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Information on EC 1.18.1.2 - ferredoxin-NADP+ reductase and Organism(s) Spinacia oleracea and UniProt Accession P00455

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IUBMB Comments
A flavoprotein (FAD). In chloroplasts and cyanobacteria the enzyme acts on plant-type [2Fe-2S] ferredoxins, but in other bacteria it can also reduce bacterial [4Fe-4S] ferredoxins and flavodoxin.
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This record set is specific for:
Spinacia oleracea
UNIPROT: P00455
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Word Map
The taxonomic range for the selected organisms is: Spinacia oleracea
The enzyme appears in selected viruses and cellular organisms
Synonyms
nitrite reductase, adrenodoxin reductase, ferric reductase, ferredoxin-nadp+ reductase, ferredoxin-nadp reductase, ferredoxin-nadp(+) reductase, ferredoxin-nadp+ oxidoreductase, flavodoxin reductase, ferredoxin:nadp+ oxidoreductase, ferredoxin:nadp+ reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adrenodoxin reductase
-
-
-
-
cytochrome b6f -associated ferredoxin:NADP+ oxidoreductase
-
-
DA1
-
-
-
-
ferredoxin (flavodoxin)-NAD(P)H reductase
-
-
ferredoxin (flavodoxin)-NADP(H) reductase
-
-
ferredoxin-NAD(P)H reductase
-
-
ferredoxin-NADP oxidoreductase
-
-
-
-
ferredoxin-NADP reductase
-
-
-
-
Ferredoxin-NADP(+) reductase
-
-
-
-
ferredoxin-NADP(H) oxidoreductase
-
-
ferredoxin-NADP(H) reductase
-
-
ferredoxin-NADP+ reductase
-
-
ferredoxin-NADP-oxidoreductase
-
-
-
-
ferredoxin-nicotinamide-adenine dinucleotide phosphate (oxidized) reductase
-
-
-
-
ferredoxin-TPN reductase
-
-
-
-
ferredoxin: NADP(+) oxidoreductase
-
-
ferredoxin:NADP(+) oxidoreductase
-
-
ferredoxin:NADP+ oxidoreductase
Flavodoxin reductase
-
-
-
-
FLDR
-
-
-
-
FLXR
-
-
-
-
NADP:ferredoxin oxidoreductase
-
-
-
-
NADPH:ferredoxin oxidoreductase
-
-
-
-
NFR
-
-
-
-
reduced nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase
-
-
-
-
reductase, ferredoxin-nicotinamide adenine dinucleotide phosphate
-
-
-
-
TPNH-ferredoxin reductase
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ferredoxin:NADP+ oxidoreductase
A flavoprotein (FAD). In chloroplasts and cyanobacteria the enzyme acts on plant-type [2Fe-2S] ferredoxins, but in other bacteria it can also reduce bacterial [4Fe-4S] ferredoxins and flavodoxin.
CAS REGISTRY NUMBER
COMMENTARY hide
56367-57-8
-
9029-33-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NAD(P)H
2 ferrocyanide + NAD(P)+ + H+
show the reaction diagram
-
diaphorase activity
-
-
?
2 ferricyanide + NADPH
2 ferrocyanide + NADP+ + H+
show the reaction diagram
2 reduced ferredoxin + NADP+
2 oxidized ferredoxin + NADPH + H+
show the reaction diagram
-
-
-
-
r
2 reduced ferricyanide + NADP+
2 oxidized ferricyanide + NADPH
show the reaction diagram
-
-
-
-
r
aclacinomycin A + NADP+
7-deoxyaklavinone + NADPH
show the reaction diagram
-
under anaerobic conditions
-
-
?
daunomycin + NADP+
7-deoxydaunomycinone + NADPH
show the reaction diagram
-
under anaerobic conditions
-
-
?
dibromothymoquinone + NADPH
? + NADP+
show the reaction diagram
-
diaphorase activity, no direct involvement of thiol or amino groups in the reaction
-
-
?
dibromothymoquinone + NADPH
reduced dibromothymoquinone + NADP+
show the reaction diagram
K3Fe(CN)6 + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
menogarol + NADP+
7-deoxynogarol + NADPH
show the reaction diagram
-
under anaerobic conditions
-
-
?
NADPH + acceptor
NADP+ + reduced acceptor
show the reaction diagram
-
the diaphorase reaction with NADPH and different electron acceptors, such as ferricyanide, complexed transition metals, substituted phenols, nitro derivatives, tetrazolium salts, NAD+, viologens, quinones, and cytochromes, is mostly irreversible, probably due to restrictions of formation of the caged radical pair and/or the covalent (C4alpha)-flavin hydroperoxide intermediates required for efficient oxygen reduction, acceptors enhance the oxidation reaction several fold, e.g. ferredoxin, flavodoxin, viologens, nitro derivatives, and quinones, that can readily engage in oxygen-dependent redox cycling leading to formation of superoxide
-
-
?
NADPH + oxidized ferredoxin
NADP+ + reduced ferredoxin
show the reaction diagram
nogalamycin + NADP+
7-deoxynogalarol + NADPH
show the reaction diagram
-
under anaerobic conditions
-
-
?
oxidized ferredoxin I + NADH + H+
reduced ferredoxin I + NAD+
show the reaction diagram
-
-
-
-
?
oxidized rubredoxin + NADP+
reduced rubredoxin + NADPH
show the reaction diagram
-
Clostridium pasteurianum rubredoxin
-
-
?
plastoquinone + NADPH
? + NADP+
show the reaction diagram
-
plastoquinones with different length of side chains from spinach, preference for short chain substrate, reaction proceeds via a FMN and a semiquinone intermediate, incorporation of the substrate into sodium cholate micelles is required for activity, micelles structure scheme
-
-
?
reduced ferredoxin + NAD+
oxidized ferredoxin + NADH + H+
show the reaction diagram
-
-
-
-
r
reduced ferredoxin + NADP+
oxidized ferredoxin + NADPH
show the reaction diagram
reduced ferredoxin + NADP+
oxidized ferredoxin + NADPH + H+
show the reaction diagram
-
-
-
-
r
reduced ferredoxin I + NADP+
oxidized ferredoxin I + NADPH
show the reaction diagram
-
-
-
-
r
reduced ferredoxin I + NADP+
oxidized ferredoxin I + NADPH + H+
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 reduced ferredoxin + NADP+
2 oxidized ferredoxin + NADPH + H+
show the reaction diagram
-
-
-
-
r
reduced ferredoxin + NADP+
oxidized ferredoxin + NADPH
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ferredoxin
-
NAD(P)H
-
NADPH is absolutely preferred over NADH by the wild-type isozymes
NAD+
-
enzyme reduces NADP+ and NAD+, specific for NADP+ reduction under physiological conditions
NADP+
NADPH
additional information
-
poor activity with NAD(H)
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
stimulation at 0.1 M
NH4+
-
activator of ferredoxin-NADP+ reductase
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',5'-ADP
-
competitive inhibition, but there could also be a non-competitive component caused by binding at a weak secondary NADP+ binding site
2',5'-ATP-ribose
-
competitive inhibition, competitive in forming complexes with reductase
diphosphate
-
inhibitor of ferredoxin-dependent photoreduction
disulfodisalicylidenepropane-1,1-diamine
-
inhibits all reactions except photoreduction of cytochrome c
Ferredoxin
-
heparin
-
binds to the enzyme, inhibits ferredoxin and NADPH binding to the enzyme
iodoacetamide
-
-
Mercurials
-
-
-
Mg2+
-
inhibits reduction of plastoquinone incorporated into sodium cholate micelles
myristyltrimethylammonium bromide
-
inhibits reduction of plastoquinone almost completely at 15 mM
N-ethyl-3(3-dimethylaminopropyl)carbodiimide
-
-
NADPH
-
reversible inhibition, is turned to irreversible in presence of 4 M urea
octylglucoside
-
inhibits reduction of plastoquinone
oxidized ferredoxin
-
inhibits binding of reduced ferredoxin and reduction of flavin
SDS
-
inhibits reduction of plastoquinone almost completely at 15 mM
Triazine dyes
-
Triton X-100
-
inhibits reduction of plastoquinone
Zn2+
-
80% inhibition
[Cr[CN]6]4-
-
binds to the enzyme
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ferredoxin
-
highly stimulates NADPH oxidation
-
NADP+
-
stimulates binding of reduced ferredoxin and reduction of flavin
polylysine
-
activator of ferredoxin-NADP+ reductase
sodium cholate
-
stimulated NADPH oxidation, especially in presence of plastoquinone substrate
additional information
-
acceptors enhance the oxidation reaction several fold, e.g. ferredoxin, flavodoxin, viologens, nitro derivatives, and quinones, that can readily engage in oxygen-dependent redox cycling leading to formation of superoxide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0119 - 0.0132
dibromothymoquinone
0.049 - 0.053
Ferredoxin
-
0.097 - 0.1
K3Fe(CN)6
-
-
3.77
NAD+
-
-
4.2
NADH
-
pH 7.0, 15°C, recombinant chimeric enzyme
0.00722
NADP+
-
-
0.0031 - 0.043
NADPH
0.0025 - 0.0027
reduced ferredoxin I
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.6 - 25
dibromothymoquinone
9.6
NADH
-
pH 7.0, 15°C, recombinant chimeric enzyme, diaphorase activity
12.5 - 560
NADPH
200 - 600
reduced ferredoxin
45 - 115
reduced ferredoxin I
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.72
Cd2+
-
pH 8.7, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
76
-
diaphorase activity of the 32 kDa enzyme
79
-
diaphorase activity of the 35 kDa enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
8.7
-
assay at
9
-
diaphorase activity decreases at lower pH
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
assay at
additional information
-
enzyme thermal inactivation is irreversible
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
weakly bound
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme associated with the cytochrome b6f complex can participate in the cyclic electron transport in the chloroplast as photosystem I-plastoquinone or NADPH-plastoquinone oxidoreductase. Ferredoxin is not directly required for plastoquinone reduction but is rather necessary for the reduction of ferredoxin reductase under light conditions during operation of the cyclic electron transport
physiological function
additional information
-
the correct distribution of FNR between stroma and thylakoids is used to efficiently regulate ferredoxin-dependent electron partitioning in the chloroplast
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FENR_SPIOL
369
0
41188
Swiss-Prot
Chloroplast (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
117000
-
gel filtration, P-1
31000
-
truncated form after limited proteolysis with proteases, deletion of N-terminal region
32000
-
lack of the first 28 amino acid residues after expression in Escherichia coli
33000
-
SDS-PAGE
33000 - 36000
-
ultracentrifugation
33500 - 42000
-
multiple forms: a, b, c, d, e, SDS-PAGE, disc gel electrophoresis
34000
-
SDS-PAGE, monomer
35000
36000
-
SDS-PAGE
37000
50000
-
gel filtration, P-2
70000
-
SDS-PAGE, dimer
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 35000, SDS-PAGE
monomer
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallizations of oxidized enzyme, dithionite-reduced enzyme, 2',5'-ADP-complexed enzyme, enzyme mutants S96V, E312A, E312L, and E312Q, crystal structure determinations and analysis at 1.7-2.0 A resolution, overview
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S96G
-
shows 2% of wild type activity
S96V
-
shows only 0.05% of wild type activity
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
recombinant chimeric enzyme is slightly less stable than the wild-type parent isozymes
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 2 months in nitrogen or in air
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from spinach leaves in several steps, to homogeneity
-
purification method development for cytochrome b6f -associated ferredoxin:NADP+ oxidoreductase, overview
recombinant chimeric enzyme, comprising the root NADP-binding domain and the leaf FAD-binding domain, from Escherichia coli
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
functional expression of chimeric enzyme, comprising the root NADP-binding domain and the leaf FAD-binding domain, in Escherichia coli
-
two forms, one lacks the first 28 amino acid residues, has full diaphorase activity but reduced NADPH/cytochrome-c activity
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shin, M.; Tagawa, K.; Arnon, D.I.
Crystallization of ferredoxin-TPN reductase and its role in the photosynthetic apparatus of chloroplasts
J. Biochem.
338
84-96
1963
Spinacia oleracea
Manually annotated by BRENDA team
Aliverti, A.; Jansen, T.; Zanetti, G.; Ronchi, S.; Herrmann, R.G.; Curti, B.
Expression in Escherichia coli of ferredoxin:NADP+ reductase from spinach. Bacterial synthesis of the holoflavoprotein and of an active enzyme form lacking the first 28 amino acid residues of the sequence
Eur. J. Biochem.
191
551-555
1990
Spinacia oleracea
Manually annotated by BRENDA team
Armstrong, F.A.; Corbett, S.G.
Inhibition of ferredoxin: NADP+ reductase activity by the hexacyanochromate (III) ion
Biochem. Biophys. Res. Commun.
141
578-583
1986
Spinacia oleracea
Manually annotated by BRENDA team
Matthijs, H.C.P.; Coughlan, S.J.; Hind, G.
Removal of ferredoxin:NADP+ oxidoreductase from thylakoid membranes, rebinding to depleted membranes, and identification of the binding site
J. Biol. Chem.
261
12154-12158
1986
Spinacia oleracea
Manually annotated by BRENDA team
Hosler, J.P.; Yocum, C.F.
Heparin inhibition of ferredoxin-NADP reductase in chloroplast thylakoid membranes
Arch. Biochem. Biophys.
236
473-478
1985
Spinacia oleracea
Manually annotated by BRENDA team
Carrillo, N.; Lucero, H.A.; Vallejos, R.H.
Light modulation of chloroplast membrane-bound ferredoxin-NADP+ oxidoreductase
J. Biol. Chem.
256
1058-1059
1981
Spinacia oleracea
Manually annotated by BRENDA team
Sherriff, S.; Teller, D.C.; Herriott, J.R.
Ferredoxin-NADP+ oxidoreductase is active as a monomer with molecular weight 33000-36000
Arch. Biochem. Biophys.
205
499-502
1980
Spinacia oleracea
Manually annotated by BRENDA team
Zanetti, G.; Arosio, P.
Solubilization from spinach thylakoids of a higher molecular weight form of ferredoxin-NADP+ reductase
FEBS Lett.
111
373-376
1980
Spinacia oleracea
Manually annotated by BRENDA team
Ellefson, W.L.; Krogmann, D.W.
Studies of the multiple forms of ferredoxin-NADP oxidoreductase from spinach
Arch. Biochem. Biophys.
194
593-599
1979
Spinacia oleracea
Manually annotated by BRENDA team
Bookjans, G.; San Pietro, A.; Bger, P.
Resolution and reconstitution of spinach ferredoxin-NADP+ reductase
Biochem. Biophys. Res. Commun.
80
759-765
1978
Spinacia oleracea, Spinacia oleracea Atlanta
Manually annotated by BRENDA team
Gozzer, C.; Zanetti, G.; Galliano, M.; Sacchi, G.A.; Minchiotti, L.; Curti, B.
Molecular heterogeneity of ferredoxin-NADP+ reductase from spinach leaves
Biochim. Biophys. Acta
485
278-290
1977
Spinacia oleracea
Manually annotated by BRENDA team
Shahak, Y.; Crowther, D.; Hind, G.
The involvement of ferredoxin-NADP+ reductase in cyclic electron transport in chloroplasts
Biochim. Biophys. Acta
636
234-243
1981
Spinacia oleracea
Manually annotated by BRENDA team
Bhme, H.
On the role of ferredoxin and ferredoxin-NADP+ reductase in cyclic electron transport of spinach chloroplasts
Eur. J. Biochem.
72
283-289
1977
Spinacia oleracea
Manually annotated by BRENDA team
Fredricks, W.W.; Gehl, J.M.
Multiple forms of ferredoxin-nicotinamide adenine dinucleotide phosphate reductase from spinach
Arch. Biochem. Biophys.
174
666-674
1976
Spinacia oleracea
Manually annotated by BRENDA team
Levy, L.M.; Bets, G.F.
Interaction of NADPH and triazine dyes with ferredoxin-NADP+ oxidoreductase
Biochim. Biophys. Acta
955
236-242
1988
Spinacia oleracea
Manually annotated by BRENDA team
Zanetti, G.; Morelli, D.; Ronchi, S.; Negri, A.; Aliverti, A.; Curti, B.
Structural studies on the interaction between ferredoxin and ferredoxin-NADP+ reductase
Biochemistry
27
3753-3759
1988
Spinacia oleracea
-
Manually annotated by BRENDA team
Apley, E.C.; Wagner, R.; Engelbrecht, S.
Rapid procedure for the preparation of ferredoxin-NADP+ oxidoreductase in molecularly pure form at 36 kDa
Anal. Biochem.
150
145-154
1985
Spinacia oleracea
Manually annotated by BRENDA team
Batie, C.J.; Kamin, H.
Electron transfer by ferredoxin:NADP+ reductase. Rapid-reaction evidence for participation of a ternary complex
J. Biol. Chem.
259
11976-11985
1984
Spinacia oleracea
Manually annotated by BRENDA team
Wada, K.; Tamura, T.; Matsubara, H.; Kodo, K.
Spirulina ferredoxin-NADP+ reductase. Further characterization with an improved preparation
J. Biochem.
94
387-393
1983
Spinacia oleracea, Spirulina sp.
Manually annotated by BRENDA team
Carrillo, N.; Vallejos, R.H.
Interaction of ferredoxin-NADP+ oxidoreductase with triazine dyes. A rapid purification method by affinity chromatography
Biochim. Biophys. Acta
742
285-294
1983
Spinacia oleracea
Manually annotated by BRENDA team
Zanetti, G.
The reduction of iodonitrotetrazolium chloride by ferredoxin-NADP+ reductase: a new tool for the characterization of the spinach chloroplast flavoprotein
Plant Sci. Lett.
23
55-61
1981
Spinacia oleracea
-
Manually annotated by BRENDA team
Zanetti, G.; Curti, B.
Ferredoxin-NADP+ oxidoreductase
Methods Enzymol.
69
250-255
1980
Spinacia oleracea
-
Manually annotated by BRENDA team
Suss, K.H.
Isolation and partial characterization of membrane-bound ferredoxin-NADP+-reductase from chloroplasts
FEBS Lett.
101
305-310
1979
Spinacia oleracea
Manually annotated by BRENDA team
Shin, M.
Ferredoxin-NADP reductase from spinach
Methods Enzymol.
23
440-447
1971
Spinacia oleracea
-
Manually annotated by BRENDA team
Batie, C.J.; Kamin, H.
The relation of pH and oxidation-reduction potential to the association state of the ferredoxin-ferredoxin:NADP+ reductase complex
J. Biol. Chem.
256
7756-7763
1981
Spinacia oleracea
Manually annotated by BRENDA team
Sherriff, S.; Heriott, J.R.
Structure of ferrdoxin-NADP+ oxidoreductase and the location of the NADP binding site
J. Mol. Biol.
145
441-451
1981
Spinacia oleracea
Manually annotated by BRENDA team
Bhme H.
Quantitative determination of ferredoxin, ferredoxin-NADP+ reductase and plastocyanin in spinach chloroplasts
Eur. J. Biochem.
83
137-141
1978
Spinacia oleracea, Spinacia oleracea Atlanta
Manually annotated by BRENDA team
Zanetti, G.; Aliverti, A.; Curti, B.
A cross-linked complex eetween ferredoxin and ferredoxin-NADP+ reductase
J. Biol. Chem.
259
6153-6157
1984
Spinacia oleracea
Manually annotated by BRENDA team
Przysiecki, C.T.; Bhattacharyya, A.K.; Tollin, G.; Cusanovich, M.A.
Kinetics of reduction of clostridium pasteurianum rubredoxin by laser photoreduced spinach ferredoxin:NADP+ reductase and free flavins
J. Biol. Chem.
260
1452-1458
1985
Spinacia oleracea
Manually annotated by BRENDA team
Fisher, J.; Abdella, B.R.J.; McLane, K.E.
Anthracycline antibiotic reduction by spinach ferredoxin-NADP+ reductase and ferredoxin
Biochemistry
24
3562-3571
1985
Spinacia oleracea
Manually annotated by BRENDA team
Clark, R.D.; Hawkesford M.J.; Coughlan, J.B.; Hind, G.
Association of ferredoxin-NADP+ oxidoreductase with the chloroplast cytochrome b-f complex
FEBS Lett.
174
137-142
1984
Spinacia oleracea
-
Manually annotated by BRENDA team
Gadda, G.; Aliverti, A.; Ronchi, S.; Zanetti, G.
Structure-function relationship in spinach ferredoxin-NADP+ reductase as studied by limited proteolysis
J. Biol. Chem.
265
11955-11959
1990
Spinacia oleracea
Manually annotated by BRENDA team
Batie, C.J.; Kamin, H.
Ferredoxin-NADP+ reductase
J. Biol. Chem.
259
8832-8839
1984
Spinacia oleracea
Manually annotated by BRENDA team
Berzborn, R.J.; Klein-Hitpa, L.; Otto, J.; Schunemann, S.; Oworah-Nkruma, R.; Meyer, H.E.
The "additional" CF0II of the photosysthetic ATP-synthase and the thylakoid polypeptide, binding ferredoxin-NADP+ reductase: are they different?
Z. Naturforsch. C
45
772-784
1990
Spinacia oleracea
Manually annotated by BRENDA team
Batie, C.J.; Kamin, H.
Association of ferredoxin-NADP+ reductase with NADP(H) specificity and oxidation-reduction properties
J. Biol. Chem.
261
11214-11223
1986
Spinacia oleracea
Manually annotated by BRENDA team
Shin, M.; Tsujita, M.; Tomizawa, H.; Sakihama, N.; Kamei, K.; Oshino R.
Proteolytic degradation of ferredoxin-NADP+ reductase during purification from spinach
Arch. Biochem. Biophys.
279
97-103
1990
Spinacia oleracea
Manually annotated by BRENDA team
Shin M.
Complex formation by ferredoxin-NADP+ reductase with ferredoxin or NADP+
Biochim. Biophys. Acta
292
13-19
1973
Spinacia oleracea
Manually annotated by BRENDA team
Aliverti, A.; Faber, R.; Finnerty, C.M.; Ferioli, C.; Pandini, V.; Negri, A.; Karplus, P.A.; Zanetti, G.
Biochemical and crystallographic characterization of ferredoxin-NADP+ reductase from nonphotosynthetic tissues
Biochemistry
40
14501-14508
2001
Zea mays, Spinacia oleracea (P00455), Spinacia oleracea
Manually annotated by BRENDA team
Bruns C.M.; Karplus P.A.
Refined crystal structure of spinach ferredoxin reductase at 1.7A resolution: oxidized, reduced and 2'-phospho-5'AMP bound states
J. Mol. Biol.
247
125-145
1995
Spinacia oleracea
Manually annotated by BRENDA team
Aliverti, A.; Bruns, C.M.; Pandini, V.E.; Karplus P.A.; Vanoni, M.A.; Curti, B.; Zanitti, G.
Involvement of serine 96 in the catalytic mechanism of ferredoxin-NADP+-reductase: structure-function relationship as studies by site-directed mutagenesis and x-ray crystallography
Biochemistry
34
8371-8379
1995
Spinacia oleracea
Manually annotated by BRENDA team
Aliverti, A.; Pandini, V.; Zanetti, G.
Domain exchange between isoforms of ferredoxin-NADP+ reductase produces a functional enzyme
Biochim. Biophys. Acta
1696
93-101
2004
Spinacia oleracea
Manually annotated by BRENDA team
Ceccarelli, E.A.; Arakaki, A.K.; Cortez, N.; Carrillo, N.
Functional plasticity and catalytic efficiency in plant and bacterial ferredoxin-NADP(H) reductases
Biochim. Biophys. Acta
1698
155-165
2004
Azotobacter vinelandii, Anabaena sp., Chlamydomonas reinhardtii, Cyanophora paradoxa, Escherichia coli, Leptospira interrogans, Rhodobacter capsulatus, Spinacia oleracea, Anabaena sp. PCC7119
Manually annotated by BRENDA team
Carrillo, N.; Ceccarelli, E.A.
Open questions in ferredoxin-NADP+ reductase catalytic mechanism
Eur. J. Biochem.
270
1900-1915
2003
Azotobacter vinelandii, Anabaena sp., Capsicum annuum, Cyanobacteria, Escherichia coli, Pisum sativum, Rhodobacter capsulatus, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Grzyb, J.; Waloszek, A.; Latowski, D.; Wieckowski, S.
Effect of cadmium on ferredoxin:NADP+ oxidoreductase activity
J. Inorg. Biochem.
98
1338-1346
2004
Spinacia oleracea
Manually annotated by BRENDA team
Medina, M.; Gomez-Moreno, C.
Interaction of ferredoxin-NADP+ reductase with its substrates: optimal interaction for efficient electron transfer
Photosynth. Res.
79
113-131
2004
Azotobacter vinelandii, Anabaena sp., Escherichia coli, Spinacia oleracea
Manually annotated by BRENDA team
Karplus, P.A.; Faber, H.R.
Structural aspects of plant ferredoxin: NADP(+) oxidoreductases
Photosynth. Res.
81
303-315
2004
Trichormus variabilis, Capsicum annuum, Pisum sativum, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Grzyb, J.; Bojko, M.; Wieckowski, S.
Effects of amino and thiol group reagents on the ferredoxin:NADP+ oxidoreductase catalysed reduction of dibromothymoquinone
Photosynthetica
41
627-630
2003
Spinacia oleracea
-
Manually annotated by BRENDA team
Bojko, M.; Kruk, J.; Wieckowski, S.
Plastoquinones are effectively reduced by ferredoxin:NADP+ oxidoreductase in the presence of sodium cholate micelles. Significance for cyclic electron transport and chlororespiration
Phytochemistry
64
1055-1060
2003
Spinacia oleracea
Manually annotated by BRENDA team
Grzyb, J.; Malec, P.; Rumak, I.; Garstka, M.; Strzalka, K.
Two isoforms of ferredoxin:NADP(+) oxidoreductase from wheat leaves: purification and initial biochemical characterization
Photosynth. Res.
96
99-112
2008
Spinacia oleracea, Triticum aestivum (Q8RVZ8), Triticum aestivum (Q8RVZ9), Triticum aestivum
Manually annotated by BRENDA team
Szymanska, R.; Dluzewska, J.; Slesak, I.; Kruk, J.
Ferredoxin:NADP+ oxidoreductase bound to cytochrome b6f complex is active in plastoquinone reduction: implications for cyclic electron transport
Physiol. Plant.
141
289-298
2010
Spinacia oleracea, Spinacia oleracea (P00455)
Manually annotated by BRENDA team
Grzyb, J.; Bojko, M.; Waloszek, A.; Strzalka, K.
Ferredoxin:NADP+ oxidoreductase as a target of Cd2+ inhibitory action - biochemical studies
Phytochemistry
72
14-20
2011
Spinacia oleracea, Triticum aestivum
Manually annotated by BRENDA team
Benz, J.P.; Lintala, M.; Soll, J.; Mulo, P.; Boelter, B.
A new concept for ferredoxin-NADP(H) oxidoreductase binding to plant thylakoids
Trends Plant Sci.
15
608-613
2010
Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team