Information on EC 1.14.21.4 - salutaridine synthase

for references in articles please use BRENDA:EC1.14.21.4
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The expected taxonomic range for this enzyme is: Papaver somniferum

EC NUMBER
COMMENTARY hide
1.14.21.4
transferred to EC 1.14.19.67
RECOMMENDED NAME
GeneOntology No.
salutaridine synthase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoquinoline alkaloid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-reticuline + NADPH + H+ + O2
salutaridine + NADP+ + 2 H2O
show the reaction diagram
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?
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
salutaridine synthase (PsSAS), salutaridine reductase (PsSAR) and salutaridinol acetyltransferase (PsSAT) are functionally coexpressed in Saccharomyces cerevisiae and optimization of the pH conditions allowed for productive spontaneous rearrangement of salutaridinol-7-O-acetate and synthesis of thebaine from (R)-reticuline. A 7-gene pathway for the production of codeine and morphine from (R)-reticuline is reconstituted. Yeast cell feeding assays using (R)-reticuline, salutaridine or codeine as substrates show that all enzymes are functionally coexpressed in yeast and that activity of salutaridine reductase and codeine-O-demethylase likely limit flux to morphine synthesis. The results of this study describe a significant advance for the synthesis of morphinans in Saccharomyces cerevisiae and pave the way for their complete synthesis in recombinant microbes