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Information on EC 1.18.1.1 - rubredoxin-NAD+ reductase and Organism(s) Alcanivorax borkumensis and UniProt Accession Q0VTB0

for references in articles please use BRENDA:EC1.18.1.1
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IUBMB Comments
Requires FAD. The enzyme from Clostridium acetobutylicum reduces rubredoxin, ferricyanide and dichlorophenolindophenol, but not ferredoxin or flavodoxin. The reaction does not occur when NADPH is substituted for NADH. Contains iron at the redox centre.
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This record set is specific for:
Alcanivorax borkumensis
UNIPROT: Q0VTB0
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Word Map
The taxonomic range for the selected organisms is: Alcanivorax borkumensis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
reduced rubredoxin
+
+
=
oxidized rubredoxin
+
Synonyms
rubredoxin reductase, nadh:rubredoxin oxidoreductase, nadh rubredoxin oxidoreductase, nadh-rubredoxin oxidoreductase, (flavo)rubredoxin reductase, nad(p)h:rubredoxin reductase, reduced nicotinamide adenine dinucleotide-rubredoxin reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ABO_0162
locus name
(flavo)rubredoxin reductase
-
-
-
-
dihydronicotinamide adenine dinucleotide-rubredoxin reductase
-
-
-
-
DPNH-rubredoxin reductase
-
-
-
-
FIRd-reductase
-
-
-
-
NADH-rubredoxin oxidoreductase
-
-
-
-
NADH-rubredoxin reductase
-
-
-
-
NADH: rubredoxin oxidoreductase
-
-
-
-
NOR
-
-
-
-
reduced nicotinamide adenine dinucleotide-rubredoxin reductase
-
-
-
-
reductase, rubredoxin-nicotinamide adenine dinucleotide
-
-
-
-
rubredoxin reductase
-
-
-
-
rubredoxin-NAD reductase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
rubredoxin:NAD+ oxidoreductase
Requires FAD. The enzyme from Clostridium acetobutylicum reduces rubredoxin, ferricyanide and dichlorophenolindophenol, but not ferredoxin or flavodoxin. The reaction does not occur when NADPH is substituted for NADH. Contains iron at the redox centre.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-27-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NADH + H+ + oxidized methyl thiazolyl tetrazolium
NAD+ + reduced methyl thiazolyl tetrazolium
show the reaction diagram
-
-
-
?
NADH + H+ + oxidized rubredoxin-2
NAD+ + reduced rubredoxin
show the reaction diagram
i.e. AlkG. Isoform RubB can reduce AlkG, therefore compensating for the absence of AlkT, also a rubredoxin reductase, missing inAlcanivorax borkumensis SK2 genome
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6
NADH
pH 8.0, 45°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11500
pH 8.0, 45°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
x * 41000, calculated and SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 41000, calculated and SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Teimoori, A.; Ahmadian, S.; Madadkar-Sobhani, A.; Bambai, B.
Rubredoxin reductase from Alcanivorax borkumensis: expression and characterization
Biotechnol. Prog.
27
1383-1389
2011
Alcanivorax borkumensis (Q0VTB0), Alcanivorax borkumensis, Alcanivorax borkumensis SK2 (Q0VTB0)
Manually annotated by BRENDA team