Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.14.99.1 - prostaglandin-endoperoxide synthase and Organism(s) Salvelinus fontinalis and UniProt Accession Q9PTN3

for references in articles please use BRENDA:EC1.14.99.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This enzyme acts both as a dioxygenase and as a peroxidase.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Salvelinus fontinalis
UNIPROT: Q9PTN3
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Salvelinus fontinalis
The enzyme appears in selected viruses and cellular organisms
Synonyms
cox-2, cyclooxygenase, cyclooxygenase-2, cox-1, cyclooxygenase 2, ptgs2, pghs-2, cyclooxygenase-1, prostaglandin synthetase, pghs-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
prostaglandin H synthase
-
tPGHS-1
brook trout, Salvelinus fontinalis
(PG)H synthase
-
-
-
-
cyclooxygenase
-
-
-
-
fatty acid cyclooxygenase
-
-
-
-
PG synthetase
-
-
-
-
prostaglandin endoperoxide synthetase
-
-
-
-
prostaglandin G/H synthase
-
-
-
-
prostaglandin H synthase
-
prostaglandin synthase
-
-
-
-
prostaglandin synthase-2
-
-
-
-
prostaglandin synthetase
-
-
-
-
synthase, prostaglandin
-
-
-
-
tPGHS-2
brook trout, Salvelinus fontinalis
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
reduction
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate,hydrogen-donor:oxygen oxidoreductase
This enzyme acts both as a dioxygenase and as a peroxidase.
CAS REGISTRY NUMBER
COMMENTARY hide
39391-18-9
-
9055-65-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
? + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
show the reaction diagram
-
-
-
?
cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
gamma-linolenic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoic acid + AH2 + O2
? + A + H2O
show the reaction diagram
-
-
-
?
alpha-linolenic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
show the reaction diagram
-
-
-
?
cis-5,8,11,14,17-eicosapentaenoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
cis-7,10,13,16-docosatetraenoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
cis-8,11,14-eicosatrienoic acid + reduced acceptor + O2
?
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
show the reaction diagram
-
-
-
?
arachidonate + AH2 + O2
prostaglandin H2 + A + H2O
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.082
alpha-linolenic acid
-
0.07
cis-4,7,10,13,16,19-docosahexaenoic acid
-
0.039
cis-5,8,11,14,17-eicosapentaenoic acid
-
0.013
cis-5,8,11,14-eicosatetraenoic acid
-
0.061
cis-7,10,13,16-docosatetraenoic acid
-
0.036
cis-8,11,14-eicosatrienoic acid
-
0.162
gamma-linolenic acid
-
0.0101
alpha-linolenic acid
-
0.0318
cis-4,7,10,13,16,19-docosahexaenoic acid
-
0.0153
cis-5,8,11,14,17-eicosapentaenoic acid
-
0.0024
cis-5,8,11,14-eicosatetraenoic acid
-
0.0357
cis-7,10,13,16-docosatetraenoic acid
-
0.0059
cis-8,11,14-eicosatrienoic acid
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2300
cyclooxygenase activity for partially purified prostaglandin H synthase 1 of Salvelinus fontinalis, tPGHS-1
15000
cyclooxygenase activity for partially purified prostaglandin H synthase 2 of Salvelinus fontinalis, tPGHS-2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
cyclooxygenase activity assay
8
lipoxygenase activity assay
7.2
cyclooxygenase activity assay
8
lipoxygenase activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
room temperature, 22-23°C, lipoxygenase activity assay
25
cyclooxygenase activity assay using a standard electrode
30
cyclooxygenase activity assay using a high sensitivity electrode
23
room temperature, 22-23°C, lipoxygenase activity assay
25
cyclooxygenase activity assay using a standard electrode
30
cyclooxygenase activity assay using a high sensitivity electrode
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PGHS-1
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
distribution in different organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q9PTN3_SALFO
598
0
68676
TrEMBL
Secretory Pathway (Reliability: 3)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column
recombinant protein expressed in Sf9 cells, protein is purified from the membrane fraction using a High Q ion exchange column
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Spodoptera frugiperda Sf-9 cells
into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells
expression in Spodoptera frugiperda Sf-9 cells
into a pVL1392 vector for transformation of Escherichia coli DH5alpha competent cells
isozymes 1 and 2
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roberts, S.B.; Langenau, D.M.; Goetz, F.W.
Cloning and characterization of prostaglandin endoperoxide synthase-1 and -2 from the brook trout ovary
Mol. Cell. Endocrinol.
160
89-97
2000
Salvelinus fontinalis
Manually annotated by BRENDA team
Liu, W.; Cao, D.; Oh, S.F.; Serhan, C.N.; Kulmacz, R.J.
Divergent cyclooxygenase responses to fatty acid structure and peroxide level in fish and mammalian prostaglandin H synthases
FASEB J.
20
1097-1108
2006
Ovis aries, Homo sapiens (P23219), Homo sapiens (P35354), Salvelinus fontinalis (Q9PTN3), Salvelinus fontinalis (Q9PW89), Salvelinus fontinalis
Manually annotated by BRENDA team