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Information on EC 1.14.99.29 - deoxyhypusine monooxygenase
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EC Tree
1.14.99.29 deoxyhypusine monooxygenaseIUBMB Comments
The enzyme catalyses the final step in the formation of the amino acid hypusine in the eukaryotic initiation factor 5A.
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Word Map
- 1.14.99.29
-
eif-5a
- spermidine
-
hypusine-containing
- gc7
- mimosine
- ciclopirox
-
nepsilon-4-amino-2-hydroxybutyllysine
- drug development
- deferiprone
-
eif5a-1
-
polyamine-derived
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4-aminobutyl
-
diiron
- n1-guanyl-1,7-diaminoheptane
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heat-repeats
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heat-like
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peroxo
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deoxyhypusine-containing
- medicine
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
deoxyhypusine hydroxylase, hdohh, deoxyhypusyl hydroxylase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
deoxyhypusine hydroxylase
deoxyhypusyl hydroxylase
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-
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DOHH
hDOHH
oxygenase, deoxyhypusine di-
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-
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additional information
deoxyhypusine hydroxylase
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-
-
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deoxyhypusine hydroxylase
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DOHH
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additional information
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DOHH belongs to a family of HEAT-like repeat proteins which comprise the Huntingtin protein, elongation factor 3, the protein phosphatase subunit 2A, and the target of rapamycin
additional information
-
DOHH belongs to a family of HEAT-like repeat proteins which comprise the Huntingtin protein, elongation factor 3, the protein phosphatase subunit 2A, and the target of rapamycin
additional information
-
the enzyme belongs to the family of 2-oxoacid- and Fe(II)-dependent dioxygenases
additional information
-
DOHH belongs to a family of HEAT-like repeat proteins which comprise the Huntingtin protein, elongation factor 3, the protein phosphatase subunit 2A, and the target of rapamycin
additional information
-
DOHH belongs to a family of HEAT-like repeat proteins which comprise the Huntingtin protein, elongation factor 3, the protein phosphatase subunit 2A, and the target of rapamycin
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[eIF5A]-deoxyhypusine + reduced acceptor + O2 = [eIF5A]-hypusine + acceptor + H2O
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[eIF5A]-deoxyhypusine + reduced acceptor + O2 = [eIF5A]-hypusine + acceptor + H2O
binuclear iron mediated reaction mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydroxylation
oxidation
redox reaction
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reduction
oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
deoxyhypusine,hydrogen-donor:oxygen oxidoreductase (2-hydroxylating)
The enzyme catalyses the final step in the formation of the amino acid hypusine in the eukaryotic initiation factor 5A.
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CAS REGISTRY NUMBER
COMMENTARY
101920-83-6
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102576-87-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of human translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of human translation initiation factor 5A + oxidized electron donor + H2O
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of yeast translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of yeast translation initiation factor 5A + oxidized electron donor + H2O
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
[eIF5A]-deoxyhypusine + NADH + H+ + O2
[eIF5A]-hypusine + NAD+ + H2O
-
-
-
-
?
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of human translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of human translation initiation factor 5A + oxidized electron donor + H2O
-
-
-
-
?
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of human translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of human translation initiation factor 5A + oxidized electron donor + H2O
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strong preference of enzyme for binding the 6-N-(4-amino-2-hydroxybutyl)-L-lysine-containing form of translation initiation factor 5A
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-
?
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of yeast translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of yeast translation initiation factor 5A + oxidized electron donor + H2O
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strong preference of enzyme for binding the 6-N-(4-amino-2-hydroxybutyl)-L-lysine-containing form of translation initiation factor 5A
-
-
?
6-N-[(R)-4-amino-2-hydroxybutyl]-L-lysine of yeast translation initiation factor 5A + electron donor + O2
6-N-(4-aminobutyl)-L-lysine of yeast translation initiation factor 5A + oxidized electron donor + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
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ir
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the DOHH reaction is irreversible
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ir
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
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activation of eIF5A
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-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist, no activity with eIF5A mutant K51R, that lacks hypusine. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival
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-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
eIF5A is the eukaryotic translation initiation factor 5A
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-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
-
the enzyme catalyzes the final step of the post-translational synthesis of hypusine, i.e. Nepsilon-(4-amino-2-hydroxybutyl)lysine, in the eukaryotic translation initiation factor 5A, eIF5A
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?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
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eIF5A is the eukaryotic translation initiation factor 5A, specificity of the interaction between eIF5A and DOHH using the isoform eIF5A-1, DOHH displays a strong preference for binding the deoxyhypusine-containing form of eIF5A, over the eIF5A precursor or the hypusine-containing eIF5A, the deoxyhypusine side chain of the substrate is primarily anchored by gamma-carboxyl groups of Glu57 and Glu208 at the DOHH active site, substrate binding modelling, overview
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-
?
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
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?
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
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-
-
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?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
N'-(4-aminobutyl)lysine + electron donor + O2
N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O
-
i.e. deoxyhypusine
i.e. hypusine
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
product analysis by GC/MS and gel filtration
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
product analysis by GC/MS and gel filtration
-
?
additional information
?
-
eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells
-
-
?
additional information
?
-
-
eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells
-
-
?
additional information
?
-
-
DOHH hydroxylates deoxyhypusine and irreversibly completes the hypusination process, the eukaryotic translation initiation factor eIF-5A is the sole known target of hypusination
-
-
?
additional information
?
-
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substrate specifc bindign of recombinant wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
DOHH hydroxylates the deoxyhypusyl-eukaryotic translation initiation factor eIF5A intermediate to hyposine-containing mature eIF5A using molecular oxygen
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-
?
additional information
?
-
DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation
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-
?
additional information
?
-
-
DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation
-
-
?
additional information
?
-
-
purified DOHH protein displays no phycocyanin lyase activity
-
-
?
additional information
?
-
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following mutants of human eIF-5A are substrates for DOHH - K47A, K47R, G49A, G52A, K55A, P74A, L91A and L101A
-
-
?
additional information
?
-
-
Lia1 contains HEAT-like repeats with a role for mediating protein-protein interactions
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
[eIF5A]-deoxyhypusine + NADH + H+ + O2
[eIF5A]-hypusine + NAD+ + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the DOHH reaction is irreversible
-
-
ir
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
activation of eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
different eIF5A isozymes exist, no activity with eIF5A mutant K51R, that lacks hypusine. The unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
-
-
-
?
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
the unique posttranslational modification occurs in only one cellular protein, the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival
-
-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2
eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
-
the enzyme catalyzes the final step of the post-translational synthesis of hypusine, i.e. Nepsilon-(4-amino-2-hydroxybutyl)lysine, in the eukaryotic translation initiation factor 5A, eIF5A
-
-
?
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
-
-
-
-
?
eukaryotic translation initiation factor eIF-5A deoxyhypusine + O2 + AH2
eukaryotic translation initiation factor eIF-5A hypusine + H2O + A
-
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
[eIF5A]-deoxyhypusine + AH2 + O2
[eIF5A]-hypusine + A + H2O
-
-
-
?
additional information
?
-
eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells
-
-
?
additional information
?
-
-
eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells
-
-
?
additional information
?
-
-
DOHH hydroxylates deoxyhypusine and irreversibly completes the hypusination process, the eukaryotic translation initiation factor eIF-5A is the sole known target of hypusination
-
-
?
additional information
?
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DOHH hydroxylates the deoxyhypusyl-eukaryotic translation initiation factor eIF5A intermediate to hyposine-containing mature eIF5A using molecular oxygen
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additional information
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DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation
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additional information
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DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation
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following mutants of human eIF-5A are substrates for DOHH - K47A, K47R, G49A, G52A, K55A, P74A, L91A and L101A
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additional information
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Lia1 contains HEAT-like repeats with a role for mediating protein-protein interactions
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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no activity with NADPH, ascorbic acid, mercaptoethylamine, mercaptoacetic acid
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Iron
additional information
Fe2+
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the enzyme contains a nonheme diiron active center that activates O2
Fe2+
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the enzyme contains a nonheme diiron active center that activates O2
Fe2+
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a non-heme iron enzyme, DOHH probably contains one binuclear, diiron, active center, iron-binding active site structure of DOHH and mode of iron binding, the strictly conserved His-Glu motifs His56-Glu57, His89-Glu90, His207-Glu208 and His240-Glu241, are involved in iron binding, overview
Fe2+
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required, essential structural role for iron binding in addition to its contribution to the catalysis of hypusine formation in the eIF-5A precursor. Lia1 is an iron metalloenzyme. Determination of metal contents in purified enzyme samples, overview. The apoenzyme has a larger hydrodynamic size than the holoenzyme
Fe2+
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the enzyme contains a nonheme diiron active center that activates O2
Fe2+
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the enzyme contains a nonheme diiron active center that activates O2
additional information
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iron cannot be replaced by Cd2+, Co2+, Cr2+, Cu2+, Mg2+, Mn2+, Ni2+, Zn2+
additional information
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Lia1 contains no cadmium, cobalt, chromium, copper, magnesium, manganese, nickel and zinc
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-(2-hydroxy-5-methylphenyl)-1,3-thiazole-4-carboxylic acid
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inhibition in vitro and in cells
2-(4-methoxyphenyl)-6-[[2-(4-methoxyphenyl)-3H-benzimidazol-5-yl]methyl]-1H-benzimidazole
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94.1% inhibition at 0.002 mM
alkyl 4-oxo-piperidine 3 carboxylates
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structurally related to dihydropyrimidines, most potent, putative DOHH inhibitors in vitro
deferoxamine
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targets the active metalloenzyme and inhibits DOHH in human vascular endothelial cells
N-phenyl-1-[1-(phenylmethyl)benzimidazol-2-yl]diazenylnaphthalen-2-amine
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87.6% inhibition at 0.002 mM
N-[4-(3,4-diethoxyphenyl)-1,2,5-oxadiazole-3-yl]-3-methylbenzamide
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92.6% inhibition at 0.002 mM
2,2'-dipyridyl
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targets the active metalloenzyme and inhibits DOHH in human vascular endothelial cells
2-(2-hydroxy-5-methylphenyl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
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inhibition in vitro and in cells
2-(2-hydroxy-5-methylphenyl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
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inhibition in vitro and in cells
ciclopirox
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targets the active metalloenzyme and inhibits DOHH in human vascular endothelial cells
Co(C2H3O2)2