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Information on EC 1.14.20.6 - flavonol synthase and Organism(s) Citrus unshiu and UniProt Accession Q9ZWQ9

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IUBMB Comments
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively . Requires Fe2+.
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This record set is specific for:
Citrus unshiu
UNIPROT: Q9ZWQ9
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Word Map
The taxonomic range for the selected organisms is: Citrus unshiu
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
flavonol synthase, sbfls, mcfls, bnfls, ppfls, vcfls, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
146359-76-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dihydrokaempferol + 2-oxoglutarate + O2
kaempferol + succinate + CO2 + H2O
show the reaction diagram
dihydroquercetin + 2-oxoglutarate + O2
quercetin + succinate + CO2 + H2O
show the reaction diagram
(+)-trans-dihydrokaempferol + 2-oxoglutarate + O2
kaempferol + succinate + CO2 + H2O
show the reaction diagram
-
-
-
-
?
(2R)-naringenin + 2-oxoglutarate + O2
(-)-trans-dihydrokaempferol + succinate + CO2 + H2O
show the reaction diagram
-
-
-
-
?
(2S)-naringenin + 2-oxoglutarate + O2
kaempferol + succinate + CO2 + H2O
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
required, Km-value: 0.011 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
2-oxoglutarate
-
0.045
dihydrokaempferol
-
0.272
dihydroquercetin
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
reaction with dihydroquercetin
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
reaction with dihydroquercetin
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Satsuma orange
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the level of the CitFLS transcript is high at the early develpmental stage and low at the mature stage in the juice sacs/segment epidermis (edible part)
Manually annotated by BRENDA team
-
the level of the CitFLS transcript increases in the peel during fruit maturation
Manually annotated by BRENDA team
-
the level of the CitFLS transcript is higher in the young leaves than in the old leaves
Manually annotated by BRENDA team
-
the level of the CitFLS transcript increases in the peel during fruit maturation
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FLS_CITUN
335
0
37899
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37899
x * 37899, MALDI-TOF mass spectrometry
38000
x * 38000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G261A
mutant enzyme with 10% of wild-type activity
G261P
no immunoreactive FLS polypeptide detected
G68A
mutant enzyme with 6% of wild-type activity
G68P
no immunoreactive FLS polypeptide detected
P207G
no effect on activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wellmann, F.; Lukacin, R.; Moriguchi, T.; Britsch, L.; Schiltz, E.; Matern, U.
Functional expression and mutational analysis of flavonol synthase from Citrus unshiu
Eur. J. Biochem.
269
4134-4142
2002
Citrus unshiu (Q9ZWQ9), Citrus unshiu
Manually annotated by BRENDA team
Moriguchi, T.; Kita, M.; Ogawa, K.; Tomono, Y.; Endo, T.; Omura, M.
Flavonol synthase gene expression during citrus fruit development
Physiol. Plant.
114
251-258
2002
Citrus unshiu, Citrus unshiu Marc.
Manually annotated by BRENDA team
Lukacin, R.; Wellmann, F.; Britsch, L.; Martens, S.; Matern, U.
Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase
Phytochemistry
62
287-292
2003
Citrus unshiu
Manually annotated by BRENDA team