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Information on EC 1.14.19.76 - flavone synthase II and Organism(s) Glycine max and UniProt Accession E9KBR8

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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein found in plants. The rice enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates. cf. EC 1.14.20.5, flavone synthase I.
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This record set is specific for:
Glycine max
UNIPROT: E9KBR8
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Word Map
The taxonomic range for the selected organisms is: Glycine max
The enzyme appears in selected viruses and cellular organisms
Synonyms
flavone synthase ii, fnsii, fns ii, cyp93g1, dvfns, cyp93b2, osfnsii, sbfnsii, afns2, tfns5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP93B16
D3U591
-
FNS II
D3U591
-
CYP93B16
-
-
-
-
CYP93G1
-
-
-
-
FNS II
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
flavanone,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (flavone-forming)
A cytochrome P-450 (heme-thiolate) protein found in plants. The rice enzyme channels flavanones to the biosynthesis of tricin O-linked conjugates. cf. EC 1.14.20.5, flavone synthase I.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
naringenin + [reduced NADPH-hemoprotein reductase] + O2
apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
luteolin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
D3U591
-
-
-
?
liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
7,4'-dihydroxyflavone + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
D3U591
-
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
apigenin + [oxidized NADPH-hemoprotein reductase] + 2 H2O
show the reaction diagram
D3U591
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
D3U591
heme-thiolate protein
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0018
eriodictyol
D3U591
pH 7.9, 25°C
0.0042
liquiritigenin
D3U591
pH 7.9, 25°C
0.0025
naringenin
D3U591
pH 7.9, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.9
D3U591
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
D3U591
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
D3U591
bound to
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
D3U591
phylogenetic analyses of the subfamily of plant CYP93B enzymes indicate the evolution of a gene encoding a flavone synthase which originally catalyzes the direct conversion of flavanones into flavones, via early gene duplication into a less efficient enzyme with an altered catalytic mechanism. Ultimately, this allows the evolution of the legume-specific isoflavonoid synthase activity
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C9B16_SOYBN
527
1
59580
Swiss-Prot
Secretory Pathway (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
D3U591
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induced by coronalon and by 12-oxo-phytodienoic acid. Jasmonic acid at a concentration of 0.1 mM is a comparatively weak inductor
D3U591
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fliegmann, J.; Furtwaengler, K.; Malterer, G.; Cantarello, C.; Schueler, G.; Ebel, J.; Mithoefer, A.
Flavone synthase II (CYP93B16) from soybean (Glycine max L.)
Phytochemistry
71
508-514
2010
Glycine max (D3U591), Glycine max
Manually annotated by BRENDA team