Information on EC 1.17.7.2 - 7-hydroxymethyl chlorophyll a reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.17.7.2
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RECOMMENDED NAME
GeneOntology No.
7-hydroxymethyl chlorophyll a reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chlorophyll a + H2O + 2 oxidized ferredoxin = 71-hydroxychlorophyll a + 2 reduced ferredoxin + 2 H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chlorophyll cycle
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chlorophyll metabolism
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Porphyrin and chlorophyll metabolism
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
71-hydroxychlorophyll a:ferredoxin oxidoreductase
Contains FAD and an iron-sulfur center. This enzyme, which is present in plant chloroplasts, carries out the second step in the conversion of chlorophyll b to chlorophyll a. It similarly reduces chlorophyllide a.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
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HCAR functions in Chl degradation as a component of the SGRCCE-LHCII complex during leaf senescence
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-hydroxymethyl-chlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
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ir
7-hydroxymethylchlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
71-hydroxychlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
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ir
additional information
?
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HCAR interacts with LHCII in in vivo pull-down assays, and with SGR, NYC1, NOL and RCCR in yeast two-hybrid assays
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-hydroxymethyl-chlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
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ir
7-hydroxymethylchlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
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HCAR reduces 7-hydroxymethyl chlorophyll a but not 7-hydroxymethyl pheophytin a or 7-hydroxymethyl pheophorbide a
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?
71-hydroxychlorophyll a + reduced ferredoxin + H+
chlorophyll a + oxidized ferredoxin + H2O
show the reaction diagram
Q8GS60
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ir
additional information
?
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HCAR interacts with LHCII in in vivo pull-down assays, and with SGR, NYC1, NOL and RCCR in yeast two-hybrid assays
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
reduced ferredoxin
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[4Fe-4S]-center
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Q8GS60
Arabidopsis thaliana;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
195000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
3 * 49000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 25% (w/v) polyethylene glycol 3350, 3% (v/v) ethylene glycol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex 200 gel filtration
nickel affinity column chromatography
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using Ni-NTA chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL-21 as a His-tagged fusion protein
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expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21(DE3) cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
HCAR is expressed throughout leaf development and is drastically down-regulated during dark-induced senescence
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HCAR is highly up-regulated during greening of etiolated seedlings
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the enzyme activity is induced upon senescence
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E262A
the mutant loses its catalytic activity, probably for its impaired FAD binding capacity
H417A
the mutant formes inclusion bodies during expression, and hence its activity is not measureable
Q332A
the mutant aggregates and its activity is not measureable
additional information
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the activity of HMChl reductase is partially down-regulated in mutant hmc1