Information on EC 1.16.9.1 - iron:rusticyanin reductase

for references in articles please use BRENDA:EC1.16.9.1
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The expected taxonomic range for this enzyme is: Acidithiobacillus ferrooxidans

EC NUMBER
COMMENTARY hide
1.16.9.1
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RECOMMENDED NAME
GeneOntology No.
iron:rusticyanin reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Fe(II) + rusticyanin = Fe(III) + reduced rusticyanin
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fe(II) oxidation
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SYSTEMATIC NAME
IUBMB Comments
Fe(II):rusticyanin oxidoreductase
Contains c-type heme. The enzyme in Acidithiobacillus ferrooxidans is a component of an electron transfer chain from Fe(II), comprising this enzyme, the copper protein rusticyanin, cytochrome c4, and cytochrome c oxidase (EC 1.9.3.1).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Fe(II) + rusticyanin
Fe(III) + reduced rusticyanin
show the reaction diagram
Fe2+ + rusticyanin
Fe3+ + reduced rusticyanin
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Fe(II) + rusticyanin
Fe(III) + reduced rusticyanin
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
rusticyanin
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49700
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calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially purified by ammonium sulfate precipitation and SP-Sephadex C-25 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene cyc2, genetic organization, quantitative real-time PCR gene expression analysis
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gene cyc2, the gene is encoded in the iron oxidation system-encoding rus operon comprising genes cyc1, cyc2, coxA, coxC and rus, quantitative real-time PCR gene expression analysis in strain CCM 4253
recombinant plasmid pTCYC2 containing cyc2 gene under the control of Ptac promoter is constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
quantitative 2D-PAGE proteomic analysis of a resting cell suspension of a sulfur-grown Acidithiobacillus ferrooxidans CCM 4253 subculture that has lost its iron-reducing activity reveals that 147 protein spots are downregulated relative to an iron-reducing resting cell suspension of the antecedent sulfur-oxidizing culture and 111 are upregulated. Gene cyc2 encoding the enzyme is strongly downregulated