Information on EC 1.18.1.4 - rubredoxin-NAD(P)+ reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.18.1.4
-
RECOMMENDED NAME
GeneOntology No.
rubredoxin-NAD(P)+ reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 reduced rubredoxin + NAD(P)+ + H+ = 2 oxidized rubredoxin + NAD(P)H
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
non-pathway related
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Fatty acid degradation
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-
SYSTEMATIC NAME
IUBMB Comments
rubredoxin:NAD(P)+ oxidoreductase
The enzyme from Pyrococcus furiosus requires FAD. It reduces a number of electron carriers, including benzyl viologen, menadione and 2,6-dichloroindophenol, but rubredoxin is the most efficient. Ferredoxin is not utilized.
CAS REGISTRY NUMBER
COMMENTARY hide
114514-31-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme is possibly a part of a defense mechanism against oxygen toxicity
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
cytochrome c from horse heart
-
-
?
2 oxidized rubredoxin + NADH
2 reduced rubredoxin + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 oxidized rubredoxin + NADPH
2 reduced rubredoxin + NADP+ + H+
show the reaction diagram
2,6-dichloroindophenol + NADH
reduced 2,6-dichloroindophenol + NAD+ + H+
show the reaction diagram
-
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADH
?
show the reaction diagram
-
-
-
-
?
benzyl viologen + NADH
reduced benzyl viologen + NAD+
show the reaction diagram
-
-
-
-
?
FAD + NADH
FADH2 + NAD+
show the reaction diagram
-
-
-
-
?
Fe(III) citrate + NADH
Fe(II) citrate + NAD+
show the reaction diagram
-
-
-
-
?
FMN + NADH
FMNH2 + NAD+
show the reaction diagram
-
-
-
-
?
methyl viologen + NADH
reduced methyl viologen + NAD+ + H+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADH
reduced benzyl viologen + NAD+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADH
reduced benzyl viologen + NAD+ + H+
show the reaction diagram
-
-
-
?
oxidized benzyl viologen + NADPH
reduced benzyl viologen + NADP+
show the reaction diagram
-
-
-
-
?
oxidized benzyl viologen + NADPH
reduced benzyl viologen + NADP+ + H+
show the reaction diagram
-
-
-
?
oxidized Clostridium pasteurianum rubredoxin + NADH
reduced Clostridium pasteurianum rubredoxin + NAD+ + H+
show the reaction diagram
oxidized Clostridium pasteurianum rubredoxin + NADPH
reduced Clostridium pasteurianum rubredoxin + NADP+ + H+
show the reaction diagram
oxidized flavodiiron protein + NADH
reduced flavodiiron protein + NAD+ + H+
show the reaction diagram
oxidized flavodiiron protein + NADPH
reduced flavodiiron protein + NADP+ + H+
show the reaction diagram
oxidized Pyrococcus furiosus rubredoxin + NADH
reduced Pyrococcus furiosus rubredoxin + NAD+ + H+
show the reaction diagram
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-
-
-
?
oxidized Pyrococcus furiosus rubredoxin + NADPH
reduced Pyrococcus furiosus rubredoxin + NADP+ + H+
show the reaction diagram
-
-
-
-
?
oxidized rubredoxin + NADH
reduced rubredoxin + NAD+
show the reaction diagram
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rubredoxin is the best substrate
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-
?
oxidized rubredoxin + NADPH
reduced rubredoxin + NADP+
show the reaction diagram
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-
-
-
?
oxidized rubrerythrin + NADH
reduced rubrerythrin + NAD+ + H+
show the reaction diagram
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-
-
-
?
oxidized rubrerythrin + NADPH
reduced rubrerythrin + NADP+ + H+
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 oxidized rubredoxin + NADH
2 reduced rubredoxin + NAD+ + H+
show the reaction diagram
-
-
-
-
?
2 oxidized rubredoxin + NADPH
2 reduced rubredoxin + NADP+ + H+
show the reaction diagram
oxidized rubredoxin + NADPH
reduced rubredoxin + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metals can be detected
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no substrate inhibition at high NAD(P)H (up to 0.3 mM)
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
the enzyme contains one flavin adenine dinucleotide (FAD) molecule per mol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034 - 0.18
NADH
0.005 - 0.048
NADPH
5.5
Oxidized benzyl viologen
pH 8, 80°C, cosubstrate: NADH
0.0028 - 0.0043
oxidized Clostridium pasteurianum rubredoxin
-
0.0032 - 0.0052
oxidized Pyrococcus furiosus rubredoxin
-
0.0016 - 0.05
oxidized rubredoxin
-
0.0095 - 0.05
rubredoxin
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
354 - 498
NADH
258 - 341
NADPH
1236
Oxidized benzyl viologen
pH 8, 80°C, cosubstrate: NADH
3 - 13.9
oxidized Clostridium pasteurianum rubredoxin
-
9.6 - 69.4
oxidized Pyrococcus furiosus rubredoxin
-
221 - 15000
oxidized rubredoxin
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2770 - 10400
NADH
5375 - 68300
NADPH
225
Oxidized benzyl viologen
pH 8, 80°C, cosubstrate: NADH
5000 - 8400
oxidized Clostridium pasteurianum rubredoxin
-
3000 - 13000
oxidized Pyrococcus furiosus rubredoxin
-
22050 - 300000
oxidized rubredoxin
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
pH 8.0, 80°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 80
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about 70fold increase in kcat/Km for oxidized rebredoxin increases from 25°C to 80°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2
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calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39900
x * 39900, calculated from sequence
40000
x * 40000, SDS-PAGE
41000
-
gel filtration
51000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 39900, calculated from sequence; x * 40000, SDS-PAGE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
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12 h, 50% loss of activity
95
-
2 h, 50% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HiTrap IMAC-Ni2+ column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
when the organism is grown on maltose plus S(0) the specific activity of the enzyme is highest under this growth condition, slightly lower on the maltose-only medium, and lower still in the three peptide-based media
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