Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(24R)-ergost-4-en-3-one + NADPH + H+ + O2
(22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O
2,22-dideoxyecdysone + NADPH + H+ + O2
? + NADP+ + H2O
-
-
-
-
?
campestanol + NADPH + H+ + O2
6-deoxocathasterone + NADP+ + H2O
-
catalytic efficiency for campesterol is 325 times greater than for campestanol. 17% of the activity with cholesterol
-
-
?
campesterol + NADPH + H+ + O2
(22S)-22-hydroxycampesterol + NADP+ + H2O
best substrate
-
-
?
campesterol + NADPH + H+ + O2
22alpha-hydroxycampesterol + NADP+ + H2O
-
catalytic efficiency for campesterol is 325 times greater than for campestanol. 76.9% of the activity with cholesterol
-
-
?
cholestanol + NADPH + H+ + O2
22-hydroxy-cholestanol + NADP+ + H2O
-
-
29% of the activity with cholesterol
-
?
cholesterol + NADPH + H+ + O2
22-hydroxy-cholesterol + NADP+ + H2O
-
-
-
-
?
sitosterol + NADPH + H+ + O2
22-hydroxy-sitosterol + NADP+ + H2O
-
poor substrate. 10.7% of the activity with cholesterol
-
-
?
additional information
?
-
(24R)-ergost-4-en-3-one + NADPH + H+ + O2

(22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O
17% of the activity with campesterol
-
-
?
(24R)-ergost-4-en-3-one + NADPH + H+ + O2
(22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O
7.9% of the activity with campesterol
-
-
?
additional information

?
-
-
substrates with a double bond at positions C5 and C6 are preferred substrates
-
-
?
additional information
?
-
Arabidopsis plants ectopically overexpressing DWF4 (AOD4) are generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes are characterized. The hypocotyl length of both light and dark-grown AOD4 seedlings is increased dramatically as compared to wild type. At maturity, inflorescence height increases >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced
-
-
?
additional information
?
-
DWF4 is down-regulated by jasmonate and is located downstream of COI1 in the jasmonate-signaling pathway
-
-
?
additional information
?
-
only 22alpha-hydroxylated brassinosteroids rescue the dwf4 phenotype, confirming that DWF4 acts as a 22alpha-hydroxylase
-
-
?
additional information
?
-
the 22-hydroxylation step is one of the rate-limiting steps in brassinosteroid biosynthesis
-
-
?
additional information
?
-
the regulation of DWARF4 expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in Arabidopsis
-
-
?
additional information
?
-
CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis
-
-
?
additional information
?
-
CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis
-
-
?
additional information
?
-
-
CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis
-
-
?
additional information
?
-
the enzyme catalyzes C-22 hydroxylation of (14R)-5alpha-ergostan-3-one and campestanol at a trace level
-
-
?
additional information
?
-
the enzyme catalyzes C-22 hydroxylation of (14R)-5alpha-ergostan-3-one and campestanol at a trace level
-
-
?
additional information
?
-
-
the enzyme catalyzes C-22 hydroxylation of (14R)-5alpha-ergostan-3-one and campestanol at a trace level
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ohnishi, T.; Watanabe, B.; Sakata, K.; Mizutani, M.
CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato
Biosci. Biotechnol. Biochem.
70
2071-2080
2006
Solanum lycopersicum (A0ZS62), Solanum lycopersicum (A0ZS63), Solanum lycopersicum
brenda
Asami, T.; Mizutani, M.; Fujioka, S.; Goda, H.; Min, Y.K.; Shimada, Y.; Nakano, T.; Takatsuto, S.; Matsuyama, T.; Nagata, N.; Sakata, K.; Yoshida, S.
Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta
J. Biol. Chem.
276
25687-25691
2001
Arabidopsis thaliana
brenda
Kappler, C.; Kabbouh, M.; Hetru, C.; Durst, F.; Hoffmann, J.A.
Characterization of three hydroxylases involved in the final steps of biosynthesis of the steroid hormone ecdysone in Locusta migratoria (Insecta, Orthoptera)
J. Steroid Biochem.
31
891-898
1988
Locusta migratoria
brenda
Fujita, S.; Ohnishi, T.; Watanabe, B.; Yokota, T.; Takatsuto, S.; Fujioka, S.; Yoshida, S.; Sakata, K.; Mizutani, M.
Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols
Plant J.
45
765-774
2006
Arabidopsis thaliana
brenda
Sekimata, K.; Ohnishi, T.; Mizutani, M.; Todoroki, Y.; Han, S.Y.; Uzawa, J.; Fujioka, S.; Yoneyama, K.; Takeuchi, Y.; Takatsuto, S.; Sakata, K.; Yoshida, S.; Asami, T.
Brz220 interacts with DWF4, a cytochrome P450 monooxygenase in brassinosteroid biosynthesis, and exerts biological activity
Biosci. Biotechnol. Biochem.
72
7-12
2008
Arabidopsis thaliana (O64989), Arabidopsis thaliana
brenda
Choe, S.; Dilkes, B.P.; Fujioka, S.; Takatsuto, S.; Sakurai, A.; Feldmann, K.A.
The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alpha-hydroxylation steps in brassinosteroid biosynthesis
Plant Cell
10
231-243
1998
Arabidopsis thaliana (O64989)
brenda
Choe, S.; Fujioka, S.; Noguchi, T.; Takatsuto, S.; Yoshida, S.; Feldmann, K.A.
Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Plant J.
26
573-582
2001
Arabidopsis thaliana (O64989)
brenda
Shimada, Y.; Goda, H.; Nakamura, A.; Takatsuto, S.; Fujioka, S.; Yoshida, S.
Organ-specific expression of brassinosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis
Plant Physiol.
131
287-297
2003
Arabidopsis thaliana (O64989)
brenda
Kim, H.B.; Kwon, M.; Ryu, H.; Fujioka. S.; Takatsuto, S.; Yoshida, S.; An, C.S.; Lee, I.; Hwang, I.; Choe, S.
The regulation of DWARF4 expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in Arabidopsis
Plant Physiol.
140
548-557
2006
Arabidopsis thaliana (O64989)
brenda
Ren, C.; Han, C.; Peng, W.; Huang, Y.; Peng, Z.; Xiong, X.; Zhu, Q.; Gao, B.; Xie, D.
A leaky mutation in DWARF4 reveals an antagonistic role of brassinosteroid in the inhibition of root growth by jasmonate in Arabidopsis
Plant Physiol.
151
1412-1420
2009
Arabidopsis thaliana (O64989)
brenda
Yoshimitsu, Y.; Tanaka, K.; Fukuda, W.; Asami, T.; Yoshida, S.; Hayashi, K.; Kamiya, Y.; Jikumaru, Y.; Shigeta, T.; Nakamura, Y.; Matsuo, T.; Okamoto, S.
Transcription of DWARF4 plays a crucial role in auxin-regulated root elongation in addition to brassinosteroid homeostasis in Arabidopsis thaliana
PLoS ONE
6
e23851
2011
Arabidopsis thaliana (O64989), Arabidopsis thaliana
brenda