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Information on EC 1.14.99.58 - heme oxygenase (biliverdin-IX-beta and delta-forming)

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IUBMB Comments
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, differs from EC 1.14.15.20, heme oxygenase (biliverdin-producing, ferredoxin), in that the heme substrate is rotated by approximately 110 degrees within the active site, resulting in cleavage at a different part of the ring. It forms a mixture of about 70% biliverdin-IX-delta and 30% biliverdin-IX-beta.
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This record set is specific for:
UNIPROT: O69002
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The enzyme appears in viruses and cellular organisms
Synonyms
biliverdin IXbeta/delta-selective heme oxygenase, BphO, heme-PigA heme oxygenase, heme-PigA(HO), HemO, PigA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PigA
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SYSTEMATIC NAME
IUBMB Comments
protoheme,donor:oxygen oxidoreductase (biliverdin-IX-beta and delta-forming)
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, differs from EC 1.14.15.20, heme oxygenase (biliverdin-producing, ferredoxin), in that the heme substrate is rotated by approximately 110 degrees within the active site, resulting in cleavage at a different part of the ring. It forms a mixture of about 70% biliverdin-IX-delta and 30% biliverdin-IX-beta.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
heme + 3 AH2 + 3 O2
biliverdin + Fe2+ + CO + 3 A + 3 H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
heme + 3 AH2 + 3 O2
biliverdin + Fe2+ + CO + 3 A + 3 H2O
show the reaction diagram
natural electron donor for PigA from Pseudomonas aeruginosa PAO1 is ferredoxin-NADP+-oxidoreductase
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ferredoxin-NADP+-oxidoreductase
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additional information
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
high activity of PigA within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PigA; gene pigA
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
O69002_PSEAI
198
0
21954
TrEMBL
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST-tagged PigA from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pigA, pigA genes are organized in gene clusters associated with iron utilization, expression of GST-tagged PigA in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gisk, B.; Wiethaus, J.; Aras, M.; Frankenberg-Dinkel, N.
Variable composition of heme oxygenases with different regiospecificities in Pseudomonas species
Arch. Microbiol.
194
597-606
2012
Pseudomonas aeruginosa (O69002), Pseudomonas aeruginosa (Q88P48), Pseudomonas aeruginosa (Q9HWR4), Pseudomonas aeruginosa KT 2240 (Q88P48), Pseudomonas fluorescens (Q4K657), Pseudomonas fluorescens (Q4K7S1), Pseudomonas fluorescens Pf-5 (Q4K657), Pseudomonas fluorescens Pf-5 (Q4K7S1), Pseudomonas mendocina (A4Y0Y8), Pseudomonas mendocina YMP (A4Y0Y8), Pseudomonas putida (Q88JR7), Pseudomonas putida KT 2240 (Q88JR7), Pseudomonas syringae pv. tomato (Q885D4), Pseudomonas syringae pv. tomato (Q887K9), Pseudomonas syringae pv. tomato DC3000 (Q885D4), Pseudomonas syringae pv. tomato DC3000 (Q887K9)
Manually annotated by BRENDA team