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IUBMB CommentsAlso oxidizes 2,5-dihydroxypyridine, but does not act on 3-hydroxypyridine, 4-hydroxypyridine or 2,6-dihydroxypyridine.
The expected taxonomic range for this enzyme is: Brevibacillus brevis
Synonyms
2-hydroxypyridine oxygenase, more
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2-hydroxypyridine oxygenase
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2-hydroxypyridine + reduced acceptor + O2 = 2,5-dihydroxypyridine + acceptor + H2O
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2-hydroxypyridine,hydrogen-donor:oxygen oxidoreductase (5-hydroxylating)
Also oxidizes 2,5-dihydroxypyridine, but does not act on 3-hydroxypyridine, 4-hydroxypyridine or 2,6-dihydroxypyridine.
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2-hydroxypyridine + NADH + O2
2,5-dihydroxypyridine + NAD+ + H2O
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Substrates: -
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2-hydroxypyridine + NADH + O2
2,5-dihydroxypyridine + NAD+ + H2O
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NADPH
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2% activity of NADH
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FMN
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85% inhibition at 1.7 mM, 2 min preincubation
Na2As2O3
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partial inhibition
NaF
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53% inhibition at 1.7 mM, 5 min preincubation
NaN3
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53% inhibition at 1.7 mM, 5 min preincubation
NiCl2
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47% inhibition at 1.7 mM
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6.3 - 8.5
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half-maximal activity at pH 6.3 and pH 8.5
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isolated by enrichment on isonicotinic acid
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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45 mM dithiothreitol affords partial protection against denaturation
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50% glycerol affords partial protection against denaturation
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dialysis of extracts in cold, 4°C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss
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enzyme is completely blocked by anaerobic conditions
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-10°C or 0°C, overnight, complete loss of activity
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-20°C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d
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Sharma, M.L.; Kaul, S.M.; Shukla, O.P
Metabolism of 2-hydroxypyridine by Bacillus brevis (INA)
Biol. Mem.
9
43-52
1984
Brevibacillus brevis
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brenda
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