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Information on EC 1.14.99.44 - diapolycopene oxygenase and Organism(s) Staphylococcus aureus and UniProt Accession Q8NUQ6

for references in articles please use BRENDA:EC1.14.99.44
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IUBMB Comments
Little activity with neurosporene or lycopene. Involved in the biosynthesis of C30 carotenoids such as staphyloxanthin. The enzyme oxidizes each methyl group to the hydroxymethyl and then a dihydroxymethyl group, followed by the spontaneous loss of water to give an aldehyde group.
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This record set is specific for:
Staphylococcus aureus
UNIPROT: Q8NUQ6
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The taxonomic range for the selected organisms is: Staphylococcus aureus
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
crtnb, crtox, diapolycopene oxygenase, 4,4'-diapolycopene oxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4,4'-diapolycopene oxygenase
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SYSTEMATIC NAME
IUBMB Comments
4,4'-diapolycopene,AH2:oxygen oxidoreductase (4,4'-hydroxylating)
Little activity with neurosporene or lycopene. Involved in the biosynthesis of C30 carotenoids such as staphyloxanthin. The enzyme oxidizes each methyl group to the hydroxymethyl and then a dihydroxymethyl group, followed by the spontaneous loss of water to give an aldehyde group.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4,2',4'-tetradehydrolycopene + AH2 + O2
2,4,2',4'-tetradehydrolycopendial + A + H2O
show the reaction diagram
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?
4,4'-diapolycopene + AH2 + O2
4,4'-diapolycopen-4,4'-dial + A + H2O
show the reaction diagram
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?
2,4,2',4'-tetradehydrolycopene + AH2 + O2
2,4,2',4'-tetradehydrolycopene dialdehyde + 2,4,2',4'-tetradehydrolycopene monoaldehyde + A + H2O
show the reaction diagram
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?
4,4'-diapolycopene + AH2 + O2
4,4'-diapolycopene-4,4'-dial + A + H2O
show the reaction diagram
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?
4,4'-diapolycopene + AH2 + O2
4,4'-diapolycopene-4,4'-dialdehyde + A + H2O
show the reaction diagram
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?
4,4'-diaponeurosporene + AH2 + O2
4,4'-diaponeurosporen-4-al + A + H2O
show the reaction diagram
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?
4,4'-diaponeurosporene + AH2 + O2
4,4'-diaponeurosporen-4-monoaldehyde + A + H2O
show the reaction diagram
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?
additional information
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tao, L.; Schenzle, A.; Odom, J.; Cheng, Q.
Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde
Appl. Environ. Microbiol.
71
3294-3301
2005
Methylomonas sp. (Q4VKU9), Methylomonas sp. 16a (ATCC PTA-2402) (Q4VKU9), Staphylococcus aureus, Staphylococcus aureus ATCC 35556
Manually annotated by BRENDA team
Mijts, B.N.; Lee, P.C.; Schmidt-Dannert, C.
Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution
Chem. Biol.
12
453-460
2005
Staphylococcus aureus (Q8NUQ6), Staphylococcus aureus
Manually annotated by BRENDA team
Cheng, Q.
Structural diversity and functional novelty of new carotenoid biosynthesis genes
J. Ind. Microbiol. Biotechnol.
33
552-559
2006
Methylomonas sp. (Q4VKU9), Staphylococcus aureus
Manually annotated by BRENDA team