Information on EC 1.2.1.28 - benzaldehyde dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.2.1.28
-
RECOMMENDED NAME
GeneOntology No.
benzaldehyde dehydrogenase (NAD+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
benzaldehyde + NAD+ + H2O = benzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-chlorotoluene degradation II
-
-
benzoate biosynthesis II (CoA-independent, non-beta-oxidative)
-
-
benzoate biosynthesis III (CoA-dependent, non-beta-oxidative)
-
-
m-xylene degradation to m-toluate
-
-
mandelate degradation I
-
-
p-xylene degradation to p-toluate
-
-
salicin biosynthesis
-
-
salicortin biosynthesis
-
-
toluene degradation to benzoate
-
-
Xylene degradation
-
-
Toluene degradation
-
-
Aminobenzoate degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
benzaldehyde:NAD+ oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37250-93-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
; MTCC2445
-
-
Manually annotated by BRENDA team
sp. DJ77
-
-
Manually annotated by BRENDA team
sp. DJ77
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-bromobenzaldehyde + NAD+ + H2O
2-bromobenzoate + NADH
show the reaction diagram
-
-
-
?
2-chlorobenzaldehyde + NAD+ + H2O
2-chlorobenzoate + NADH + H+
show the reaction diagram
2-fluorobenzaldehyde + NAD+ + H2O
2-fluorobenzoate + NADH + H+
show the reaction diagram
-
41% of activity with benzaldehyde
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
2-hydroxybenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
2-hydroxybenzoate + NADH + H+
show the reaction diagram
2-methoxybenzaldehyde + NAD+ + H2O
2-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
2-methylbenzoate + NADH
show the reaction diagram
2-methylbenzaldehyde + NAD+ + H2O
2-methylbenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
2-methylbenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
naphthalene-2-carboxylic acid + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
3,4-dihydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH
show the reaction diagram
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
3-chlorobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-fluorobenzaldehyde + NAD+ + H2O
3-fluorobenzoate + NADH + H+
show the reaction diagram
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
3-hydroxybenzoate + NADH + H+
show the reaction diagram
3-methoxy-4-hydroxybenzaldehyde + NAD+ + H2O
3-methoxy-4-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH
show the reaction diagram
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
3-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH
show the reaction diagram
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
3-methylbenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH
show the reaction diagram
4-carboxybenzaldehyde + NAD+ + H2O
4-carboxybenzoate + NADH
show the reaction diagram
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH
show the reaction diagram
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-ethylbenzaldehyde + NAD+ + H2O
4-ethylbenzoate + NADH
show the reaction diagram
-
-
-
?
4-fluorobenzaldehyde + NAD+ + H2O
4-fluorobenzoate + NADH + H+
show the reaction diagram
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
show the reaction diagram
4-methoxy-3-hydroxybenzaldehyde + NAD+ + H2O
4-methoxy-3-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + H+
show the reaction diagram
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH
show the reaction diagram
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
4-methylbenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + 2 H+
show the reaction diagram
-
-
-
-
ir
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
benzaldehyde + NADP+ + H2O
benzoate + NADPH
show the reaction diagram
-
best substrate
-
-
?
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + H+
show the reaction diagram
-
benzaldehyde dehydrogenase II
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
-
-
-
?
furan-2-carboxyaldehyde + NAD+ + H2O
furan-2-formiate + NADH
show the reaction diagram
-
benzaldehyde dehydrogenase II
-
?
hexan-1-al + NAD+ + H2O
hexanoate + NADH
show the reaction diagram
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-anisaldehyde + NAD+ + H2O
m-anisate + NADH + H+
show the reaction diagram
-
-
-
-
?
octan-1-al + NAD+ + H2O
octanoate + NADH
show the reaction diagram
perillaldehyde + NAD+ + H2O
perillic acid + NADH
show the reaction diagram
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
pyridin-3-carboxyaldehyde + NAD+ + H2O
pyridin-3-formiate + NADH
show the reaction diagram
-
benzaldehyde dehydrogenase II
-
?
pyridine 4-carboxyaldehyde + NAD+ + H2O
pyridine 4-formiate + NADH
show the reaction diagram
salicylaldehyde + NAD+ + H2O
salicylate + NADH + H+
show the reaction diagram
-
-
-
-
?
thiophen-2-carboxyaldehyde + NAD+ + H2O
thiophen-2-formiate + NADH
show the reaction diagram
-
benzaldehyde dehydrogenase II
-
?
trans-cinnamaldehyde + NAD+ + H2O
trans-cinnamate + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
-
not active with NADP+
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cs+
-
benzaldehyde dehydrogenase I
Li+
-
benzaldehyde dehydrogenase I
Mg2+
-
5.1 mol Mg2+ per mol enzyme; tightly bound
Na+
-
benzaldehyde dehydrogenase I
NH4+
-
benzaldehyde dehydrogenase I
Rb+
-
benzaldehyde dehydrogenase I
additional information
-
BZDH shows the presence of 5.1 mol of magnesium per mole of the enzyme; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, and Zn2+ up to 2.5 mM in the assay mixture do not affect activity; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, or Zn2+ (up to 2.5 mM) does not affect the enzymatic activity; metal chelators at 10 mM like EDTA and EGTA do not affect activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
2,2'-dipyridyl
-
40% residual activity at 1 mM; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
2-Bromobenzaldehyde
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0.1 mM, 90% inhibition, benzaldehyde dehydrogenase II
2-Chlorobenzaldehyde
-
0.1 mM, 100% inhibition, benzaldehydedehydrogenase II
2-Fluorobenzaldehyde
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0.1 mM, 100% inhibition, benzaldehydedehydrogenase II
2-Hydroxybenzaldehyde
-
substrate inhibition
2-Methylbenzaldehyde
-
substrate inhibition
2-naphthaldehyde
-
substrate inhibition
3-Chlorobenzaldehyde
-
substrate inhibition
3-Hydroxybenzaldehyde
-
substrate inhibition
3-Methoxybenzaldehyde
-
substrate inhibition
3-methylbenzaldehyde
4-Chlorobenzaldehyde
-
substrate inhibition
4-chloromercuribenzoate
4-Isopropylbenzaldehyde
-
0.01 mM, 87% inhibition, benzaldehyde dehydrogenase II
4-methoxybenzaldehyde
-
substrate inhibition
4-methylbenzaldehyde
-
substrate inhibition
benzaldehyde
Disulfiram
-
-
iodoacetamide
iodoacetate
N-ethylmaleimide
pentafluorobenzaldehyde
-
0.1 mM, 44% inhibition, benzaldehyde dehydrogenase II
additional information
-
metal chelators (10 mM) like EDTA and EGTA do not affect the activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chitosan
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.187 - 0.497
2-Hydroxybenzaldehyde
0.026 - 0.377
2-Methylbenzaldehyde
0.0038
2-naphthaldehyde
-
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
0.0021 - 0.0028
3-Chlorobenzaldehyde
0.0029 - 0.0039
3-Fluorobenzaldehyde
0.0028 - 0.063
3-Hydroxybenzaldehyde
0.0026 - 0.129
3-Methoxybenzaldehyde
0.00038 - 0.0019
3-methylbenzaldehyde
0.022
3-Nitrobenzaldehyde
-
-
0.003
4-Chlorobenzaldehyde
-
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
0.0028
4-Fluorobenzaldehyde
-
-
0.018
4-hydroxybenzaldehyde
-
-
0.0035 - 0.016
4-methoxybenzaldehyde
0.00097 - 0.02
4-methylbenzaldehyde
0.00201
acetaldehyde
-
-
0.00057 - 0.46
benzaldehyde
0.0015
cinnamaldehyde
-
-
0.059
furan-2-carboxyaldehyde
-
-
0.072
hexan-1-al
-
-
0.067 - 0.33
NAD+
0.46
octan-1-al
-
-
0.152
perillaldehyde
-
-
0.00535
phenylacetaldehyde
-
-
0.026
Pyridine-3-carboxaldehyde
-
-
0.02
pyridine-4-carboxaldehyde
-
-
0.0051
thiophen-2-carboxaldehyde
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
33 - 41
2-Hydroxybenzaldehyde
39
2-Methylbenzaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
56
2-naphthaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
16
3-Chlorobenzaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
41
3-Fluorobenzaldehyde
-
-
24 - 29
3-Hydroxybenzaldehyde
33 - 885
3-Methoxybenzaldehyde
23
3-methylbenzaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
67
4-Chlorobenzaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
64
4-Fluorobenzaldehyde
-
-
20
4-hydroxybenzaldehyde
-
-
49 - 101
4-methoxybenzaldehyde
120
4-methylbenzaldehyde
-
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
2.08
acetaldehyde
-
-
0.31 - 72
benzaldehyde
26
cinnamaldehyde
-
-
46
furan-2-carboxaldehyde
-
-
17
perillaldehyde
-
-
0.23
phenylacetaldehyde
-
-
68
Pyridine-3-carboxaldehyde
-
-
66
pyridine-4-carboxaldehyde
-
-
52
thiophen-2-carboxyaldehyde
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
60
2-Hydroxybenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
110
2-Methylbenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
14700
2-naphthaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
5700
3-Chlorobenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
9000
3-Hydroxybenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
6800
3-Methoxybenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
13000
3-methylbenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
22000
4-Chlorobenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
29000
4-methoxybenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
6000
4-methylbenzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
10000
benzaldehyde
-
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.808
2-Hydroxybenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.521
2-Methylbenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.008
2-naphthaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.068
3-Chlorobenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.131
3-Hydroxybenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.0013
3-Methoxybenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.051
3-methylbenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.013
4-Chlorobenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.035
4-methoxybenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.003
4-methylbenzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication
0.006
benzaldehyde
-
apparent Ki-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0006
-
basal level of enzyme activity
0.002
-
benzaldehyde dehydrogenase II and class 1 mutant activity in cell extracts of wild-type grown without inducer
0.0022
-
maximum activity in 5-day-old cell cultures 17 h after treatment with chitosan, 25 mg/l
0.006
-
class 1 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol or 1 mM benzaldehyde
0.094
-
class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde
0.126
-
benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde
0.32
-
cell free extract, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
0.324
-
benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol
0.325
-
class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol
0.433
-
benzylbenzoate-grown cells
64
-
after 200fold purification, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
additional information
-
substrate 3-hydroxybenzaldehyde, relative activity 11%, benzaldehyde 100%; substrate acetaldehyde, relative activity 309%, benzaldehyde 100%; substrate formaldehyde, relative activity 22%, benzaldehyde 100%; substrate hexanal, relative activity 50%, benzaldehyde 100%; substrate m-anisaldehyde, relative activity 25%, benzaldehyde 100%; substrate p-anisaldehyde, relative activity 31%, benzaldehyde 100%; substrate phenylacetaldehyde, relative activity 62%, benzaldehyde 100%; substrate propionaldehyde, relative activity 165%, benzaldehyde 100%; substrate salicylaldehyde, relative activity 9%, benzaldehyde 100%; substrate trans-cinnamaldehyde, relative activity 47%, benzaldehyde 100%
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
activity assay
9 - 9.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29
-
activity assay
40
-
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
5-days-old, without or after treatment with methyl jasmonate for 6 h
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51654
-
4 * 51654, dehydrogenase II, deduced from nucleotide sequence, electrospray MS
51941
-
4 * 51941, deduced from nucleotide sequence
52500
-
4 * 52500, SDS-PAGE
54400
-
4 * 54400, nucleotide sequence, SDS-PAGE
54500
-
monomer
55000
-
4 * 55000, dehydrogenase II, SDS-PAGE
56000
-
4 * 56000, dehydrogenase I, SDS-PAGE
56300
-
4 * 56300, SDS-PAGE
57000
-
subunit molecular mass, determined by SDS–PAGE
117000
-
gel filtration; native molecular mass of BZDH, determined by Sephacryl S-300-HR gel filtration chromatography
122000 - 126000
-
gel filtration
141000 - 219000
-
dehydrogenase I, gel filtration with different gels
180000
-
strain N.C.I.B. 8250, gel filtration
208000
-
gel filtration
225000
-
dehydrogenase II, gel filtration
232400
-
tetramer, determined by gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homotetramer
-
4 * 54500
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithiothreitol stabilizes
the enzyme loses its activity irreversibly when dialyzed against 50 mM potassium phosphate, pH 6.5, 5% (v/v) glycerol, 2 mM dithiothreitol without benzyl alcohol for 5 h. Enzyme activity is unaffected by varying the concentration of benzyl alcohol
-
the presence of magnesium may be essential for the stability of the enzyme
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 100 mM potassium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 1 mM benzamidine, 1.2 mM phenylmethanesulfonylfluoride, 40% glycerol, 3 months, 10% loss of activity
-
purified enzyme preparations are found to be stable and stored either at 4 or -20°C, stable up to 6 months at -20°C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration; to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography
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by affinity chromatography on Ni-NTA agarose
-
dehydrogenases I and II
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soluble proteins are extracted from elicitor-treated cell cultures
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli DH10B
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into the vector pET-28a+ for expression in Escherichia coli BL21 Rosetta cells, into the pEF1-LIS binary vector for transformation of petunia flowers with Agrobacterium tumefaciens, for subcellular localization experiments in Arabidopsis protoplasts GFP fusion proteins are constructed using the vector p326-SGFP
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
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in cells of Acinetobacter sp. AG1 isolated from the River Elbe a combined action of benzylalcohol and benzaldehyde dehydrogenase induced after growth with benzylbenzoate does produce benzoate from benzylalcohol, which is a mechanism to quantitatively eliminate the anthropogenic marker compound benzylbenzoate under aerobic conditions
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