenase activity in fluorescence-activated cell sorting
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
fluorene-2-carboxaldehyde + NAD+ + H2O
fluorene-2-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+
formate + NADH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
activity with enzyme ALDH-2, no activity with enzyme ALDH-1
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
-
-
-
?
glutathione-4-oxonon-2-enal + NAD+ + H2O
glutathione-4-oxonon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
-
-
-
-
?
glyceryl trinitrate + NADH + H+
1,2-glyceryl dinitrate + nitrite + NAD+
show the reaction diagram
-
-
-
-
?
glyceryl trinitrate + NADH + H+
1,3-glyceryl dinitrate + nitrite + NAD+
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
heptanal + NAD+ + H2O
heptanoate + NADH
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
hydrocinnamaldehyde + NAD+ + H2O
hydrocinnamate + NADH
show the reaction diagram
-
-
-
-
?
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + NAD+ + H2O
indole-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
m-hydroxybenzaldehyde + NAD+ + H2O
m-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-methoxybenzaldehyde + NAD+ + H2O
m-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-methylbenzaldehyde + NAD+ + H2O
m-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
-
low affinity for substrate binding and poor catalytic efficiency
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
methylglyoxal + NAD+ + H2O
methylglyoxalate + NADH
show the reaction diagram
-
-
-
-
?
N-acetyl-4-aminobutyraldehyde + NAD+ + H2O
N-acetyl-4-aminobutyrate + NADH
show the reaction diagram
-
-
-
-
?
n-nonanal + NAD+ + H2O
n-nonanoate + NADH + H+
show the reaction diagram
nitroglycerin + NAD+ + H2O
1,2-glyceryl dinitrate + NO + NADH + H+
show the reaction diagram
-
-
-
-
?
o-hydroxybenzaldehyde + NAD+ + H2O
o-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
o-methoxybenzaldehyde + NAD+ + H2O
o-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
o-methylbenzaldehyde + NAD+ + H2O
o-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
o-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
-
high specificity
-
-
?
p-(dimethylamino)-benzaldehyde + NAD+ + H2O
p-(dimethylamino)benzoate + NADH
show the reaction diagram
-
-
-
-
?
p-(dimethylamino)cinnamaldehyde + NAD+ + H2O
p-(dimethylamino)cinnamate + NADH
show the reaction diagram
-
-
-
-
?
p-methoxybenzaldehyde + NAD+ + H2O
p-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
p-methylbenzaldehyde + NAD+ + H2O
p-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrocinnamaldehyde + NAD+ + H2O
p-nitrocinnamate + NADH
show the reaction diagram
-
-
-
-
?
pentanal + NAD+ + H2O
pentanoate + NADH
show the reaction diagram
-
-
-
-
?
phenanthrene-9-carboxaldehyde + NAD+ + H2O
phenanthrene-9-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + 1,N6-ethenoadenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + 3-acetylpyridine-adenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + 3-thionicotinamide-adenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + N-guanine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + N-hypoxanthine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + NAD+ + H2O
propanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propanoic acid + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
quinoline-3-carboxaldehyde + NAD+ + H2O
quinoline-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
quinoline-4-carboxaldehyde + NAD+ + H2O
quinoline-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
retinal + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
-
-
-
?
trans-cinnamaldehyde + NAD+ + H2O
trans-cinnamate + NADH
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
all-trans-retinal + NAD+ + H2O
all-trans-retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
alpha-aminoadipate semialdehyde + NAD+ + H2O
alpha-aminoadipate + NADH + H+
show the reaction diagram
P49419
-
-
-
?
an aldehyde + NAD+ + H2O
an acid + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
-
-
additional information
-
very low activity
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(4-chlorophenyl)-3-(1-piperidinyl)-1-propanone
-
-
1-ethoxycyclopropanol
-
-
2,4-dinitrobenzaldehyde
-
substrate inhibition
2,6-Dichloro-4-nitrophenol
-
primarily inhibits isoform ALDH2 (non-competitive), but does not affect isoform ALDH3A1
2-naphthaldehyde
-
substrate inhibition
3-(1-azepanyl)-1-phenyl-1-propanone
-
-
3-(dimethylamino)-1-(3-fluoro-4-methoxyphenyl)-1-propanone
-
-
3-(dimethylamino)-1-(4-ethylphenyl)-1-propanone
-
-
4-diethylaminobenzaldehyde
4-hydroxy-2-nonenal
-
4-hydroxy-2-nonenal completely inactivates ALDH2 activity in vitro at 100 mM
4-hydroxynon-2-enal
-
10% inhibition at 0.05 mM
4-oxonon-2-enal
-
90% inhibition at 0.05 mM
4-oxonon-2-enoic acid
-
90% inhibition at 0.05 mM
6-[O-(CH2)5-COOH]-2-naphthaldehyde
-
substrate inhibition
acetaldehyde
-
0.02 mM, substrate inhibition of isoenzyme I, no inhibition of isoenzyme II and III
acrolein
-
-
adenine
-
-
adenosine
-
-
adenosine 5'-monophosphate
-
competitive with NAD+ and noncompetitive with acetaldehyde
Alda-1
arsenite
-
47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4
Caffeine
-
-
Chloral hydrate
Chloroacetaldehyde
-
86% residual activity at 1 mM chloroacetaldehyde at pH 9.5
cyanamide
daidzin
-
-
diethylaminobenzaldehyde
-
0.025 mM diethylaminobenzaldehyde inhibits about 80% of the enzymatic activity
-
dimethyl ampal thiolester
-
78% inhibition of isozyme ALDH1 at 0.0075 mM
Disulfiram
glyceraldehyde
-
substrate inhibition
m-methylbenzaldehyde
-
substrate inhibition
methyl ampal thiolester
-
32% inhibition of isozyme ALDH1 at 0.0075 mM
methylglyoxal
-
substrate inhibition
molinate
-
irreversible inhibition of ALDH2
molinate sulfoxide
-
i.e. S-ethyl-hexahydro-1H-azepine-1-carbothioate sulfoxide, irreversible inhibition of ALDH2
N'-methylnicotinamide
-
-
NAD+
-
pronounced inhibition occurs at above 5 mM
nitroglycerin
o-nitrobenzaldehyde
-
substrate inhibition
p-methylbenzaldehyde
-
substrate inhibition
p-nitrobenzaldehyde
-
substrate inhibition
p-nitrocinnamaldehyde
-
substrate inhibition
theophylline
-
-
thio-NAD+
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
highest activity at 50 mM
Alda-1
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0358
1,N6-ethenoadenine dinucleotide
-
isoenzyme E3
0.00015 - 0.0016
1-formyl-6-methylpyrene
0.000027 - 0.004
1-formyl-8-methylpyrene
0.0009 - 0.004
1-formylpyrene
0.0000032
2,4-dinitrobenzaldehyde
-
enzyme ALDH-2
0.00038 - 0.011
2-formylpyrene
0.000008 - 0.00046
2-naphthaldehyde
0.00024
2-naphthalene carboxylate
-
apparent Km value, diphosphate buffer, pH 8.1, cofactor NADH
0.0053
2-nonenal
-
pH and temperature not specified in the publication
0.0005 - 0.003
3,4-dihydroxyphenyl acetaldehyde
0.0004 - 0.0042
3,4-dihydroxyphenylacetaldehyde
0.00033
3,4-dimethoxybenzaldehyde
-
enzyme ALDH-2
0.00196
3-Pyridinecarboxaldehyde
-
enzyme ALDH-2
0.005 - 0.014
4-aminobutyrate
0.0042
4-Dimethylaminobenzaldehyde
-
recombinant ALDH3A1
0.00004 - 0.00078
4-formylpyrene
3.383
4-hydroxy-2-nonenal
-
at 25C, pH not specified in the publication
0.155
4-hydroxy-3-methoxybenzaldehyde
-
recombinant ALDH3A1
0.0179
4-hydroxynonenal
-
pH 8.0, 25C
0.000065
4-methoxy-1-naphthaldehyde
-
enzyme ALDH-2
0.019
4-methoxybenzaldehyde
-
recombinant ALDH3A1
0.0002
4-methyoxy-1-naphthaldehyde
-
enzyme ALDH-1
0.0000063
5-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-1
0.0000004 - 0.0000025
5-bromo-1-naphthaldehyde
0.0189
5-methoxyindole-3-carboxaldehyde
-
enzyme ALDH-2
0.0000004 - 0.000011
5-nitro-1-naphthaldehyde
0.00069
6,7-dimethoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.0000023
6-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-2
0.0017 - 0.02
6-dimethylamino-2-naphthaldehyde
0.00016 - 0.0002
6-methoxy-2-naphthaldehyde
0.0054
6-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00213
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.000062
7-(dimethylamino)-coumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00006
7-acetoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.15
7-hydroxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.05
7-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00028
7-methoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00005 - 80
acetaldehyde
0.017
acetylaldehyde
-
at substrate concentrations 0-0.07 mM
0.009
acrolein
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.0093
all-trans-retinal
-
at pH 8.0 and 25C
0.169
alpha-aminoadipate semialdehyde
-
pH and temperature not specified in the publication
0.00093
alpha-phenylpropanal
-
enzyme ALDH-2
0.000018 - 0.5302
benzaldehyde
0.0411 - 0.26
Betaine aldehyde
0.0234
betaNAD+
-
isoenzyme E3
0.00055
cinnamaldehyde
-
-
0.006
cinnamic aldehyde
-
recombinant ALDH3A1
0.0000029 - 0.000022
decanal
0.000054
fluorene-2-carboxaldehyde
-
enzyme ALDH-1
0.32 - 0.42
formaldehyde
0.183
glutathione-4-oxonon-2-enal
-
-
0.205 - 7.374
glyceraldehyde
0.046
glycoaldehyde
-
isoenzyme 2, pH 7.4
0.005 - 0.24
glycolaldehyde
0.000018 - 0.000027
Heptanal
0.00003 - 0.0391
Hexanal
0.0005
hydrocinnamaldehyde
-
enzyme ALDH-2
0.0007 - 0.001
indole 3-acetaldehyde
0.00015 - 0.00031
Indole-3-acetaldehyde
0.01 - 0.02
Indole-3-aldehyde
-
enzyme ALDH-2
0.00009
m-methoxybenzaldehyde
-
enzyme ALDH-2
0.000018
m-methylbenzaldehyde
-
enzyme ALDH-2
0.0043 - 0.466
malondialdehyde
0.0086 - 1.876
methylglyoxal
0.11
N-acetyl-4-aminobutyrate
-
enzyme from liver
0.944
N-guanine dinucleotide
-
isoenzyme E3
0.172
N-hypoxanthine dinucleotide
-
isoenzyme E3
0.0008 - 0.0285
n-nonanal
0.0003 - 16
NAD+
0.0008
o-methoxybenzaldehyde
-
enzyme ALDH-2
1.3
o-methylbenzaldehyde
-
enzyme ALDH-2
0.0000063
o-nitrobenzaldehyde
-
enzyme ALDH-2
0.000012 - 0.0175
octanal
0.00002
p-(dimethylamino)-benzaldehyde
-
enzyme ALDH-2
0.00006
p-(dimethylamino)benzaldehyde
-
enzyme ALDH-1
0.000005 - 0.00142
p-(dimethylamino)cinnamaldehyde
0.000018
p-Methoxybenzaldehyde
-
enzyme ALDH-2
0.000017
p-methylbenzaldehyde
-
enzyme ALDH-2
0.000007
p-nitrobenzaldehyde
-
enzyme ALDH-2
0.0000007
p-nitrocinnamaldehyde
-
enzyme ALDH-2
0.000034 - 0.00016
pentanal
0.000004
phenanthrene-9-carboxaldehyde
-
enzyme ALDH-2
0.000029 - 0.0055
phenylacetaldehyde
0.000008 - 1
propanal
0.0028 - 12
propionaldehyde
0.00033
quinoline-3-carboxaldehyde
-
enzyme ALDH-2
0.0028
quinoline-4-carboxaldehyde
-
enzyme ALDH-2
0.000035 - 0.0004
trans-cinnamaldehyde
additional information
additional information
-
Km-values of the 475 and 264 arginine mutants of Oriental variant E847K
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.667
2,4-dinitrobenzaldehyde
-
enzyme ALDH-2
4
2-naphthaldehyde
-
enzyme ALDH-2
1.57
2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
1.42
3,4-dimethoxybenzaldehyde
-
enzyme ALDH-2
27.7
3-Pyridinecarboxaldehyde
-
enzyme ALDH-2
4.42
4-methoxy-1-naphthaldehyde
-
enzyme ALDH-1
2.67
5-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-1
0.158 - 0.25
5-bromo-1-naphthaldehyde
0.55 - 0.833
5-nitro-1-naphthaldehyde
0.983
6,7-dimethoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.617
6-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-2
0.983
6-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
2.17
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
1.3
7-(dimethylamino)-coumarin-3-carboxaldehyde
-
enzyme ALDH-2
7.9
7-(dimethylamino)coumarin-4-carboxaldehyde
-
enzyme ALDH-2
1.08
7-acetoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
8.33
7-hydroxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
5.33
7-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
3.17
7-methoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
13.2 - 19.7
acetaldehyde
15.2
alpha-phenylpropanal
-
enzyme ALDH-2
5.83
benzaldehyde
-
enzyme ALDH-2
3.83 - 11.7
decanal
2.83 - 6
fluorene-2-carboxaldehyde
67.5
formaldehyde
-
enzyme ALDH-2
0.04
glutathione-4-oxonon-2-enal
-
50 mM sodium phosphate buffer pH 7.4, 1 mM NAD+, 37C
4.33 - 22.7
Heptanal
4.17 - 28.5
Hexanal
12.3
hydrocinnamaldehyde
-
enzyme ALDH-2
9.33 - 11.3
Indole-3-acetaldehyde
1
m-hydroxybenzaldehyde
-
enzyme ALDH-2
5.83
m-methoxybenzaldehyde
-
enzyme ALDH-2
4.5
m-methylbenzaldehyde
-
enzyme ALDH-2
0.0667 - 3.5
NAD+
0.367
o-methoxybenzaldehyde
-
enzyme ALDH-2
2.75
o-methylbenzaldehyde
-
enzyme ALDH-2
1.23
o-nitrobenzaldehyde
-
enzyme ALDH-2
4.17 - 15
octanal
2.33
p-(dimethylamino)-benzaldehyde
-
enzyme ALDH-2
2.13
p-(dimethylamino)benzaldehyde
-
enzyme ALDH-1
1.5 - 8.17
p-(dimethylamino)cinnamaldehyde
2.5
p-Methoxybenzaldehyde
-
enzyme ALDH-2
3
p-methylbenzaldehyde
-
enzyme ALDH-2
7.17
p-nitrobenzaldehyde
-
enzyme ALDH-2
0.45
p-nitrocinnamaldehyde
-
enzyme ALDH-2
8.17 - 22.8
pentanal
0.3
phenanthrene-9-carboxaldehyde
-
enzyme ALDH-2
30 - 50
phenylacetaldehyde
0.42 - 19.7
propanal
11.2
quinoline-3-carboxaldehyde
-
enzyme ALDH-2
4.67
quinoline-4-carboxaldehyde
-
enzyme ALDH-2
2.5 - 7.83
trans-cinnamaldehyde
additional information
additional information
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027 - 0.117
NAD+
0.517 - 3.13
propanal
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0074
2,6-Dichloro-4-nitrophenol
-
isoform ALDH2, in 0.1 M sodium diphosphate buffer (pH 9.5), at 21-23C
3.5
Caffeine
-
apparent Ki value
0.17
Chloral hydrate
-
-
0.0002
Disulfiram
-
isoenzyme 1
2 - 12
methylglyoxal
0.053 - 0.133
NADH
7.3
theophylline
-
apparent Ki value
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0068 - 0.0083
1-(4-chlorophenyl)-3-(1-piperidinyl)-1-propanone
0.0022 - 0.0086
3-(1-azepanyl)-1-phenyl-1-propanone
0.0025 - 0.0064
3-(dimethylamino)-1-(3-fluoro-4-methoxyphenyl)-1-propanone
0.0054 - 0.0079
3-(dimethylamino)-1-(4-ethylphenyl)-1-propanone
2.5
Caffeine
Homo sapiens
-
-
3.5
theophylline
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000047
-
tested group, n = 32, squamous cell cancer, mean
0.000048
-
tested group, n = 59, healthy tissue, mean
0.000049
-
tested group, n = 59, esophageal cancer, mean
0.00005
-
non-drinkers, n = 24, healthy tissue, mean
0.000051
-
drinkers, n = 35, healthy tissue, mean; non-drinkers, n = 24, healthy tissue, median
0.000052
-
non-drinkers, n = 24, esophageal cancer, mean; tested group, n = 27, adenocarcinoma, mean; tested group, n = 59, healthy tissue, median
0.000053
-
tested group, n = 32, squamous cell cancer, median
0.000054
-
drinkers, n = 35, esophageal cancer, mean; drinkers, n = 35, healthy tissue, median; non-drinkers, n = 24, esophageal cancer, median
0.000055
-
tested group, n = 59, esophageal cancer, median
0.000058
-
drinkers, n = 35, esophageal cancer, median
0.000059
-
tested group, n = 27, adenocarcinoma, median
0.000119
-
total group, n = 44, healthy tissue, mean
0.00012
-
non-drinkers, n = 25, healthy tissue, mean
0.000123
-
drinkers, n = 19, healthy tissue, mean; non-drinkers, n = 25, healthy tissue, median
0.000125
-
total group, n = 44, healthy tissue, median
0.000128
-
drinkers, n = 19, healthy tissue, median
0.000179
-
metastatic tumor, mean
0.00018
-
conversion of glycerol trinitrate to 1,3-glycerol dinitrate
0.000185
-
primary tumor, mean
0.000186
-
metastatic tumor, median
0.000188
-
non-drinkers, n = 25, cancer tissue, mean
0.00019
-
non-drinkers, n = 25, cancer tissue, median; total group, n = 44, cancer tissue, mean
0.000192
-
primary tumor, median
0.000193
-
total group, n = 44, cancer tissue, median
0.000194
-
drinkers, n = 19, cancer tissue, mean
0.000198
-
drinkers, n = 19, cancer tissue, median
0.00066
-
conversion of glycerol trinitrate to 1,3-glycerol dinitrate
0.00088
-
-
0.003
-
conversion of glycerol trinitrate to 1,2-glycerol dinitrate
0.009
-
conversion of glycerol trinitrate to 1,2-glycerol dinitrate
0.137
-
-
0.2341
-
substrate: propionaldehyde, pH 8.0, 25C
0.29
-
isoenzyme I
0.375
-
recombinant enzyme, using propionaldehyde as the substrate, in 50 mM sodium phosphate buffer, pH 7.4, at 37C
0.445
-
isoenzyme III
0.462
-
-
0.5
-
isoenzyme ALDH1 from atypical liver
0.51
-
ALDH1 from usual human liver
0.54
-
isoenzyme E1
0.58
-
isoenzyme 1
0.79
-
ALDH2 from usual human liver
1.01
-
isoenzyme E2
1.3
-
isoenzyme E2
3
-
isoenzyme II
additional information
-
men, n = 34, control, age 56 - 70, median 2.953 mU/l, range 1.721 - 6.227 mU/l; men, n = 34, gastric cancer, age 58 - 72, median 3.002 mU/l, range 1.734 - 7.038 mU/l; total group, n = 55, control, age 53 - 70, median 2.918 mU/l, range 1.635 - 6.227 mU/l; total group, n = 55, gastric cancer, age 54 - 72, median 2.983 mU/l, range 1.624 - 7.038 mU/l; women, n = 21, control, age 53 - 67, median 2.906 mU/l, range 1.635 - 5.224 mU/l; women, n = 21, gastric cancer, age 54 - 70, median 2.968 mU/l, range 1.624 - 6.163 mU/l
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
activity assay
8
-
reaction with indole 3-acetaldehyde, isoenzyme III
8.8
-
activity assay
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
pH 7.0: about 66% of maximal activity, pH 9.0: about 85% of maximal activity, isoenzyme 1
8 - 9.5
-
pH 8.0: about 35% of maximal activity, above pH 9.0: optimum, isoenzyme 2
8.5 - 10.5
-
pH 8.5: about 40% of the activity of isoenzyme ALDH1 and 2, pH 10.5: about 50% of the activity of isoenzyme ALDH2, about 65% of the activity of isoenzyme ALDH1
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
activity assay
37
-
incubation of ALDH with 14C-labeled nitroglycerin
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
-
about
6.3
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
aneurysm wall
Manually annotated by BRENDA team
-
increased activity in colorectal cancer cells
Manually annotated by BRENDA team
-
tissue with highest content
Manually annotated by BRENDA team
-
ALDH1A1 is preferentially expressed in the CD133+ subpopulation
Manually annotated by BRENDA team
-
aldehyde dehydrogenase activity correlates with the viability of cells taken from peripheral blood stem cell grafts
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50390
-
calculated from sequence of cDNA
51500
-
4 * 51500, SDS-PAGE
52000
-
determined by SDS-PAGE
52600
-
4 * 52600, isoenzyme ALDH1, SDS-PAGE; 4 * 52600, isoenzyme ALDH2, SDS-PAGE
53000
-
x * 53000, enzyme form ALDH-2, SDS-PAGE
54200
-
4 * 54200, isoenzyme 2, SDS-PAGE
54400
-
4 * 54400
54800
-
4 * 54800, isoenzyme 1, SDS-PAGE
55450
-
MALDI-TOF mass spectrometry
56000
-
SDS-PAGE
57000
-
determined by SDS-PAGE and Western Blot analysis
58000
-
x * 58000, enzyme form ALDH-1, SDS-PAGE
85000
-
2 * 85000, GFP-tagged enzyme, SDS-PAGE
170000
-
GFP-tagged enzyme, gel filtration
208000 - 210000
-
isoenzyme I, non-denaturing PAGE
210000
-
gel filtration
216000
220000
-
isoenzyme III, gel filtration
224000
-
gel filtration
225000
230000
-
isoenzyme E2
236000
-
isoenzyme III, non-denaturing PAGE
245000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homotetramer
tetramer