The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre . Together with EC 1.12.1.2 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
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SYSTEMATIC NAME
IUBMB Comments
formate:NAD+ oxidoreductase
The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre [3]. Together with EC 1.12.1.2 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
formate dehydrogenase is a key enzyme in formaldehyde metabolism by oxidation of HCOOH to CO2, which enters the Calvin cycle to be assimilated into glucose
melting temperature, medium stability of enzyme in comparison with enzymes from Moraxella sp. C1, Pseudomonas sp. 101, Candida boidinii, and Glycine max. Irreversible inactivation according to first-order reaction kinetics
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in the chloroplasts of transgenic tobacco using a light inducible promote. Overexpression of the Arabidopsis formate dehydrogenase in chloroplasts enhances formaldehyde uptake and metabolism in transgenic tobacco leaves. Overexpression in chloroplasts fails to introduce the carbon flux of HCHO metabolism into the Calvin cycle in transgenic tobacco leaves. However, the overexpression significantly enhances the HCHO metabolism in transgenic leaves. Consequently, the productions of Asn, Gln, oxalate, and HCOOH are notably greater in transgenic leaves than in non-transformed leaves after treatment with HcHO. The increased stomatal conductance and aperture in transgenic leaves might be ascribed to the increased yield of oxalate in the guard cells with overexpressed enzyme in chloroplasts. Accordingly, the transgenic plants exhibit a stronger capacity to absorb gaseous HCHO. The higher proline content in transgenic leaves compared with non-transformed leaves under HCHO stress might be attributable to the excess formate accumulation and Gln production. Consequently, the HCHO-induced oxidative stress is reduced in transgenic leaves
production of transgenic Arabidopsis and tobacco plants that overexpress Arabidopsis formate dehydrogenase. The formate dehydrogenase specific activity in the leaf tissue of the transgenic plants increases an average of 4.5-fold for Arabidopsis and 31.5fold for tobacco
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
heating converts the native enzyme to the molten globule state and cooling results in formation of a nonnative structure with altered kinetic properties