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Information on EC 1.2.1.26 - 2,5-dioxovalerate dehydrogenase and Organism(s) Azospirillum brasilense and UniProt Accession Q08IC0

for references in articles please use BRENDA:EC1.2.1.26
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This record set is specific for:
Azospirillum brasilense
UNIPROT: Q08IC0 not found.
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The taxonomic range for the selected organisms is: Azospirillum brasilense
Synonyms
2-oxoglutarate semialdehyde dehydrogenase, alpha-ketoglutarate semialdehyde dehydrogenase, alpha-ketoglutaric semialdehyde dehydrogenase, alpha-KGSA dehydrogenase, alphaKGSA dehydrogenase, alphaKGSADH, DopDH, KGSADH, KGSADH-I, KGSADH-II, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-oxoglutarate semialdehyde dehydrogenase
-
-
-
-
alpha-ketoglutaric semialdehyde dehydrogenase
alphaKGSA dehydrogenase
280439, 285510, 285511
-
KGSADH-I
280439
-
KGSADH-II
285510
-
KGSADH-III
285511
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2,5-dioxopentanoate:NADP+ 5-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37250-92-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
show the reaction diagram
-
-
-
?
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
prefered cofactor of KGSADH-II
NADP+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-galactarate
induces KGSADH-II
D-glucarate
induces KGSADH-II
hydroxy-L-proline
induces KGSADH-III
L-arabinose
induces KGSADH-I
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.0246
alpha-Ketoglutaric semialdehyde
0.0065 - 0.0075
alpha-Ketoglutaric semialdehyde
0.2599 - 1.136
NAD+
0.0149 - 2.427
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.4 - 53.9
alpha-Ketoglutaric semialdehyde
21.3 - 23
alpha-Ketoglutaric semialdehyde
16.7 - 52.3
NAD+
9.86 - 15.3
NADP+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC29145
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
KGSD2_AZOBR
525
0
54351
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54351
2 * 57000 or 2 * 54351, gel filtration
57000
2 * 57000 or 2 * 54351, gel filtration
55185
2 * 57000 or 2 * 55185, gel filtration
57000
2 * 57000 or 2 * 55185, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 57000 or 2 * 54351, gel filtration
dimer
2 * 57000 or 2 * 55185, gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-35°C, buffer E, pH 8.0, 250 mM imidazole
-35°C, buffer E, pH 8.0, 250 mM imidazole
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Watanabe, S.; Yamada, M.; Ohtsu, I.; Makino, K.
alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution
J. Biol. Chem.
282
6685-6695
2007
Azospirillum brasilense, Azospirillum brasilense (Q08IB7), Azospirillum brasilense (Q08IC0), Azospirillum brasilense (Q1JUP4), Azospirillum brasilense ATCC 29145 (Q08IB7), Azospirillum brasilense ATCC 29145 (Q08IC0), Azospirillum brasilense ATCC 29145 (Q1JUP4), Bacillus subtilis
Manually annotated by BRENDA team
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